Abstract 【Aim】 To screen cold-tolerant and phylloxera (Daktulosphaira vitifoliae Fitch)-resistant grape rootstocks for grapevine industry. 【Methods】 Laboratory bioassays were conducted on 45 strains (series A) of hybrid combination Vitis amurensis Rupr. Zuoshan No.1×SO4 and 27 strains (series B) of hybrid combination V. amurensis Rupr. Zuoshan No.1×101-1 to evaluate their resistance classes to phylloxera. A system of differential thermal analysis (DTA) was used for low temperature exotherms (LTE) analysis of roots of these strains， and the temperature-injury (LT-I) regression functions were established to evaluate the cold hardiness of roots. 【Results】 Phylloxera produced less eggs on these hybrid strains than on the susceptible strain Kyoho， and 18 strains from series A and 11 strains from series B with no tuberosities were classified with the resistance class 0， and nine varieties from series A and four varieties from series B with less than 10% tuberosities were classified with the resistance class 1. Twenty-seven strains from series A and three strains from series B, whose root， phloem and xylem subordination values were all lower than those of Beta, were selected for phylloxera resistance comparison. 【Conclusion】 In this study 15 strains from series A and 2 strains from series B with high phylloxera resistance and cold hardiness were selected， and the resistance class of A14， A16， A18， A22， A23， A28， A34， A35， A38， A44， A50， B24 and B26 was 0， and that of A11， A15， A17 and A27 was 1.