Proteomic analysis of ovaries of queen bees (<em>Apis mellifera</em>) developed from larvae grafted at different instars (<em>In English</em>)
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ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 760-771    DOI: 10.16380/j.kcxb.2017.07.004
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Proteomic analysis of ovaries of queen bees (Apis mellifera) developed from larvae grafted at different instars (In English)
PANG Qian1, SHEN Fang2, WANG Kang1, ZHANG Wen-Wen1, YIN Ling3, JI Ting1,*
(1. College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; 2. Fuyang Animal Husbandry and Veterinary Burean, Fuyang, Anhui 236000, China; 3. Jiangsu AgriAnimal Husbandry Vocational College, Taizhou, Jiangsu 225300, China)
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Abstract 【Aim】 To explore the molecular regulation mechanism of grafted larvae on ovarian development of queens in the Western honey bee, Apis mellifera. 【Methods】 The isobaric tags for relative and absolute quantitation (iTRAQ) method was used to quantify the proteins in the ovaries of queens reared from the larvae grafted at different instars and screen the differentially expressed proteins (DEPs). The results were validated by Western blotting. 【Results】 A total of 452 966 spectra were obtained and 3 642 proteins were identified from ovaries of A. mellifera queens. Gene Ontology (GO) analysis revealed that the DEPs in the ovaries of queens reared from the larvae grafted at different instars are associated with cell metabolism, cell division, and protein biosynthesis. The DEPs in the ovaries of queens reared from the larvae grafted between the 1st and 2nd instars are enriched in such pathways as carbohydrate metabolism, lipid metabolism, and exogenous degradation, while those reared from the larvae grafted between the 1st and 3rd instars mainly enriched in developmental pathways, ribosome pathways, and lysosome metabolism. The Western blotting results of the two differentially expressed storage proteins hexamerin 110 and hexamerin 70b showed that their expression levels decreased with the grafted larval instar. 【Conclusion】 The identification of differentially expressed proteins in the ovaries of A. mellifera queens reared from the larvae grafted at different instars provides a foundation for the regulatory mechanism of queen reproductive development and caste differentiation.  
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YIN Ling
JI Ting
Key wordsApis mellifera; grafted larva   instar   ovary   queen   proteomics   differentially expressed proteins     
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PANG Qian,SHEN Fang,WANG Kang et al. Proteomic analysis of ovaries of queen bees (Apis mellifera) developed from larvae grafted at different instars (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 760-771.
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