Pupal morphogenesis of <em>Lucilia cuprina</em> (Diptera: Calliphoridae) under natural conditions and its significance in forensic medicine
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (2): 222-228    DOI: doi:10.16380/j.kcxb.2017.02.012
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Pupal morphogenesis of Lucilia cuprina (Diptera: Calliphoridae) under natural conditions and its significance in forensic medicine
WANG He1, WANG Hui2, WANG Wei-Jie1, LI Jia3, ZHAO Wen-Ai1, QI Li-Li1,*
(1. Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, China; 2. First Hospital of Hebei Medical University,Shijiazhuang 050000, China; 3. Department of Teaching Affairs, Clinical College of Hebei Medical University, Shijiazhuang 050000, China)
 Download: PDF (2561 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to examine the changing trend of puparium color and the pupal morphogenesis of Lucilia cuprina so as to provide relatively systematic pupal developmental data of this insect for the estimation of the postmortem interval (PMI). 【Methods】 Ten pupae were sampled at 12 h intervals from prepupa to emergence under natural conditions. The sampled pupae were fixed and observed, and the corresponding color indexes of puparium were collected and analyzed by digital image processing system. Then the pupae were dissected and their puparia were removed, and the external morphological changes of the pupae were observed and photographed under stereomicroscope. The morphological characteristics of pupae were described. 【Results】 The color of L. cuprina puparium gradually deepened with the developmental time, and obvious changes were observed at the beginning and final time of the pupal period. Based on the analyzed RGB indexes, a standard color plate of puparium was made. The pupal morphogenesis can be divided into eight stages, including cryptocephalic stage, phanerocephalic stage, brown thorax setae stage, tanned thorax setae stage, tanned abdomen setae stage, part pigmented eye stage, entire pigmented eye stage and preadult stage. 【Conclusion】 At the crime scene, the standard color plate of puparium can be used to preliminarily estimate the pupal stage through visual comparison, and based on the chronometrical morphology of L.cuprina pupae, the postmortem interval can be deduced.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
Key wordsLucilia cuprina   forensic entomology   necrophagous insect   postmortem interval   pupa   morphogenesis   standard color plate     
Cite this article:   
WANG He, Wang-Hui, Wang-Wei-Jie et al. Pupal morphogenesis of Lucilia cuprina (Diptera: Calliphoridae) under natural conditions and its significance in forensic medicine[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 222-228.
http://www.insect.org.cn/EN/doi:10.16380/j.kcxb.2017.02.012      or     http://www.insect.org.cn/EN/Y2017/V60/I2/222
No references of article
[1] JIANG Lu, HUA Bao-Zhen. Morphology of the immature stages of Panorpa macrostyla  Hua (Mecoptera: Panorpidae) with notes on its biology[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 1004-1012.
[2] YU Qin, WANG Fang, ZHANG Run-Xiang, GUO Gui-Ming, FAN Ren-Jun, HAO Chi. Effects of pupal weight on the fecundity and longevity of adults and the larval development of the next generation in Grapholitha molesta (Lepidoptera: Tortricidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 985-990.
[3] LI Pei-Guang, QIU Shi-Qi, YE Bao-Hua, WANG Ning-Xin, HUANG Da-Wei. Influence of removing Wolbachia on the reproductive fitness and adult longevity of Nasonia vitripennis (Hymenoptera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(9): 966-972.
[4] CAO Jing , OUYANG Fang, GE Feng, YANG Zhong-Xia, XIAO Tie-Guang. Hardiness characteristics of the overwintering generation of Helicoverpa armigera (Lepidoptera: Noctuidae) feeding on Bt cotton and corn[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(1): 45-52.
[5] ZHU Cheng-Jie, HE Zhang, CHEN Wei, ZHANG Shi-Lin, HU Hao-Yuan. Comparison of life table parameters of the laboratory population of Spalangia endius (Hymenoptera: Pteromalidae) on frozenkilled and fresh housefly pupae[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(10): 1219-1226.
[6] QIAN Cen, FANG Qi, WANG Lei, YE Gong-Yin. Functional studies of pacifastin proteinase inhibitor genes, nvpp-1 and nvpp-2, in Nasonia vitripennis (Hymenoptera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(8): 841-853.
[7] XIA Shi-Yang, MENG Ling, LI Bao-Ping. Effects of female body size on oviposition strategy in the gregarious parasitoid Pteromalus puparum (Hymenopera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(9): 1069-1074.
[8] WANG Huan, LI Kai, FANG Qi, YE Gong-Yin. Prokaryotic expression and thermo-protective function of heat shock proteins from Pteromalus puparum (Hymenoptera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(8): 903-910.
[9] CHEN Wen-Ting, LIU Hai-Li, YAO Li-Gong, YE Zhu-Liang, GUO Yue-Wei. Assay and comparison of chemical constituents of crude methanol extracts from different developmental stages of darkling beetle Zophpbas morio (Coleoptera: Tenebrionidae)[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(3): 357-360.
[10] HU Li-Yu, MA Ling, HU Li-Jun, JIANG Yuan, WANG Hui. Comparison of composition and content of fatty acids extracted from pupae of Dendrolimus superans (Lepidoptera: Lasiocampidae) with combinations of different extraction solvents and methods[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(1): 116-121.
[11] ZHANG Qian-Qian, WANG Fei, FANG Qi, YE Gong-Yin. In vitro cellular responses of Pieris rapae (Lepidoptera: Pieridae) hemocytes and the effects of Pteromalus puparum venom[J]. ACTA ENTOMOLOGICA SINICA, 2011, 54(11): 1264-1273.
[12] TAN Xian-Sheng, WANG Zhi-Ming, LI Lan-Zhi, YUAN Zhe-Ming. Estimating pupal developmental duration of Helicoverpa armigera (Lepidoptera: Noctuidae) with support vector regression[J]. ACTA ENTOMOLOGICA SINICA, 2011, 54(1): 83-88.
[13] ZHU Jia Ying, YE Gong Yin, FANG Qi, LI Yan Min, HU Cui. Transcriptional changes of cytoskeleton related proteins of Pieris rapae (Lepidoptera: Pieridae) after parasitization by the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2010, 53(8): 831-840.
[14] Lü Zhou, LI Xue-Bo, MO Yao-Nan. Research advances in the effects of drugs and poisons on the growth and development of necrophagous flies[J]. ACTA ENTOMOLOGICA SINICA, 2010, 53(4): 464-469.
[15] GAO Xiao-Yu, HE Y Q, ZHAO Y, ZENG F, YANG L L, XIAO C. Behavioural responses of Nassophasis sp. (Coleoptera: Dryophthoridae) adults to different odor sources[J]. ACTA ENTOMOLOGICA SINICA, 2009, 52(7): 814-819.
Supported by:Beijing Magtech