Species identification of Noctuidae moths (Insecta: Lepidoptera) from Baihuashan, Beijing, China with DNA barcoding
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ACTA ENTOMOLOGICA SINICA  2012, Vol. 55 Issue (9): 1082-1092    DOI:
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Species identification of Noctuidae moths (Insecta: Lepidoptera) from Baihuashan, Beijing, China with DNA barcoding
YANG Cong-Hui, HAN Hui-Lin, CHI Mei-Yan, JIN Qian, WU Chun-Sheng, ZHU Chao-Dong, ZHANG Ai-Bing
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Abstract To explore the feasibility of DNA barcoding in the identification of Noctuidae moths, the COI genes of 75 samples belonging to 43 species from Noctuidae in Baihuashan, Beijing, were amplified using universal barcoding primers. The intraspecies and interspecies genetic distances were calculated using the Kimura-2-parameter model, the phylogenetic trees were reconstructed by neighbor-joining (NJ) and maximum parsimony (MP) method, and the identification of MOTU was performed using sequence divergence threshold technique. The results showed that all of Noctuidae species were successfully distinguished by the phylogenetic tree. The average intraspecific genetic distance (0.02%) was much less than the average interspecific genetic distance (11.28%). Seventy-five Noctuidae samples were divided into 42 MOTUs based on 1% sequence divergence threshold and the accuracy rate was 95%. Most of MOTUs (41/42) were classified into their corresponding morphospecies with only one exception (MOTU04). The study indicates that the species from Noctuidae family can be well distinguished with the commonly used COI barcodes, which are potentially well utilized in moth species identification.
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YANG Cong-Hui
HAN Hui-Lin
CHI Mei-Yan
JIN Qian
WU Chun-Sheng
ZHU Chao-Dong
ZHANG Ai-Bing
Key wordsLepidoptera   noctuid   molecular taxonomy   DNA barcoding   phylogenetic tree   genetic distance   molecular defined operational taxonomic unit (MOTU)     
Received: 2012-07-25;
Corresponding Authors: ZHANG Ai-Bing   
 E-mail: zhangab2008@mail.cnu.edu.cn
About author: onionyc@126.com
Cite this article:   
YANG Cong-Hui,HAN Hui-Lin,CHI Mei-Yan et al. Species identification of Noctuidae moths (Insecta: Lepidoptera) from Baihuashan, Beijing, China with DNA barcoding[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(9): 1082-1092.
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http://www.insect.org.cn/EN/      or     http://www.insect.org.cn/EN/Y2012/V55/I9/1082
 
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