Carboxylesterase LsCarE1 mediates insecticide resistance in <em>Laodelphax striatellus</em> (Hemiptera: Delphacidae)  
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (9): 1006-1012    DOI: 10.16380/j.kcxb.2017.09.004
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Carboxylesterase LsCarE1 mediates insecticide resistance in Laodelphax striatellus (Hemiptera: Delphacidae)  
WANG Rui1, ZHANG Hao-Miao1, DENG Lei1, XIAO Qian-Qian1, WANG Qiu-Xia1, LIU Guang-Na2, HAN Zhao-Jun1, WU Min1, *
(1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 2. Jilin Agricultural Science and Technology University, Jilin 132101, China)
 Download: PDF (1289 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 In our previous studies, the activities of total esterases were found to be significantly increased in the chlorpyrifos, imidacloprid and deltamethrin resistant strains of the small brown planthopper, Laodelphax striatellus. The aim of this study is to identify the carboxylesterase gene causing this increase and to verify its roles in insecticide resistance. 【Methods】 The expression levels of carboxylesterase genes in female adults of the resistant and susceptible strains of L. striatellus were detected by transcriptome sequencing and RT-qPCR. Microinjection was used to establish a transgenic Drosophila melanogaster strain overexpressing the target gene. Topical application method was used to investigate the responses of transgenic D. melanogaster overexpressing the target gene to chlorpyrifos, imidacloprid and deltamethrin, respectively. 【Results】 The expression level of LsCarE1 (GenBank accession no.: HM600723) was upregulated in female adults of the chlorpyrifos, imidacloprid and deltamethrin resistant strains of L. striatellus. Based on the sequence of LsCarE1, codon optimization was performed, and the open reading frame of LsCarE1 was cloned into the transgenic vector pUAST-attB. The transgenic homozygous strain UAS-LsCarE1Optimized was successfully established and then genetically crossed with the GAL4 driver line to obtain a da>LsCarE1Optimized strain that exhibited the overexpression of LsCarE1. The expression level of LsCarE1 in the da>LsCarE1Optimized strain was 257.3 times as high as that in the reference strain UAS-LsCarE1Optimized. The expression of LsCarE1 was not detected in the reference strain da>gal4. Furthermore, the expression levels of the endogenous esterase homologous genes in D. melanogaster were significantly lower than that of LsCarE1. The bioassay results indicated that as compared with the control strain da>gal4, the overexpression strain da>LsCarE1Optimized  showed increased tolerance to chlorpyrifos and imidacloprid by 4.19- and 2.46-fold, respectively, but showed no significantly increased tolerance to deltamethrin. 【Conclusion】 The overexpression of LsCarE1 is related to the increased resistance to chlorpyrifos and imidacloprid in L. striatellus.  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WANG Rui
ZHANG Hao-Miao
DENG Lei
XIAO Qian-Qian
WANG Qiu-Xia
LIU Guang-Na
HAN Zhao-Jun
WU Min
Key wordsLaodelphax striatellus; metabolic resistance   carboxylesterase   transgenic Drosophila   overexpression     
Cite this article:   
WANG Rui,ZHANG Hao-Miao,DENG Lei et al. Carboxylesterase LsCarE1 mediates insecticide resistance in Laodelphax striatellus (Hemiptera: Delphacidae)  [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(9): 1006-1012.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.09.004      or     http://www.insect.org.cn/EN/Y2017/V60/I9/1006
 
No references of article
[1] XU Lu, Zhao Chun-Qing, Xu De-Jin, Xu Guang-Chun, Xu Xiao-Long, Zhang Ya-Nan, Han Zhao-Jun, Gu Zhong-Yan. Molecular cloning and sequence analysis of the estrogen-related receptor (ERR) gene in the small brown planthopper, Laodelphax striatellus (Delphacidae: Hemiptera) and its expression profiles under the stress of sulfoxaflor[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 264-273.
[2] LIU Jia, ZHOU Yong, ZHU Hang, MA Hai-Hao, DENG Xi-Le, ZHOU Xiao-Mao, BAI Lian-Yang. Monitoring of insecticide resistance in Spodoptera litura (Lepidoptera: Noctuidae) and the effect of indoxacarb on its detoxification enzymes[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1254-1262.
[3] LI Yang, SHI Xue-Yan, GAO Xi-Wu. In vivo effects of piperonyl butoxide on the activities of 7-ethoxycoumarin O-deethylase and carboxylesterase in larval Chilo suppressalis (Lepidoptera: Pyralidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1159-1165.
[4] LU Yan-Hui, GAO Xi-Wu. Inhibiting effects of common insecticides on carboxylesterase activity in Sitobion avenae and Rhopalosiphum padi (Hemiptera: Aphididae) and their synergism to beta-cypermethrin[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1151-1158.
[5] REN Na-Na, XIE Miao, YOU Yan-Chun,LI Jian-Yu, CHEN Wei-Jun , CHENG Xue-Min, YOU Min-Sheng. Fipronil-resistance mediated by carboxylesterases in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(3): 288-296.
[6] CAO Wei, MA Zheng, YU Xiao-Ping. Isolation and sensitivity to fungicides of the yeast-like symbiont Pichia anomala (Hemiascomycetes: Saccharomycetaceae) from Laodelphax striatellus (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(3): 271-280.
[7] ZHANG Qing, LU Ming-Xing, ZHU Shu-De. Cloning, sequence analysis and expression profiling of heat shock protein 90 gene in the small brown plant hopper, Laodelphax striatellus (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(7): 777-786.
[8] AN Zhi-Fang, YU Ju-Long, PENG Juan, ZHANG Chao, LIU Xiang-Dong. Differentiation of wing forms in pure macropterous and brachypterous lineages is less subject to photoperiod in rice planthoppers (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(11): 1306-1314.
[9] ZHAO Dong-Xiao, ZHANG Yan-Kai, CHEN Han, HONG Xiao-Yue. Effects of transinfection of Wolbachia from Laodelphax striatellus (Hemiptera: Delphacidae) on reproductive fitness and adult longevity of Tetranychus phaselus (Acari: Tetranychidae)[J]. ACTA ENTOMOLOGICA SINICA, 2014, 57(1): 25-35.
[10] WANG Li-Hua, ZHUANG Ze-Jin, LI Yong-Teng, FANG Ji-Chao. Biological characteristics and mating advantage of the red-eye mutant of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(8): 878-883.
[11] . Effect of two single mutations on malathion degradation by insect carboxylesterases[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(5): 566-569.
[12] MI Zhi, RUAN Cheng-Long, LI Jiao-Rong, FU Qiao-Juan, WU Jing-Jie, SENDEGEYA Parfait, ZHU Yong . Influence of fluoride on activities of carboxylesterases and esterases in the larval midgut of the fluoride-resistant and susceptible strains of Bombyx mori
[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(5): 494-498.
[13] YUE Qiu-Juan, YAO Shu-Min, LIU Yang-Yang, LIU Shi-Juan, CUI Feng . Screening of an insecticide susceptible strain of Culex pipiens quinquefasciatus (Diptera: Culicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(4): 379-384.
[14] WANG Liu-Feng, FU Shu, XIAO Liang, CHEN Chao, XUE Fang-Sen. Life history, reproduction and overwintering biology of the small brown planthopper, Laodelphax striatellus[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(12): 1430-1439.
[15] LIU Chang-Li, LU Li-Xia, XU Yan-Li, YANG Peng-Cheng, CUI Feng. Transcriptomic analysis of three detoxification enzyme families in the salivary glands of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2013, 56(12): 1509-1515.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech