Mating and oviposition behaviors of Pagiophloeus tsushimanus (Coleoptera: Curculionidae)
ZHANG Cong-Cong1,2, WANG Meng1,2, SU Peng3, FAN Bin-Qi4, WANG Yan4, HAO De-Jun1,2,*
(1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Forestry, Nanjing Forestry University, Nanjing, China; 3. Songjiang District Forest Station of Shanghai, Shanghai 310117, China; 4. Shanghai Forestry Station, Shanghai 200072, China)
Abstract 【Aim】 Pagiophloeus tsushimanus is a newly recorded species in China, which injures Cinnamomum camphora. The study aims to learn more the behavioral characteristics of P. tsushimanus by investigating its adult mating and oviposition behaviors. 【Methods】 Mating and oviposition behaviors of P. tsushimanus adults was recorded by direct observation and video recording in the laboratory and analyzed. 【Results】 A complete mating of P. tsushimanus involves three stages, i.e., pre-mating, mating and post-mating, exhibiting the behavioral characteristics of encountering pair-bonding, insemination and post-copulatory guarding. An entire mating procedure lasts about 672.67±156.53 min. The duration of the mounting, insemination, and post-copulatory guarding behaviors was 103.94±20.61 min, 333.83±94.15 min, and 234.9±41.79 min, respectively. The whole process of oviposition can be divided into three stages, i.e., burrowing oviposition scar, egg deposition and covering oviposition scars, including 52.43±4.93 min for making an oviposition scar, 2.47±0.14 min for laying an egg, and 29.09±6.74 min for covering an oviposition scar. There are multiple mating behaviors in the whole mating process for the female and male adults of P. tsushimanus, and mating behavior occurs day and night. The major peak of mating took place from 12:00 to 14:00, with a minor peak from 6: 00 to 8: 00. The female adults of P. tsushimanus display obvious circadian rhythms in oviposition behaviors. The peak of oviposition occurred from 18: 00 to 20: 00, with a minor peak from 8: 00 to 10: 00. 【Conclusion】 The results contribute to elucidate the reproductive process of P. tsushimanus, and also provide the basis for systematically studying its behavioral mechanisms and developing novel control technologies based on behavior regulation.