Abstract 【Aim】 To sequence and analyze the complete mitochondrial genome of Armigeres subalbatus, and to explore the phylogenetic position of Armigeres in Culicinae based on mitochondrial genome sequences. 【Methods】 The nucleotide sequence of the mitochondrial genome of Ar. subalbatus was determined by using PCR amplification and DNA sequencing. The general features and base composition of Ar. subalbatus mitochondrial genome were analyzed, and the phylogenetic relationship of Ar. subalbatus with seven other species of Culicinae was constructed by using maximum likelihood method (ML) and Bayesian inference (BI) based on the nucleotide sequences of concatenated protein-coding genes (PCGs). 【Results】 The complete mitogenome of Ar. subalbatus is 14 891 bp in length (GenBank accession no.: KY978578), and contains 37 genes, including 13 PCGs, 22 tRNAs and 2 rRNAs. Its gene organization is consistent with those of the known mitogenomes of Culicidae. The mitogenome has a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. All 13 PCGs use the ATN as the initiation codon except COI with TCG, and TAA as the termination codon except COII with the incomplete T. All tRNAs could form the typical cloverleaf structure except tRNASer (AGN), whose DHU-arm is lacking. The phylogenetic relationship of eight species within Culicinae based on the mitochondrial genomes presents as Culex+(Armigeres+Aedes). 【Conclusion】 The phylogenetic relationship of Culicinae constructed based on the mitochondrial genomes shows that Armigeres is closer related to Aedes than to Culex, which is consistent with the result of the traditional morphologybased taxonomy.