Comparative morphology and ultrastructure of Malpighian tubules of the mute cicada Karenia caelatata and the coleopterous spittlebug Lepyronia coleoptrata </br> (Hemiptera: Cicadomorpha) (<em>In English</em>)
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (7): 825-834    DOI: 10.16380/j.kcxb.2017.07.011
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Comparative morphology and ultrastructure of Malpighian tubules of the mute cicada Karenia caelatata and the coleopterous spittlebug Lepyronia coleoptrata
(Hemiptera: Cicadomorpha) (In English)
ZHONG Hai-Ying1, 2, ZHANG Ya-Lin1, WEI Cong1,*
(1. Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
 Download: PDF (5612 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 In order to better understand the morphology and function of Malpighian tubules of the Cicadomorpha, comparative morphology and ultrastructure of the mute cicada Karenia caelatata Distant and the coleopterous spittlebug Lepyronia coleoptrata (Linnaeus) were investigated. 【Methods】 The Malpighian tubules of adult K. caelatata and L. coleoptrata were observed using both light and transmission electron microscopies. 【Results】 The results show that except the anterior-most segment that takes part in the formation of the filter chamber, each tubule outside the filter chamber of both K. caelatata and L. coleoptrata is morphologically differentiated into four regions, i.e., intermediate duct, proximal segment, distal segment, and terminal segment. Apical parts of the terminal segments in K. caelatata are attached to the ileum, with the posterior-most ends united in pairs and enveloped by a connetcive tissue shortly before arriving at the rectum, whereas the corresponding parts in L. coleoptrata terminate as separated rods and closely attached to the rectum. Ultrastructurally, cells of the tubules in both species possess basal infoldings and apical microvilli. Cells of the proximal segment in K. caelatata possess secretory vesicles, secretory granules, rough endoplasmic reticulum and urospherites. In L. coleoptrata, cells of the proximal segment possess abundant secretory vesicles, and cells of the distal segment contain oval vesicles which appear to fuse with the basal plasma membrane. The microorganisms observed in the proximal and distal segments of K. caelatata are possibly symbiotic bacteria, while those resided in the intermediate duct of L. coleoptrata are likely to be pathogenic. 【Conclusion】 The similarities and differences in morphological characteristics and ultrastructure of Malpighian tubules in the two Cicadomorpha representatives, K. caelatata and L. coleoptrata, not only support the sister relationship of Cicadoidea and Cercopoidea, but also improve our understanding of the functional differentiation of Malpighian tubules in the Cicadomorpha. The discovery of microorganisms in the Malpighian tubules of the two species is informative to the future study of coevolution between the Cicadomorpha and related bacteria and to the biological control of related pests.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
ZHONG Hai-Ying
WEI Cong
Key wordsAuchenorrhyncha; Karenia caelatata   Lepyronia coleoptrata   excretory system   symbiotic bacteria   transmission electron microscopy   light microscopy     
Cite this article:   
ZHONG Hai-Ying,ZHANG Ya-Lin,WEI Cong. Comparative morphology and ultrastructure of Malpighian tubules of the mute cicada Karenia caelatata and the coleopterous spittlebug Lepyronia coleoptrata
(Hemiptera: Cicadomorpha) (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 825-834.
URL:      or
No references of article
[1] XIE Jiao-Xin, WU Lan-Jun, SUN Xue-Jun, YAN Xi-Zhong, HAO Chi. Structural observation and analysis of the proboscis formed by the linkage of the galeae in Plutella xylostella (Lepidoptera: Plutellidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 835-841.
[2] WEI Jing, WANG Xiao-Wei, LIU Shu-Sheng. Research progress in geminivirus transmission by whiteflies (Hemiptera: Aleyrodidae) and the underlying molecular mechanisms[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(4): 445-453.
[3] TAN JiangLi1,*, LI JunLin1, XING LianXi1, CHEN XueXin2. Morphology of seminal vesicle and sperm of the male Polistes chinensis antennalis (Hymenoptera: Vespidae)[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(9): 989-995.
[4] WANG YueMing1, 2, LIU XiaoYu1, HUANG LiNian2, SUN Xin3, LIU ZhiGang1,*. Ultrastructure of the reproductive system in Dermatophagoides farinae (Acariformes: Pyroglyphidae) observed with transmission electron microscopy[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(8): 960-964.
[5] YU Jie, CHI De-Fu, LI Xiao-Can, YU Jia. Changes in external morphology and integument ultrastructure of the 5th instar larvae of Lymantria dispar (Lepidoptera: Lymantridae) treated by 20hydroxyecdysone[J]. ACTA ENTOMOLOGICA SINICA, 2012, 55(4): 386-394.
[6] YANG Mei-Hong, ZHANG Jin-Tong, FAN Li-Hua, LIU Hong-Xia, LUO You-Qing, ZONG Shi-Xiang, CAO Chuan-Jian. Ultrastructural observation of the sex pheromone communication system in Holcocerus vicarius (Walker) (Lepidoptera: Cossidae)[J]. ACTA ENTOMOLOGICA SINICA, 2011, 54(5): 522-530.
[7] . Ultrastructure of olfactory sensilla on the antenna of Proagopertha lucidula (Coleoptera: Scarabaeidae)[J]. ACTA ENTOMOLOGICA SINICA, 2009, 52(1): 39-45.
[8] SUN Fan, HU Ji-Hua, WANG Guang-Li, PENG Lu. Ultrastructure of olfactory sensilla of Holotrichia diomphalia Bates(Coleoptera: Melolonthidae)[J]. ACTA ENTOMOLOGICA SINICA, 2007, 50(7): 675-681.
[9] . Histopathological changes in the midgut of the rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) larvae feeding on transgenic Bt rice[J]. ACTA ENTOMOLOGICA SINICA, 2007, 50(10): 1070-1076.
[10] WANG Yin, LEI Zhong-Ren, ZHANG Qing-Wen, WEN Jin-Zeng. Observation of infection process of Metarhizium anisopliae on Plutella xylostella larvae with transmission electron microscopy[J]. ACTA ENTOMOLOGICA SINICA, 2006, 49(6): 1042-1045.
[11] RUAN Yong-Ming, LIU Shu-Sheng. Detection and phylogenetic analysis of prokaryotic endosymbionts in Bemisia tabaci[J]. ACTA ENTOMOLOGICA SINICA, 2005, 48(6): 859-865.
[12] GONG Peng, SHEN Zuo-Rui, LI ZhiH-ong. Wolbachia endosymbionts and their manipulation of reproduction of arthropod hosts[J]. ACTA ENTOMOLOGICA SINICA, 2002, 45(2): 241-252.
Supported by:Beijing Magtech