Cloning and expression analysis of olfactory receptors gene <em>AcOr</em>10 in the Chinese honeybee, <em>Apis cerana cerana</em> (Hymenoptera: Apidae)
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (3): 292-299    DOI: 10.16380/j.kcxb.2018.03.004
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Cloning and expression analysis of olfactory receptors gene AcOr10 in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)
YANG Le, JIANG Wu-Jun, HE Xu-Jiang, ZENG Zhi-Jiang*
(Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China)
 Download: PDF (3643 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 Olfactory receptor Or10 is one of the important receptor proteins for the perception of pheromone in the honeybee, Apis cerana cerana. The aim of this study is to clone the sequence of Or10 gene, and to analyze the expression levels of Or10 in drone antennae and brains across various physiological status, so as to provide the theoretical basis for further studies on the biological function of this gene. 【Methods】 The drone antennae of A. cerana cerana were collected, from which the total RNA was extracted to clone the sequence of Or10. The relative expression levels of Or10 in antennae and brains of drones, which were sexually immature at hive entrance, sexually mature at hive entrance, sexually immature at frames in hive and sexually mature at frames in hive, were detected using quantitative real-time PCR (qPCR). 【Results】 The cDNA sequence of Or10 gene (named as AcOr10) (GenBank accession no.: MF693365) of A. cerana cerana was obtained, with the length of 914 bp and a coding region of 738 bp, encoding 246 amino acids. The encoded protein has the molecular weight of 28.163 kD and isoelectric point of 5.50. The phylogenetic tree showed that AcOr10 clustered with Or10 of A. mellifera and Or4-like of A. florea. The relative expression levels of AcOr10 in antennae of sexually immature drones at hive entrance and sexually mature ones at frames in hive were significantly higher than those of sexually immature drones at hive entrance and sexually immature drones at frames in hive (P<0.05). However there was no significant difference in the relative expression level of AcOr10 in antennae between sexually mature drones at hive entrance and mature ones at frames in hive (P>0.05). There was also no significant difference in the relative expression level of AcOr10 in antennae between sexually immature drones at hive entrance and immature ones at frames in hive (P>0.05). The relative expression level of AcOr10 in brains of sexually mature drones at hive entrance was significantly higher than those of drones sexually immature at hive entrance, sexually immature at frames in hive and sexually mature at frames in hive (P<0.05), while there was no significant difference among the latter three groups (P>0.05). The relative expression levels of AcOr10 in antennae of drones sexually immature at hive entrance, sexually immature at frames in hive and sexually mature at frames in hive were all significantly higher than those in brains of drones with the same physiological status (P<0.05), but there was no significant difference in the expression level of AcOr10 between antennae and brains of drones sexually mature at hive entrance (P>0.05). 【Conclusion】 It is inferred that AcOr10 may be related with sexual maturity of A. cerana cerana drones, and flight activities have little influence on the expression of AcOr10 in antennae but cause the changes of brains, thereby affecting the mating behaviour of drones.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
YANG Le
JIANG Wu-Jun
HE Xu-Jiang
ZENG Zhi-Jiang
Key wordsApis cerana cerana   drones   olfactory receptor gene   AcOr10   sexual maturity   gene expression     
Cite this article:   
YANG Le,JIANG Wu-Jun,HE Xu-Jiang et al. Cloning and expression analysis of olfactory receptors gene AcOr10 in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(3): 292-299.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.03.004      or     http://www.insect.org.cn/EN/Y2018/V61/I3/292
 
No references of article
[1] XI Xue-Peng, WEI Wei, ZHANG Wei-Xing, WANG Hong-Fang, XU Bao-Hua. Molecular cloning and characterization of transcription factor AP-1 gene in Apis cerana cerana (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(4): 423-431.
[2] YUAN Xiao-Long, Muhammad NAEEM, ZHANG Hong, LIANG Cheng, HUANG Jia-Xing, AN Jian-Dong. Evaluation of reproductive disturbance to Chinese bumblebees by the European bumblebee, Bombus terrestris (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(3): 348-359.
[3] HU Jing-Hua, ZHANG Li-Zhen, LIAO Chun-Hua, JIANG Wu-Jun, ZENG Zhi-Jiang, YAN Wei-Yu. Effects of mixtures of brood pheromone esters on the reared queen quality of the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(3): 300-307.
[4] MA Yue-Yue, LIU Wei, WANG Yong, WANG De-Yi, LEI Piao, RU Yu-Tao, JIANG Yi-Ren, YANG Rui-Sheng, QIN Li. Expression profiling and functional analysis of RR-1 cuticular protein genes ApCP12 and ApCP23 in Antheraea pernyi (Lepidoptera: Saturniidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(2): 188-199.
[5] LIAO Chun-Hua, ZHANG Bo, SHI Jing-Liang, WU Xiao-Bo. Effects of fluvalinate on the quality of reared queens of the western honey bee, Apis mellifera (Hymenoptera: Apidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(2): 218-223.
[6] ZHU Wen-Ya, WU Hai-Hua, ZHANG Xue-Yao, LIU Jiao, ZHANG Jian-Zhen, MA En-Bo. Expression profiles of the cytochrome P450 gene LmCYP6FD3 in Locusta migratoria (Orthoptera: Acrididae) and its role in insecticide detoxification[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 742-749.
[7] LIAO Wen-Li, Bu Xiao-Ling, Jiang Wan-Yi, Liu Ji-Sheng. Influence of lipopolysaccharide on the expression of the Toll-like receptor gene BmToll9-2 in larval Bombyx mori [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 372-378.
[8] WANG Yan-Li, Yang Mei-Ling, Ma En-Bo, Zhang Jian-Zhen. Identification and analysis of the miRNAs targeting key genes involved in cuticle metabolism in Locusta migratoria (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 309-317.
[9] CAI Qing-Ling, Lu Xue-Ying, Ma-Ji. Molecular cloning and expression profiling of a cold stress-related chitinase gene Mpcht19 in the desert beetle Microdera punctipennis (Coleoptera: Tenebrionidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 286-296.
[10] ZHANG Yuan-Chen, Liu Xiang-Dong. Cloning and expression analysis of lysozyme gene AgLYS in the cotton-melon aphid,Aphis gossypii (Hemiptera: Aphididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 127-135.
[11] WANG Yong, PENG Chu-Yue, WU Jin-Xin, SHEN Guan-Wang, LIN Ying, XIA Qing-You. Regulation of the expression of vitellogenin gene by vertebrate estrogen and a microscale thermophoresis assay of its interaction with ecdysone receptors in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(12): 1367-1375.
[12] JI Xiang-Long, ZHAN Yi-Di, LI Pei-Ling, LIU Yong. Spatio-temporal expression patterns of (E)-β-farnesene-binding protein genes in the turnip aphid, Lipaphis erysimi (Hemiptera: Aphididae) (in English)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(12): 1376-1383.
[13] ZHAN 恩Ling, DU Xiao, ZHAO Ai-Ping, SUN Cong, LIU Tong-Xian, LI Yi-Ping. Gene cloning and expression of polycalin protein from Plutella xylostella (Lepidoptera: Plutellidae) and its binding characteristics with Cry1Ac toxin[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1155-1167.
[14] HAO You-Jin, XU Yan-Ling, CHEN Bin. Mining the secretome of Delia antiqua (Diptera: Anthomyiidae) based on the transcriptomic and gene expression data and analysis of its potential roles in diapause development (In English[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1168-1177.
[15] LI Guang-Wei, CHEN Xiu-Lin, SHANG Tian-Cui. cDNA cloning, expression and ligand binding properties of the odorant binding protein AglaOBP12 in the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1141-1154.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech