Changes in the resistance to nucleopolyhedrovirus and the expression levels of the related antimicrobial peptide and heat shock protein genes in <em>Bombyx mori</em> exposed to short-time heat shock
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (5): 505-512    DOI: 10.16380/j.kcxb.2017.05.002
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Changes in the resistance to nucleopolyhedrovirus and the expression levels of the related antimicrobial peptide and heat shock protein genes in Bombyx mori exposed to short-time heat shock
TANG Fen-Fen1, 2, ZHENG Ya-Qiang1, BAI Xing-Rong2,*, CHEN Bin1,*
(1. State Key Laboratory for Conservation and Utilization of BioResources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan 661101, China)
 Download: PDF (2718 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to investigate the effect of short-time heat shock on the resistance of the silkworm, Bombyx mori to nucleopolyhedrovirus, so as to provide references for further study on the antiviral immune mechanism of the silkworm in response to heat shock. 【Methods】 The 5th instar larvae of B. mori were exposed to 42℃ for 15 min, and then fed with B. mori nucleopolyhedrovirus (BmNPV) (5.4×106 PIBs/individual). All the tested larvae were reared as usual. The transcription levels of seven major antimicrobial genes (BmcecD, Bmmor, Bmglve2, BmdefeB, Bmatta1, Bmenbo2 and Bmlebo3) and six heat shock protein genes (Bmhsp90, Bmhsp70, Bmhsp40, Bmhsp19.9, Bmhsp21.4 and Bmhsp25.4) in the midgut of the 5th instar larvae after heat-shock treatment were analyzed by quantitative real-time PCR. The amino acid sequences of all BmHSPs were also phylogenetically analyzed using MEGA 5.0. 【Results】 Short-time heat shock significantly increased the survival rate of the BmNPV-infected 5th instar larvae of B. mori, as compared with the nonheat shock group. Quantitative real-time PCR results showed that the expression levels of Bmglve2 and Bmhsp25.4 were significantly up-regulated in the midgut of the BmNPV-infected 5th instar larvae of B. mori exposed to heat shock in comparison with the non-heat shock groups. The phylogenetic analysis showed that BmHSP25.4 was special in evolution, and individually classified into the same cluster with large HSPs (BmHSP90, BmHSP70 and BmHSP40). 【Conclusion】 Short-time heat shock before BmNPV feeding can enhance the resistance of B. mori to BmNPV. Bmhsp25.4 and Bmglve2 may relatively function in the immunity induction of B. mori against BmNPV through heat shock response.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
TANG Fen-Fen
ZHENG Ya-Qiang
BAI Xing-Rong
CHEN Bin
Key words Bombyx mori   heat shock   BmNPV   antimicrobial peptide   heat shock protein   mRNA expression level     
Cite this article:   
TANG Fen-Fen,ZHENG Ya-Qiang,BAI Xing-Rong et al. Changes in the resistance to nucleopolyhedrovirus and the expression levels of the related antimicrobial peptide and heat shock protein genes in Bombyx mori exposed to short-time heat shock[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 505-512.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.05.002      or     http://www.insect.org.cn/EN/Y2017/V60/I5/505
 
No references of article
[1] CUI Le, CHENG Rui, JIANG Nan. Lemoniidae are not distributed in China: the moth identified as Lemonia taraxaci (Lepidoptera: Leomoniidae) in China is actually a bombycid species (Lepidoptera: Bombycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 730-.
[2] YANG Ji-Gui, PENG Li-Na, LIU Tai-Xing, WU Yun-Fei, DU Guo-Yu, ZHANG Qian, WANG Wei, PAN Min-Hui. Cloning and expression analysis of the Sox family gene BmDichaete in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 513-522.
[3] WEI Chang-Ping, JIANG Jian, LI Xiang-Rui, ZHANG Yun-Hui, ZHANG Fang-Mei, CHENG Deng-Fa, GUO Wen-Chao, LIU Huai. Cloning and sequence analysis of heat shock protein gene Ld-HSP60 in Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) and its expression under temperature stress[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 523-532.
[4] BAO Xi-Yan, Chen-Peng, LIU Tai-Xing, Wang-La, Liu Wen-Bo, Pan Min-Hui, Lu-Cheng. Advances in apoptosis-related genes in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 487-498.
[5] LIAO Wen-Li, Bu Xiao-Ling, Jiang Wan-Yi, Liu Ji-Sheng. Influence of lipopolysaccharide on the expression of the Toll-like receptor gene BmToll9-2 in larval Bombyx mori [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 372-378.
[6] Thangaraj PRATHEEP, Neelamegam RAMESHKUMAR , Nagarajan KAYALVIZHI, Thangaiyan SUGANYA, Sivashanmugam KARTHIKEYAN, Muthukalingan KRISHNAN. Changes in lipophorin composition during late larval, pupal and adult stages of Bombyx mori (In English)
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 247-253.
[7] CHEN Shi-Liang, Xiao Sheng-Yan, Pan Qiu-Ling, Li Yong-Mou, Yang Rong-Gui, Gao Jian-Hua, Gao-Xiang, Xu-Jin-Shan. Infectivity and transmissibility of Nosema sp.CP isolated from Catopsilia pyranthe (Lepidoptera: Pieridae) in the domestic silkworm (Bombyx mori)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 155-162.
[8] YI Hua-Shan, Ma Xian-Ping, Pan Min-Hui, Lu Cheng. Eukaryotic expression and function analysis of Bombyx mori inhibitor of apoptosis protein (BmIAP) gene[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(1): 18-23.
[9] AN Huan-Ying, Wang Qin, Li Zhi, Dang Xiao-Qun, Zhou Ze-Yang, Wang Lin-Ling. Interaction between Nosema bombycis and proteins BmECH1 and BmGAPDH in Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(1): 45-52.
[10] YU Jie, XU Li, SHEN Zhong-Yuan, TANG Xu-Dong. cDNA cloning, expression anaylsis and subcellular localization of ubiquitin-like post modification protein NEDD8 in the silkworm,  Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 948-955.
[11] LI Xiao-Tong, SHI Lian-Gen. Progress in functions and underlying signal transduction mechanisms of neuropeptides and their receptors in the silkworm Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(7): 759-766.
[12] SHI Li-Li, GENG Tao, WU Ping, PAN Zhong-Hua, QIN Guang-Xing, GAO Kun, HOU Cheng-Xiang, GUO Xi-Jie. microRNA Novel-31* up-regulates the expression of hemocytin gene in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(7): 724-731.
[13] GUO Xing-Guo, CHEN Ying, XING Qiu-Ting, XING Wan-Jing, HAN Yun-Chao, QIAO Hui-Li. Expression and binding characterization of chemosensory protein CSP16 in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 613-621.
[14] ZHENG Zhi-Hua, XU Xiao-Xia, YU Jing, GAO Yan-Fu, ZHANG Yu-Qing, OUYANG Li-Na , JIN Feng-Liang. A peptidoglycan recognition protein (PxPGRP-SA) regulating the expression of antimicrobial peptides in Plutella xylostella (Lepidoptera: Plutellidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 489-499.
[15] YANG Li-Hong, DOU Wei, JIANG Hong-Bo, NIU Jin-Zhi, DING Tian-Bo, WANG Jin-Jun. Cloning and expression profiling of heat shock protein gene PcHsp90 in the citrus red mite, Panonychus citri (Acari: Tetranychidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(4): 411-420.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech