Number, distribution and morphology of cell bodies in the antennal lobe of <em>Plutella xylostella</em> (Lepidoptera: Plutellidae) adults
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ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 735-741    DOI: 10.16380/j.kcxb.2017.07.001
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Number, distribution and morphology of cell bodies in the antennal lobe of Plutella xylostella (Lepidoptera: Plutellidae) adults
YAN XiZhong1, XIE JiaoXin1, DENG CaiPing2, WU LanJun1, SUN XueJun1,3, HAO Chi1, *
(1. Agricultural College, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 2. Forestry College, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 3. Department of Experimental Oncology, University of Alberta, Cross Cancer Institute, Edmonton, Alberta T6G1Z2, Canada)
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Abstract 【Aim】 This study aims to investigate the number, distribution and morphology of cell bodies in the antennal lobe of adults of the diamondback moth, Plutella xylostella. 【Methods】 The nuclear staining and laser scanning confocal microscope were used to acquire the confocal image stacks of antennal lobe. The cell digital three-dimensional reconstructions were created by the Imaris software, and the volumes and surface areas of cell bodies were measured by using the statistic tool of the Imaris software. 【Results】 A lot of cell bodies are distributed in the medial cell cluster (MCCL), lateral cell cluster (LCCL) and anterior cell cluster (ACCL) of the antennal lobe of P. xylostella adults, and some irregular giant cells exist in the MCCL and LCCL. There are about 560 cell bodies in the antennal lobe in females, and 810 in males, more in males than in females. The morphology of cell bodies varies significantly, and three-dimensional reconstructions of cell bodies showed that most of them are nearly spherical. The surface area and volume of the smallest cell body are 96.7 μm2 and 76.9 μm3, and those of the nuclei are 42.5 μm2 and 24.1 μm3, respectively. The surface area and volume of the largest cell body are 389 μm2 and 514 μm3, and the surface area and volume of the nuclei are 265 μm2 and 261 μm3, respectively. 【Conclusion】 The characteristics of cell bodies in the antennal lobe of P. xylostella have been illustrated in this study, which will provide a basis for further understanding the anatomical structure and physiological function of the central nervous system.
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YAN Xi-Zhong
XIE Jiao-Xin
DENG Cai-Ping
WU Lan-Jun
SUN Xue-Jun
HAO Chi
Key wordsPlutella xylostella   brain   antennal lobe   cell body   three-dimensional model   laser scanning confocal microscope     
Cite this article:   
YAN Xi-Zhong,XIE Jiao-Xin,DENG Cai-Ping et al. Number, distribution and morphology of cell bodies in the antennal lobe of Plutella xylostella (Lepidoptera: Plutellidae) adults[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 735-741.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.07.001      or     http://www.insect.org.cn/EN/Y2017/V60/I7/735
 
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