Characterization of three new members of the<em> apidermin</em> (<em>apd</em>) gene family from honeybees and sequence analysis of the insect APD family
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2012, Vol. 55 Issue (1): 12-23    DOI:
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Characterization of three new members of the apidermin (apd) gene family from honeybees and sequence analysis of the insect APD family
 SUN  Liang-Xian, HUANG  Zhou-Ying, ZHENG  Hua-Jun, GE  Qing-Xiu, GONG  Li-Ping, CHEN  Huai-Yu
The Higher Educational Key Laboratory for Molecular Biology and Pharmacology of Fujian Province, Quanzhou Normal University, Quanzhou, Fujian 362000, China
 Download: PDF (20537 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract The apidermin (APD) protein family is a novel structural cuticular protein family of insects. To gain a better understanding of this protein family, by using bioinformatics and RT-PCR amplification, we identified three novel apd genes (apd-1-like, apd-3-like from Apis mellifera ligustica, and apd-2 from Apis cerana cerana) and investigated their structural features, and then we revealed the characteristic motifs of the APD family. The results showed that a cluster of six apd genes were tandemly arranged on chromosome 4 of Apis mellifera. These apd genes were differentially expressed in drone head of A. m. ligustica, and their expression pattern was consistent with that of the cis-acting elements in their promoter sequences. The genomic DNA of apd-2 from A .c. cerana and apd-1-like from A. m. ligustica contain three exons and two introns, while that of apd-3-like from A. m. ligustica contain four exons and three introns. As the deduced proteins of the apd genes were analyzed, it was found that the presently available 10 APDs possess similar N-terminal signal peptide sequences. The mature APD proteins ranged in size from 6.0 to 37.0 kD, and their pI ranged from 6.2 to 10.8. Intriguingly, five small APD proteins, including APD-2 from A. cerana, and APD 1-3 and APD-like from A. mellifera, were rich in hydrophobic amino acid residues (52%-67%)  with Ala being the most abundant (25%-34%). However, the other five larger APDs, including APD 1-3 from Nasonia vitripennis, and APD-1-like and APD-3-like from A. mellifera, were Gly-rich (21%-30%) proteins with hydrophobic amino acid residues constituting 35%-41% of their amino acid sequences. Multiple alignment and phylogenetic analysis revealed that the APD protein family could be classified into two subfamilies. Subfamily Ⅰ, which contains four low complexity sequences (APD 1-3 from A. mellifera and APD-2 from A. cerana), was characterized by a 33-aa N-terminal conserved motif. The other six larger APD proteins were classified as subfamilyⅡ, which was featured by a 50-aa N-terminal motif and a 16-aa C-terminal motif. The diagnostic features of APD protein family described here will be very helpful for identifying novel apd genes from arthropods.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
SUN Liang-Xian
HUANG Zhou-Ying
ZHENG Hua-Jun
GE Qing-Xiu
GONG Li-Ping
CHEN Huai-Yu
Key wordsApis mellifera   Apis cerana   cuticular protein   apidermin gene   APD family   sequence analysis   bioinformatical analysis     
Received: 2011-09-13;
Corresponding Authors: SUN Liang-Xian   
 E-mail: lxsunbee@163.com
Cite this article:   
SUN Liang-Xian,HUANG Zhou-Ying,ZHENG Hua-Jun et al. Characterization of three new members of the apidermin (apd) gene family from honeybees and sequence analysis of the insect APD family[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(1): 12-23.
URL:  
http://www.insect.org.cn/EN/      or     http://www.insect.org.cn/EN/Y2012/V55/I1/12
 
No references of article
[1] LIU Ya-Ting, XIE Wen, ZHANG You-Jun. Research progress in sex determination in insects[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(4): 437-444.
[2] TAO Yun-Li, GUO Ya-Nan, WANG Jing, LI Li-Li, YU Yi, CHU Dong. Detection and identification of  Wolbachia in Bradysia odoriphaga  (Diptera: Sciaridae) populations from Shandong Province, China[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(4): 454-459.
[3] YUAN Xiang-Qun, GAO Ke, YUAN Feng, ZHANG Ya-Lin. Phylogenetic relationship of hesperiine tribes (Lepidoptera, Hesperiidae) based on Cytb and  ND1 gene sequences[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(4): 400-407.
[4] LIU Pei-Wen, LI Xiao-Cong,GU Jin-Bao, LIU Yan, CHEN Xiao-Guang. Molecular cloning, characterization and expression analysis of sex determiantion gene  doublesex  from  Anonpheles gambiae  (Diptera: Culicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(2): 122-131.
[5] LIANG Xin, CHEN Bin, QIAO Liang. Research progress in insect cuticular protein genes[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(9): 1084-1093.
[6] ZHONG Bao-Zhu, Lü Chao-Jun, QIAN Jun, QIN Wei-Quan, GOU Zhi-Hui.
Oviposition deterrence and ovicidal activity of Mikania micrantha extracts on Tirathaba rufivena (Lepidoptera: Pyralidae)
[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(9): 1112-1116.
[7] WU Xiao-Bo, WANG Zi-Long, LI Shu-Yun, GAN Hai-Yan, LIU Hao, YAN Wei-YU, ZENG Zhi-Jiang. Transcriptomic analysis of Apis cerana cerana (Hymenoptera: Apidae) queens and drones newly emerged and sexually matured[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(8): 905-913.
[8] FU Ming-Yue, CHEN Peng, DAI Fang-Yin, LU Cheng, TONG Xiao-Ling. Advances in the genes associated with the development of ventral limbs in insects[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(8): 962-968.
[9] . High-throughput transcriptome sequencing technology and its applications in Lepidoptera[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(8): 991-1000.
[10] SUN Meng, LU Ming-Xing, TANG Xiao-Tian, DU Yu-Zhou. Molecular cloning and expression profiling of HSC70 gene in Sesamia inferens (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(7): 787-797.
[11] DI Ning, ZHANG Xian-Zhong, ZHANG Kai, ZHANG Shi-Ze, LIU Tong-Xian. Influences of indole-3-acetic acid (IAA) spraying on Solanum lycopersicum plants and the infesting Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae) whiteflies[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(7): 824-830.
[12] TANG Yao, QIAO Liang, ZHANG Yu-Juan, CHE Yan-Fei, HONG Rui, CHEN Bin. Identification and bioinformatics analysis of genes of the CYP6Y subfamily in Anopheles sinensis (Diptera: Culicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(6): 663-672.
[13] CAI Yu-Yin, WU Qiang, LIU Gui-Qing, Lü Zhi-Chuang, LI Jian-Wei, ZHANG Gui-Fen, WAN Fang-Hao. Cloning and developmental expression analysis of an apoptosis gene hid in the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(6): 673-680.
[14] . Molecular cloning and analysis of aspartic protease (AP) gene in Ty3/gypsy retrotransposon in different geographical populations of Chilo suppressalis (Lepidoptera: Pyralidae) in China[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(5): 530-537.
[15] XU Wen, LUO Lin-Lin, WU Hua-Jun, WEI Chun-Mei, LIN Tong. Cloning and expression profiling of a cuticular protein gene in Monochamus alternatus (Coleoptera: Cerambycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(5): 515-521.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech