Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morus alba branches induced by jasmonic acid
WANG Wei1, LI Ji-Quan1,2,*, CUI Jian-Zhou1,2, LI Zeng-Liang3, ZHANG Qing1, TIAN Hong-Yu1, TANG Zhe1
(1. College of Forestry, Agricultural University of Hebei, Baoding, Hebei 071000, China; 2. Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei Province, Baoding, Hebei 071000, China; 3. Center for Quality Supervision and Test of Forestry Fruit, Mullbery and Flower of Hebei Province, Shijiazhuang 050081, China)
Abstract 【Aim】 This study aims to clarify the electroantennographic and olfactory responses of female wasps of the eulophid Aprostocetus prolixus to volatiles of Morus alba branches induced by jasmonic acid (JA), so as to reveal the chemical mechanisms of indirect resistance in M. alba branches against Apriona germari. 【Methods】 The volatiles from M. alba branches treated with JA were collected by using the dynamic headspace volatile collection method. The electroantennographically active compounds for female wasps of A. prolixus were screened and indentified by coupled gas chromatography-electroantennographic detector (GC-EAD) and GC/MS, respectively, and the differences in emission rates of the EAD-active components between the JAtreated M. alba branches and the water-treated control branches were also compared. Furthermore, the volatile compounds attractive to female A. prolixus were determined by Y-tube olfactometer. 【Results】 The results demonstrated that Z-3-hexenol, β-terpinene, β-phallandrene, perillene, methyl salicylate and other three unknown chemicals from M. alba branches treated with JA elicited significant GC-EAD responses in female wasps of A. prolixus. The emission rates of β-phallandrene, perillene and methyl salicylate from the JA-treated M. alba branches were significantly higher than those from the control branches. Furthermore, β-phallandrene and perillene were detected only in the volatiles of the JA-treated plants. However, there was no significant difference in the emission rate of Z-3-hexenol between the JA-treated plants and the control plants. And 0.1% β-phallandrene, 0.1% perillene and 0.01% and 0.001% methyl salicylate were significantly attractive to female wasps of A. prolixus. 【Conclusion】 The results suggest that highly elevated emission of β-phallandrene, perillene and methyl salicylate from the JA-treated M. alba branches is one of the important mechanisms of the JA-induced indirect resistance in M. alba tree against A. germari.
WANG Wei,LI Ji-Quan,CUI Jian-Zhou et al. Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morus alba branches induced by jasmonic acid
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(9): 1013-1020.