Peptidoglycan recognition protein AaPGRP-LC is involved in immune responses against bacteria in<em> Aedes aegypti</em> (Diptera: Culicidae)
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (1): 25-35    DOI: 10.16380/j.kcxb.2018.01.004
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Peptidoglycan recognition protein AaPGRP-LC is involved in immune responses against bacteria in Aedes aegypti (Diptera: Culicidae)
YANG Qing-Tai1, ZHAO Li-Lin1,2, ZOU Zhen2, WANG Yan-Hong2,*, MA Rui-Yan1,*
(1. College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)
 Download: PDF (2965 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 The objective of this study is to elucidate the roles of peptidoglycan recognition protein LC (PGRP-LC) in response to bacterial infection in Aedes aegypti. 【Methods】 The mRNA abundance of genes of antimicrobial peptides (AMPs) in mosquitoes at different time after infection of Enterobacter cloacae was measured by quantitative real-time PCR (qPCR). After interference of PGRP-LC by RNA interference (RNAi), the expression profiles of immune-related genes were detected by qPCR. The recombinant protein AaPGRP-LC was expressed by prokaryotic expression system and purified through a Ni2+-NTA agarose column. The quality of the purified recombinant protein was tested by Western blotting. 【Results】 Gram-negative E. cloacae bacteria could activate the expression of genes of AMPs in Ae. aegypti at 6 h post infection. After depletion of AaPGRP-LC, the transcription levels of genes of AMPs were significantly decreased during bacterial infection. The expression levels of immune-related genes involved in Toll and IMD pathways were down-regulated after interference of AaPGRP-LC. Western blotting of the purified recombinant protein AaPGRP-LC showed a clear and unique target band. 【Conclusion】 AaPGRP-LC modulates the transcriptional expression of genes of many essential AMPs and plays an important regulatory role in response to bacterial infection. It is involved in the regulation of IMD pathway and may also regulate the Toll pathway. The obtained recombinant AaPGRP-LC can be used in the follow-up study.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
YANG Qing-Tai
ZOU Zhen
WANG Yan-Hong
MA Rui-Yan
Key wordsAedes aegypti; innate immunity   bacteria   peptidoglycan recognition proteins   antimicrobial peptides     
Cite this article:   
YANG Qing-Tai,ZHAO Li-Lin,ZOU Zhen et al. Peptidoglycan recognition protein AaPGRP-LC is involved in immune responses against bacteria in Aedes aegypti (Diptera: Culicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(1): 25-35.
URL:      or
No references of article
[1] YANG Xiao-Qing, WANG Zheng-Liang, WANG Tian-Zhao, YU Xiao-Ping. Analysis of the bacterial community structure and diversity in the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae) by 16S rRNA high-throughput sequencing[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(2): 200-208.
[2] ZHONG Hai-Ying, ZHANG Ya-Lin, WEI Cong. Comparative morphology and ultrastructure of Malpighian tubules of the mute cicada Karenia caelatata and the coleopterous spittlebug Lepyronia coleoptrata
(Hemiptera: Cicadomorpha) (In English)
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 825-834.
[3] MIAO Yu-Tong, DENG Yao, LIU Yu-Di, HOU Mao-Lin. Identification of bacterial proteins in saliva of adults of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 772-781.
[4] TIAN Xiao-Yan, SONG Fu-Ping, ZHANG Jie, LIU Rong-Mei, ZHANG Xing-Peng, DUAN Jiang-Yan, SHU Chang-Long. Diversity of gut bacteria in larval Protaetia brevitarsis (Coleoptera: Scarabaedia) fed on corn stalk[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 632-641.
[5] TANG Fen-Fen, ZHENG Ya-Qiang, BAI Xing-Rong, CHEN Bin. Changes in the resistance to nucleopolyhedrovirus and the expression levels of the related antimicrobial peptide and heat shock protein genes in Bombyx mori exposed to short-time heat shock[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 505-512.
[6] ZHANG Yong-Dong, Li Jun-Lan, Chen Jin-Hong, Ma-Li, Chen Kang-Kang, Lü Zhi-Qiang. Identification and characterization of peroxiredoxin 2 involved in the antioxidative response induced by bacterial infection in the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae) (In English[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 254-263.
[7] YANG Sen, Li-Yan, Mai Yan-Na, Ruan Run-Tian, Song An-Dong, Wang Feng-Qin, Chen Hong-Ge. Isolation and identification of egg-associated bacteria of Hermetia illucens (Diptera:Stratiomyidae) and their effects on its adult oviposition behavior[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 163-172.
[8] CHEN Jin-Hong, XU Lu, MA Li, 吕Zhi-Qiang . Role of catalase in the antioxidative response induced by Micrococcus luteus and Escherichia coli infection in the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1178-1186.
[9] LIU Xiao-Gai, YANG Ya-Jun, LIAO Qiu-Ju, XU Hong-Xing, LIU Ying-Hong, LU Zhong-Xian. Analysis of the bacterial community structure and diversity in the intestine of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 965-976.
[10] XU Yang, NAN Xiao-Ning , WEI Cong, HE Hong. Seasonal characteristics of gut bacterial communities associated with carpenter ant Camponotus japonicus (Hymenoptera: Formicidae)(In English[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 632-640.
[11] ZHENG Zhi-Hua, XU Xiao-Xia, YU Jing, GAO Yan-Fu, ZHANG Yu-Qing, OUYANG Li-Na , JIN Feng-Liang. A peptidoglycan recognition protein (PxPGRP-SA) regulating the expression of antimicrobial peptides in Plutella xylostella (Lepidoptera: Plutellidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 489-499.
[12] LI Wen-Hon, JIN Dao-Chao, LI Feng-Liang, CHENG Ying. Phenotypic fingerprints of bacterium Erwinia persicina from larval gut of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(4): 456-463.
[13] LI Zhen, HAN Lan-Zhi, LIU Yu-Di, HOU Mao-Lin. Change in the diversity of bacterial community in larval midguts of the striped stem borer, Chilo suppressalis (Lepidoptera: Crambidae), after treatment with Bacillus thuringiensis insecticidal proteins[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 292-300.
[14] LU Yong-Peng, GU Ji-Hai, GAO Yi-Fu, TANG Ting, LIU Feng-Song, HUANG Da-Wei . Functional analysis of peptidoglycan recognition protein PGRP-SC in intestinal immunity of Musca domestica[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 269-277.
[15] ZHANG Mou, YANG Pu, ZHU Jia-Ying, YUAN Yuan, GUI Fu-Rong, GAO Xi, WU Guo-Xing. Analysis of the bacterial diversity in the intestine of larval Procecidochares utilis (Diptera: Trypetidae) based on 16S rDNA gene sequence[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 200-208.
Supported by:Beijing Magtech