昆虫学报 ›› 2017, Vol. 60 ›› Issue (1): 1-8.doi: 10.16380/j.kcxb.2017.01.001

• 研究论文 •    下一篇

西花蓟马取食对菜豆不同部位叶片防御基因表达的影响

张羽宇, 郅军锐*, 刘勇, 叶茂   

  1. (贵州大学昆虫研究所, 贵州省山地农业病虫害重点实验室, 贵阳 550025)
  • 出版日期:2017-01-20 发布日期:2017-01-20

Frankliniella occidentalis (Thysanoptera: Thripidae) infestation on the expression of defensive genes in leaves at different parts of kidney bean plant

ZHANG Yu-Yu, ZHI Jun-Rui*, LIU Yong, YE Mao   

  1. (Guizhou Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China)
  • Online:2017-01-20 Published:2017-01-20

摘要: 【目的】本研究旨在探究锉吸式口器害虫西花蓟马Frankliniella occidentalis取食诱导的菜豆Phaseolus vulgaris木植株系统防御反应的分子机制。【方法】利用荧光定量PCR技术,检测西花蓟马分别在稳定取食菜豆植株2 h后的24, 48, 72和96 h菜豆植株不同部位(上部、中部和下部)叶片中茉莉酸信号转导途径和水杨酸信号转导途径防御相关基因脂氧合酶基因(LOX)、苯丙氨酸解氨酶基因(PAL)和β-1,3-葡聚糖酶基因(PR-2)相对表达量的变化。【结果】西花蓟马取食菜豆植株后,受害叶片及上部和下部健康叶片中LOXPALPR-2均被显著诱导表达,均以受害叶片防御酶基因表达量的变化较大,并且表达量在相同叶片不同时间下的变化不同。中部受害叶片中LOX的相对表达量均显著高于未接虫的对照植株,并随西花蓟马为害时间的延长逐渐升高,在96 h时为对照的209.54倍;随着时间的延长LOX表达量在上部和下部健康叶片呈现升高-降低-升高的趋势。PAL的表达量在西花蓟马为害植株的中部受害叶片和上部健康叶片均于48 h时出现最大值,分别为对照的52.70倍和41.20倍;但在下部健康叶片于72 h时达到最大值,为对照的47.06倍。PR-2表达量在受害株的上部健康叶片于48 h时出现最大值,在中部受害叶片和下部健康叶片均于96 h时达到最大值,但在24 h时在下部健康叶片中受到抑制。【结论】西花蓟马取食能显著诱导菜豆植株茉莉酸和水杨酸信号转导途径相关防御酶基因LOX, PALPR-2的表达,并在菜豆植株不同部位叶片产生系统抗性。

关键词: 西花蓟马, 系统防御, 茉莉酸途径, 水杨酸途径, 防御基因

Abstract: 【Aim】 This study aims to investigate the molecular mechanisms of systemic defense of kidney bean (Phaseolus vulgaris) plants fed by the western flower thrips, Frankliniella occidentalis, which is a kind of insect with rasping-sucking mouthparts. 【Methods】 Real-time quantitative PCR was used to detect the expression levels of lipoxygenase gene (LOX), phenylalanine ammonialyase gene (PAL) and β-1, 3-glucanase gene (PR-2), which are involved in jasmonic acid and salicylic acid signal transduction pathways, in leaves at different parts (upper, middle and lower parts) of kidney bean plant at different time (24, 48, 72 and 96 h) after feeding steadily by western flower thrips for 2 h. 【Results】 The expression levels of LOXPAL and PR-2 in the damaged middle leaves, the healthy upper and lower leaves of kidney bean plant were all significantly induced by western flower thrips, but changed more rapidly in the damaged middle leaves. The expression modes of genes changed with time. The relative expression level of LOX in the damaged middle leaves was significantly higher than that of the control (non-inoculated leaves), and increased with the time extension, which was 209.54-fold as high as that of the control at 96 h. The expression level of LOX increased first and then decreased and finally rose again in the healthy upper and lower leaves. The expression level of PAL reached the maximum in both damaged middle leaves and healthy upper leaves at 48 h after infestation of western flower thrips, which were 52.70- and 41.20-fold as high as that of the control, respectively. However, its expression level reached the peak at 72 h in the healthy lower leaves, which was 47.06-fold as high as that of the control. The expression level of PR-2 reached the maximum at 48 h after infestation of western flower thrips in the healthy upper leaves, at 96 h in both damaged middle leaves and healthy lower leaves.  PR-2 was barely expressed in the healthy lower leaves at 24 h after infestation of western flower thrips. 【Conclusion】 Western flower thrip infestation could induce the expression of defensive genes LOX PAL and PR-2 involved in jasmonic acid and salicylic acid signal transduction pathways and result in systemic resistance in leaves at different parts of kidney bean.

Key words: Frankliniella occidentalis, systemic defense, jasmonic acid pathway, salicylic acid pathway, defense genes