Expression profiles of the cytochrome P450 gene <em>LmCYP6FD</em>3 in<em> Locusta migratoria</em> (Orthoptera: Acrididae) and its role in insecticide detoxification
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 742-749    DOI: 10.16380/j.kcxb.2017.07.002
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Expression profiles of the cytochrome P450 gene LmCYP6FD3 in Locusta migratoria (Orthoptera: Acrididae) and its role in insecticide detoxification
ZHU Wen-Ya1,2,3,4, WU Hai-Hua1,2,4, ZHANG Xue-Yao1,2,4, LIU Jiao1,2,4, ZHANG Jian-Zhen1,2,4,*, MA En-Bo1,2,4,*
(1. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; 2. College of Life Science, Shanxi University, Taiyuan 030006, China; 3. Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 4. Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030031, China)
 Download: PDF (2044 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 To study the molecular characterization and biological function of the cytochrome P450 gene LmCYP6FD3 from the migratory locust, Locusta migratoria. 【Methods】 The cytochrome P450 gene sequences were searched from the transcriptome database of L. migratoria, the cDNA fragment of the target gene was cloned using RT-PCR, and its expression patterns in various tissues (gastric caeca, foregut, midgut, hindgut, integument, testis, ovary, muscles, hemolymph, fat body and Malpighian tubules) of the 5th instar nymphs and different developmental stages (egg, 1st-5th instar nymph and adult) were analyzed using real-time quantitative PCR (RT-qPCR). The target gene was silenced by RNA interference (RNAi), the silence efficiency was evaluated by injecting dsRNA to the 2nd instar nymphs, and the susceptibility of the nymphs to three insecticides (malathion, carbaryl and deltamethrin) was assessed. 【Results】 The full-length cDNA of cytochrome P450 gene was cloned (GenBank accession no.: KT316378) and named LmCYP6FD3. Its length is 1 563 bp, encoding 521 amino acids. The expression level of LmCYP6FD3 was the highest in the Malpighian tubules of the 5th instar nymphs, moderate in the hindgut and fat body, and lower in other tissues. LmCYP6FD3 was expressed during the whole developmental period and had relatively higher expression level in the nymphal stage. Insecticide bioassay in combination with RNAi showed that the highest silence efficiency of LmCYP6FD3 was achieved at 24 h after RNAi, and the mortality of the 2nd instar nymphs of L. migratoria in the RNAi treatment group (injection with dsLmCYP6FD3) increased by 32% as compared with that of the control group (injection with dsGFP) after exposure to carbaryl by dipping method. 【Conclusion】 In this study, the full-length of cDNA sequence of LmCYP6FD3 was cloned. This gene has the highest expression level in the Malpighian tubules of L. migratoria and might play a significant role in the detoxification of carbaryl in this locust.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
ZHU Wen-Ya
WU Hai-Hua
LIU Jiao
ZHANG Jian-Zhen
MA 恩Bo
Key wordsCytochrome P450   Locusta migratoria   gene expression   RNA interference   insecticide     
Cite this article:   
ZHU Wen-Ya,WU Hai-Hua,ZHANG Xue-Yao et al. Expression profiles of the cytochrome P450 gene LmCYP6FD3 in Locusta migratoria (Orthoptera: Acrididae) and its role in insecticide detoxification[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 742-749.
URL:      or
No references of article
[1] ZHOU Hao, SHAO Ju-南, DI Yi-Fan, WU Guang-An, CHEN Hao, MEN Xing-Yuan, YU Yi, ZHENG Li. Toxicity and risk assessment of sulfoxaflor, spinetoram and their mixture to Bombus terrestris (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 809-816.
[2] LI Guo-Ping, FENG Hong-Qiang, HUANG Bo, JIN Yin-Li, TIAN Cai-Hong, HUANG Jian-Rong, QIU Feng. Assessment of toxicities of sixteen insecticides to the plant bug, Apolygus lucorum (Hemiptera: Miridae) by glass-vial and artificial diet bioassays (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 650-658.
[3] LIAO Wen-Li, Bu Xiao-Ling, Jiang Wan-Yi, Liu Ji-Sheng. Influence of lipopolysaccharide on the expression of the Toll-like receptor gene BmToll9-2 in larval Bombyx mori [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 372-378.
[4] CAI Qing-Ling, Lu Xue-Ying, Ma-Ji. Molecular cloning and expression profiling of a cold stress-related chitinase gene Mpcht19 in the desert beetle Microdera punctipennis (Coleoptera: Tenebrionidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 286-296.
[5] WANG Yan-Li, Yang Mei-Ling, Ma En-Bo, Zhang Jian-Zhen. Identification and analysis of the miRNAs targeting key genes involved in cuticle metabolism in Locusta migratoria (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 309-317.
[6] ZHANG Yuan-Chen, Liu Xiang-Dong. Cloning and expression analysis of lysozyme gene AgLYS in the cotton-melon aphid,Aphis gossypii (Hemiptera: Aphididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 127-135.
[7] HUANG Ling, SUN Liang-Zhen, WANG Yong, RU Yu-Tao, Muhammad IRFAN, JIANG Yi-Ren, Shi-Sheng-Lin , YANG Rui-Sheng, LI Xi-Sheng, QIN Li. Changes in the expression of trehalose-6-phosphate synthase gene in Antheraea pernyi (Lepidoptera: Saturniidae) during pupal diapause termination[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 938-947.
[8] LIU Ji-Sheng, ZHU Wen-Hui, LIAO Wen-Li, BU Xiao-Ling, JIANG Wan-Yi. dsRNA delivery methods for RNA interference in insects[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 682-691.
[9] LI Kai-Xia, MA En-Bo, ZHANG Jian-Zhen, ZHAO Yi-Dan, ZHANG Xu-Bo. Combination of Gal80ts and Gal4 flexibly manipulates the expression levels of UAS transgenes in Drosophila[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 481-488.
[10] CHENG Xiao-Juan, CAI Li-Jun, ZHENG Li-Shuang, QIN Jiang-Mei, HUANG Yu-Ping, YOU Min-Sheng . Cloning, expression profiling and binding characterization of the OBP2 gene in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(4): 365-376.
[11] LIU Yang, GAO He, LI Xue-Jiao, WU Jun-Xiang, CHENG Wei-Ning, ZHU-SALZMAN Ke-Yan. Changes in sorbitol content and the expression level of sorbitol dehydrogenase gene in Sitodiplosis mosellana (Diptera: Cecidomyiidae) larvae at different diapause stages[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 119-126.
[12] ZHANG Yang, LIU Ji, YANG Jie, CUI Qing-Qing, SHEN Zhong-Yuan, WU Jin-Mei. Peptidoglycan recognition protein L1 is involved in the Imd pathway in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 164-171.
[13] YU Rong-Rong, DING Guo-Wei, YANG Mei-Ling, MA 恩Bo, ZHANG Jian-Zhen. Silence efficiency of mRNA and protein expression of Knickkopf in Locusta migratoria (Orthoptera: Acrididae) by dsRNA injection[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(12): 1395-1340.
[14] ZHANG Xiao-Lei, LIAO Xun, MAO Kai-Kai, WAN Hu, LU Peng, LI Jian-Hong. Resistance monitoring of the field populations of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) to common insecticides in rice production areas of Hubei Province, central China[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1222-1231.
[15] LIU Yi-Cong, WANG Ling, ZHANG You-Jun, XIE Wen, WU Qing-Jun, WANG Shao-Li. Abamectin resistance and expression of resistance-related genes in field populations of Tetranychus urticae (Acari: Tetranychidae) in China[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1199-1205.
Supported by:Beijing Magtech