Comparative transcriptome analysis of salivary glands of <em>Southern rice black-streaked dwarf virus</em> (SRBSDV)infected and uninfected adults of the whitebacked planthopper, <em>Sogatella furcifera</em> (Hemiptera: Delphacidae)
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ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (4): 449-457    DOI: 10.16380/j.kcxb.2018.04.007
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Comparative transcriptome analysis of salivary glands of Southern rice black-streaked dwarf virus (SRBSDV)infected and uninfected adults of the whitebacked planthopper, Sogatella furcifera (Hemiptera: Delphacidae)
DENG Yao1,2, LIU Yu-Di1,2,*, WANG Xiang-Ping1,*, HOU Mao-Lin2
  (1. Institute of Insect, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025; China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
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Abstract 【Aim】 The white-backed planthopper (WBPH), Sogatella furcifera, is a main vector of Southern rice black-streaked dwarf virus (SRBSDV). The salivary glands of the WBPH play an important role in feeding behaviour and virus transmission. This study aims to sequence the salivary gland transcriptomes of viruliferous (SRBSDV-infected) and nonviruliferous adults of the WBPH, to find the differentially expressed genes in salivary glands and to further infer the virus-related genes. 【Methods】 Salivary gland transcriptomes of viruliferous and nonviruliferous adults of S. furcifera were sequenced by using the Ion Proton II, PGM platform, and then were de novo assembled by SOAPdenovo software. Gene annotation was conducted by blastX, and GO term enrichment and KEGG metabolic pathway analysis were performed using Blast2go and Blastall software. The differentially expressed genes were calculated by RPKM values. 【Results】 A total of 52 062 unigenes from viruliferous salivary glands (VSGs) and 51 407 unigenes from nonviruliferous salivary glands (NVSGs) of S. furcifera with the mean length of 639 and 647 bp, respectively, were obtained, and their GenBank accession numbers are SRS851833 and SRS843978, respectively. A total of 18 431 unigenes have homologous sequences against the NR database, and the highest percentage of unigene sequences (16.23%) were matched to genes of Tribolium castaneum. All unigenes were enriched to 52 GO terms and 240 KEGG pathways. The results revealed that 89 unigenes had a significantly different expression level between VSGs and NVSGs. 【Conclusion】 Through the comparative analysis, the differentially expressed genes between viruliferous and nonviruliferous salivary glands of S. furcifera were found, and some of these genes might be involved in virus-vector interactions. The gene expression characteristics of salivary gland transcriptome provide useful information for the identification of genes involved in feeding and virus transmission and so lay the basis for investigating the interaction between SRBSDV and WBPH at the molecular level.
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DENG Yao
LIU Yu-Di
WANG Xiang-Ping
Hou-Mao-Lin
Key wordsSogatella furcifera; salivary glands   Southern rice black-streaked dwarf virus (SRBSDV)   transcriptome   differentially expressed genes     
Cite this article:   
DENG Yao,LIU Yu-Di,WANG Xiang-Ping et al. Comparative transcriptome analysis of salivary glands of Southern rice black-streaked dwarf virus (SRBSDV)infected and uninfected adults of the whitebacked planthopper, Sogatella furcifera (Hemiptera: Delphacidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(4): 449-457.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.04.007      or     http://www.insect.org.cn/EN/Y2018/V61/I4/449
 
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