Quantitative evaluation of the paralytic activity of an ENF peptide in<em> Bombyx mori</em> (<em>In English</em>)
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ACTA ENTOMOLOGICA SINICA  2017, Vol. 60 Issue (10): 1114-1119    DOI: 10.16380/j.kcxb.2017.10.002
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Quantitative evaluation of the paralytic activity of an ENF peptide in Bombyx mori (In English)
WANG Fei*, DONG Shi-Feng, SONG Liang, HU Jie, XIA Qing-You
(State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China)
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Abstract 【Aim】 A Glu-Asn-Phe (ENF) peptide, paralytic peptide (PP) identified in several lepidopteran hemolymph induces rapid and rigid paralysis defined by a tonic contraction when injected into larvae. This study aims to determine the optimal amount and maximum contraction that PP induces in live larvae of the silkworm, Bombyx mori, and to examine the possible change of other physiological index, such as pulse velocity and ion concentration which might accompany the contraction. 【Methods】 The intensity of body contraction and pulse velocity were monitored after injection of various amounts of PP into the body of the 5th instar larvae. And the ion concentrations in hemolymph, fat body and digestive tube were measured by atomic absorption spectroscopy. 【Results】 PP at the concentration of 50 ng/g animal triggered the most potent contraction with no lethal effect. The maximum body contraction was seen between 4 and 5 min after injection. Digestive tube distortion was found to accompany body wall contraction, and pulse velocity decreased when the body reached the maximum contraction. In addition, extracellular Ca2+ was required for the contraction and PP also stimulated a sharp decrease then a slow recovery of Cl- concentration in hemolymph. 【Conclusion】 The paralytic activity of PP not only affects the body wall, but also digestive tube and dorsal vessel of silkworm larvae, and is associated with disruption of Cl- homeostasis. Our results will provide reference for studying the physiological role of PP in an animal model.
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WANG Fei
DONG Shi-Feng
SONG Liang
HU Jie
XIA Qing-You
Key wordsBombyx mori; paralytic peptide   contraction   ion concentration   pulse velocity     
Cite this article:   
WANG Fei,DONG Shi-Feng,SONG Liang et al. Quantitative evaluation of the paralytic activity of an ENF peptide in Bombyx mori (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1114-1119.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2017.10.002      or     http://www.insect.org.cn/EN/Y2017/V60/I10/1114
 
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