Effects of Vip3Aa11 protein on<em> Ostrinia furnacalis</em> (Lepidoptera: Pyralidae) and its parasitoid <em>Macrocentrus cingulum</em> (Hymenoptera: Braconidae)
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 817-824    DOI: 10.16380/j.kcxb.2017.07.010
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Effects of Vip3Aa11 protein on Ostrinia furnacalis (Lepidoptera: Pyralidae) and its parasitoid Macrocentrus cingulum (Hymenoptera: Braconidae)
HAO Jie1,2, WANG Zhen-Ying2, WANG Qin-Ying1, BAI Shu-Xiong2, ZHANG Tian-Tao2, NANGONG Zi-Yan1, *, HE Kang-Lai2,*
(1. College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071000, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
 Download: PDF (910 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 The aim of this study is to clarify the effects of Vip3Aa11 toxin on the Asian corn borer, Ostrinia furnacalis, and its parasitoid Macrocentrus cingulum. 【Methods】 The indirect effects of Vip3Aa11 protein on M. cingulum were assessed by the bioassay with the artificial diet containing 6 μg/g azadirachtin used as the positive control. The mortality and parasitism of O. furnacalis larvae parasitized by M. cingulum were recorded at 10 d after feeding with a diet containing 100 μg/g Vip3Aa11 protein, and the cocoon weight of M. cingulum and the emergence number per O. furnacalis larva after pupation and emergence of M. cingulum were investigated. The direct effects of Vip3Aa11 protein on M. cingulum were assessed by the bioassay with 20% honey solution containing 100 μg/g azadirachtin used as the positive control. The mortality of M. cingulum adults daily after feeding with 20% honey solution containing 100 μg/g Vip3Aa11 protein, and the mortality and parasitism rate of O. furnacalis larvae at 10 d after being parasitized by M. cingulum adults from different treatments were recorded. The cocoon weight of M. cingulum and emergence number per O. furnacalis larva of next generation of M. cingulum were recorded after pupation and emergence. 【Results】 The bioassay results showed that the average mortality and average inhibition rate of body weight of O. furnacalis larvae were 50.7% and 77.1%, respectively, at 7 d after exposure to 100 μg/g Vip3Aa11 protein. In the bioassay of indirect effects, the mortality of O. furnacalis larvae significantly increased when they were fed with the artificial diet containing Vip3Aa11 protein after parasitized by M. cingulum, and the cocoon weight and the number of M. cingulum progenies produced per host decreased; however, the toxin had no effect on the parasitism rate and longevity of M. cingulum adults. In the bioassay of direct effects, the longevity and parasitism rate of M. cingulum adults, the mortality of O. furnacalis larvae, and the cocoon weight, the number of progenies produced per host and the adult longevity of next generation of M. cingulum were not affected when M. cingulum adults were fed with 20% honey solution containing 100 μg/g Vip3Aa11 protein. 【Conclusion】 This study assessed the potential effects of Vip3Aa11 toxin on M. cingulum from the indirect and direct aspects through bioassay system. The results demonstrate that M. cingulum is insensitive to Vip3Aa11 protein at the concentrations exceeding those encountered in Bt crop fields. Vip3Aa11 protein has no direct adverse effects on M. cingulum, and the indirect detrimental effects detected in the bioassay may be due to poor host quality.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
HAO Jie
WANG Zhen-Ying
WANG Qin-Ying-1 Bai-Shu-Xiong
ZHANG Tian-Tao
南Gong-Zi-Yan
HE Kang-Lai
Key wordsMacrocentrus cingulum   Ostrinia furnacalis   insecticidal protein   Vip3Aa11   bioassay   parasitism     
Cite this article:   
HAO Jie,WANG Zhen-Ying,WANG Qin-Ying-1 Bai-Shu-Xiong et al. Effects of Vip3Aa11 protein on Ostrinia furnacalis (Lepidoptera: Pyralidae) and its parasitoid Macrocentrus cingulum (Hymenoptera: Braconidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 817-824.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.07.010      or     http://www.insect.org.cn/EN/Y2017/V60/I7/817
 
No references of article
[1] ZHANG Xu, WU Peng-Xiang, MA Bao-Xu, ZHANG Yan-Zhou. Psyllaephagus arenarius (Hymenoptera: Encyrtidae), a newly recorded parasitoid of Bactericera gobica (Hemiptera: Psyllidae) in China[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 842-846.
[2] WU Peng-Xiang, Ma Bao-Xu, Xu-Jing, Zhang-Rong, He-Jia, Zhang Run-Zhi. Parasitic biological characteristics of Psyllaephagus arenarius (Hymenoptera:Encyrtidae) on Paratrioza sinica (Hemiptera: Psyllidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 431-440.
[3] LIN Zhe-Guang, QIN Yao, LI LI, WANG Shuai, ZHENG Huo-Qing, HU Fu-Liang. Synergistic effects of Varroa destructor and Deformed wing virus on honey bee health[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(7): 775-784.
[4] XU Yu-Hang, ZHOU Jin-Cheng, LI Bao-Ping, MENG Ling. Survival and developmental performance of Meteorus pulchricornis in intrinsic competition with Microplitis mediator within the shared host Mythimna separata(Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 322-327.
[5] LUO Jian-Feng, LIU Huan, GUO Zi-Jun, WANG Guo-Cai, LING Bing. Inhibition of cellular proliferation and induction of necrosis in Ofh cells of Ostrinina furnacalis (Lepidoptera: Pyralidae) by momordicin I and charantin B[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(10): 1093-1102.
[6] SONG Jing, HUANG Jing, WANG Lei-Ying, LI Yuan-Xi. Effects of rearing host on parasitization behavior and capacity of Trichogramma japonicum (Hymenoptera: Trichogrammatidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(7): 783-790.
[7] CAO Hong-Mei, ZHENG Li-Xia, WEI Hong-Yi. Effects of heavy metal Ni2+ on the development and reproductive behavior of Ostrinia furnacalis (Lepidoptera: Pyralidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(6): 650-657.
[8] CAO Xi, ZHU Chun-Ya, ZHANG Mao-Xin, LING Bing. Bioactivity of momordicin I against Ostrinia furnacalis (Lepidoptera: Pyralidae) and its effects on the metabolizing enzyme activities in larval bodies of the moth[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(6): 625-633.
[9] YU Guo-Yue. First discovery of the honeysuckle whitefly, Aleyrodes lonicerae Walker (Hemiptera: Aleyrodidae) in China[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(12): 1368-1372.
[10] WANG Jing, ZHANG Bo, HOU Xiang, CHEN Xiao-Ning, HAN Ning, CHANG Gang. Seed production and seed size and their relationship with insect infestation in Quercus glandulifera and Quercus aliena var. acuteserrata in the south slopes of the Qinling Mountains[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(12): 1307-1314.
[11] SHEN Dong-Xu, CHU Yuan, HONG Fang, LIU Yang, WANG Gui-Rong , ANChun-Ju. Molecular cloning and functional analysis of C-type lectin 6 (CTL6) in Ostrinia furnacalis (Lepidoptera: Crambidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(11): 1177-1183.
[12] ZHANG Ping, MENG Ling, LI Bao-Ping. Parasitizing, developmental and reproductive performances of Meteorus pulchricornis (Hymenoptera: Braconidae) in relation to host age at oviposition[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(9): 1032-1036.
[13] ZHAO Tong-Wei ZHANG Bing, Lü Wen-Jing, ZHAI Hui-Feng, WU Tao-Yan, ZHANG Shi-Yang, TANG Xiao-Wei, FENG Cong-Jing. Prokaryotic expression and characterization of prophenoloxidase from the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(7): 798-805.
[14] ZHU Xiao-Xiang, CANG Tao, WANG Yan-Hua, WU Chang-Xing, ZHAO Xue-Ping, WANG Qiang. Acute toxicity and risk assessment of triazole fungicides to adult wasps of threeTrichogramma species (Hymenoptera: Trichogrammidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(6): 688-695.
[15] SUN Li-Ying, YANG Nian-Wan, WANG Jin-Jun, WAN Fang-Hao. Reproductive traits of autoparasitoids and their influences on biological control[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(3): 365-372.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech