<em>Lactobacillus plantarum</em> promotes the growth and development of <em>Drosophila melanogaster</br> </em>
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (5): 544-552    DOI: 10.16380/j.kcxb.2017.05.006
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Lactobacillus plantarum promotes the growth and development of Drosophila melanogaster
LI Yu-Juan1, 2, SU Wan-Zhen2, HU Kun-Kun4, LI Peng-Cheng5, LIU Wei2,3,*, YAO Hong1,*
 (1. Department of Microbiology and Immunology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Medical Laboratory Science, Fenyang College Shanxi Medical University, Fenyang, Shanxi 032200, China; 3. Science and Technology Center, Fenyang College Shanxi Medical University, Fenyang, Shanxi 032200, China; 4. College of Life Science, Hubei University, Wuhan 430062, China; 5. Department of Respiratory Disease, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038, China)
 Download: PDF (3198 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to assay the effect of lactic acid bacteria on the developmental duration of fruit flies, and to further exploit their molecular mechanisms of growth stimulation of fruit flies. 【Methods】 Lactic acid bacteria were isolated from D. melanogaster using lactic acidic bacteria-selective medium MRS and identified by Gram staining, biochemical and 16S rRNA gene techniques. The symbiotic relationship was assayed by in vivo colonization and generation transfer experiments. The growth promoting effect of lactic acid bacteria on D. melanogaster was assessed in germ-free and gnotobiotic model. The expression levels of prothoracicotropic hormone gene (PTTH) and insulin-like receptor gene (InR) were examined using real-time PCR. The glucose concentration in haemolymph was examined using commercial kit with spetrometry. 【Results】 The isolated bacterium from D. melanogaster was identified as Lactobacillus plantarum strain FY1 (GenBank accesson no.: KY038178), which could colonize in the guts of flies with a mount of 104 CFU/gut and be transferred from the parental generation to their offspring. L. plantarum FY1 decreased the pH value of culture media to 5.2 in vitro, and shortened the egg-to-puparium duration of D. melanogaster from 20.0 d to 6.9 d and the egg-to-adult duration from 30.0 d to 10.7 d, respectively, resulting in 2-fold growth rate of flies compared with that of germ-free siblings. Real-time PCR result revealed that L. plantarum FY1 significantly stimulated the expression peak of PTTH gene in advance, while decreased the expression level of InR gene in D. melanogaster, and reduced the glucose concentration in haemolymph from 5.1 mg/mL to 2.7 mg/mL. 【Conclusion】 L. plantarum is a type of commensal bacteria of D. melanogaster, and might promote the growth and development of the host via insulin signal pathway.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
LI Yu-Juan
SU Wan-Zhen
HU Kun-Kun
LI Peng-Cheng
YAO Hong
Key wordsLactobacillus plantarum   Drosophila melanogaster   commensal bacteria   in vivo colonization   gut     
Cite this article:   
LI Yu-Juan,SU Wan-Zhen,HU Kun-Kun et al. Lactobacillus plantarum promotes the growth and development of Drosophila melanogaster
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 544-552.
http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.05.006      or     http://www.insect.org.cn/EN/Y2017/V60/I5/544
No references of article
[1] CHEN Bo-Sheng, LU Xing-Meng, SHAO Yong-Qi. Diversity of the gut microbiota in lepidopteran insects and their interaction with hosts[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 710-722.
[2] TIAN Xiao-Yan, SONG Fu-Ping, ZHANG Jie, LIU Rong-Mei, ZHANG Xing-Peng, DUAN Jiang-Yan, SHU Chang-Long. Diversity of gut bacteria in larval Protaetia brevitarsis (Coleoptera: Scarabaedia) fed on corn stalk[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 632-641.
[3] CHEN Da-Fu, Guo-Rui, Xiong Cui-Ling, Liang-Qin, Zheng Yan-Zhen, Xu Xi-Jian, Huang Zhi-Jian, Zhang Zhao-Nan, Zhang-Lu, Li Wen-Dong, Tong Xin-Yu, Xi Wei-Jun. Transcriptomic analysis of Ascosphaera apis stressing larval gut of Apis mellifera ligustica (Hyemenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 401-411.
[4] LIU Wei, Zhang-Ke, LI En-Hui, XU Hao-Zhe, Zhang-Ce. Escherichia coli alters the oviposition preference in Drosophila[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 173-179.
[5] LIU Xiao-Gai, YANG Ya-Jun, LIAO Qiu-Ju, XU Hong-Xing, LIU Ying-Hong, LU Zhong-Xian. Analysis of the bacterial community structure and diversity in the intestine of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 965-976.
[6] XU Yang, NAN Xiao-Ning , WEI Cong, HE Hong. Seasonal characteristics of gut bacterial communities associated with carpenter ant Camponotus japonicus (Hymenoptera: Formicidae)(In English[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 632-640.
[7] LI Kai-Xia, MA En-Bo, ZHANG Jian-Zhen, ZHAO Yi-Dan, ZHANG Xu-Bo. Combination of Gal80ts and Gal4 flexibly manipulates the expression levels of UAS transgenes in Drosophila[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 481-488.
[8] LIU Wei, LI Yu-Juan, LIU Xiao-Liang, ZHUO Ping, YAO Hong. Clostridium perfringens promotes the growth and development of Drosophila melanogaster  [J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 530-537.
[9] LI Wen-Hon, JIN Dao-Chao, LI Feng-Liang, CHENG Ying. Phenotypic fingerprints of bacterium Erwinia persicina from larval gut of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(4): 456-463.
[10] LU Yong-Peng, GU Ji-Hai, GAO Yi-Fu, TANG Ting, LIU Feng-Song, HUANG Da-Wei . Functional analysis of peptidoglycan recognition protein PGRP-SC in intestinal immunity of Musca domestica[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 269-277.
[11] ZHANG Mou, YANG Pu, ZHU Jia-Ying, YUAN Yuan, GUI Fu-Rong, GAO Xi, WU Guo-Xing. Analysis of the bacterial diversity in the intestine of larval Procecidochares utilis (Diptera: Trypetidae) based on 16S rDNA gene sequence[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 200-208.
[12] HE Qian-Yu, ZHANG Yuan-Xi. Molecular mechanisms regulating the binding capacity of juvenile hormone response region (JHRR) to nuclear proteins in Drosophila melanogaster[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(12): 1325-1331.
[13] ZHANG Chun-Hong, ZHANG Min-Jing, JIAO Wen-Juan, ZHU Qing-Zi, ZHU Zeng-Rong. Mechanisms of aging in Drosophila melanogaster[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(1): 102-110.
[14] LI Da-Peng, SHI Juan, LUO You-Qing. Mutualism between the Eurasian woodwasp, Sirex noctilio (Hymenoptera: Siricidae) and its fungal symbiont  Amylostereum areolatum (Russulales: Amylostereaceae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(9): 1019-1029.
[15] YAO Yun, LIN Xin-Da , WANG Bo. Effects of salbutamol on the genome stability, apoptosis and protein expression in Drosophila melanogaster[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(7): 699-705.
Supported by:Beijing Magtech