Analysis of genetic differentiation of different geographical populations of the melon fly, Bactrocera cucuribitae (Diptera: Tephritidae) in China based on microsatellite molecular markers
ZHANG Ya-Nan1,2, NIU Li-Ming1, ZHOU Shi-Hao2, GONG Zhi1, LI Lei1, ZHANG Fang-Ping1, HAN Dong-Yin1, FU Yue-Guan1,*
(1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China)
Abstract 【Aim】 The melon fly, Bactrocera cucuribitae is an important invasive pest with wide host range, causing serious harm. This study aims to explore its population differentiation and genetic variation in China. 【Methods】 The genetic diversity of a total of 190 individuals collected from 21 regions of 7 provinces in China was analyzed using nine polymorphic microsatellite loci as the molecular markers. 【Results】 For the 21 geographical populations of B. cucuribitae, the average percentage of polymorphic loci was 97.08%, Shannon’s diversity index(I) was 0.8841, and the genetic differentiation coefficient among populations (FST) was 0.12806, suggesting that genetic differentiation occurs among the 21 geographical populations. The UPGMA cluster analysis showed that the populations of Hainan (excluding Sansha), Guangdong, Guangxi, Yunnan and Sansha of Hainan were clustered into one clade, while the populations of Fujian, Jiangxi and Sichuan clustered into separate clades, respectively. 【Conclusion】 A certain degree of genetic differentiation has occurred among the melon fly populations in China, but the accumulated variability is limited.
ZHANG Ya-Nan,NIU Li-Ming,ZHOU Shi-Hao et al. Analysis of genetic differentiation of different geographical populations of the melon fly, Bactrocera cucuribitae (Diptera: Tephritidae) in China based on microsatellite molecular markers[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(5): 613-619.