Identification of bacterial proteins in saliva of adults of the brown planthopper, <em>Nilaparvata lugens</em> (Hemiptera: Delphacidae)
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ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 772-781    DOI: 10.16380/j.kcxb.2017.07.005
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Identification of bacterial proteins in saliva of adults of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
MIAO Yu-Tong, DENG Yao, LIU Yu-Di*, HOU Mao-Lin
(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
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Abstract 【Aim】 To understand the bacteria species in saliva of adults of the brown planthopper, Nilaparvata lugens, by identifying the bacterial proteins in their saliva. 【Methods】 Saliva of N. lugens adults was collected by stretching two layers of Parafilm with sucrose diet. Protein solution was subjected to electrophoresis after concentration by ultrafiltration. Protein identification was conducted by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) analysis, and the spectra were searched against the Uniprot database of bacterial protein to identify the bacterial proteins in saliva. 【Results】 Thirty five proteins of twenty-two species of bacteria, mainly related to energy metabolism, protein folding and synthesis and amino acid metabolism, were identified in saliva of N. lugens adults. These bacteria belong to Proteobacteria, Actinobacteria and Firmicutes, and most are from Proteobacteria. 【Conclusion】 The endosymbiont bacteria in N. lugens, whose proteins were identified, may play an important role in the life history of this insect.
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MIAO Yu-Tong
DENG Yao
LIU Yu-Di
HOU Mao-Lin
Key wordsNilaparvata lugens   saliva   bacterial protein   endosymbiont bacteria   LC-ESI-MS/MS     
Cite this article:   
MIAO Yu-Tong,DENG Yao,LIU Yu-Di et al. Identification of bacterial proteins in saliva of adults of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 772-781.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.07.005      or     http://www.insect.org.cn/EN/Y2017/V60/I7/772
 
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