Molecular cloning, tissue expression profiling and binding characterization of the pheromone binding protein SlitPBP4 from <em>Spodoptera litu</em>ra (Lepidoptera: Noctuidae)
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (6): 657-667    DOI: 10.16380/j.kcxb.2018.06.004
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Molecular cloning, tissue expression profiling and binding characterization of the pheromone binding protein SlitPBP4 from Spodoptera litura (Lepidoptera: Noctuidae)
SUN Jia-Bin1, LIU Nai-Yong2, LI Shuang-Mei1, YAN Qi1, DONG Shuang-Lin1,* 
 (1. Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China)
 Download: PDF (4017 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract  【Aim】 This study aims to identify the new pheromone binding protein (PBP) gene of Spodoptera litura, to clarify its tissue expression pattern and to explore its functions. 【Methods】 Based on the sequence characteristics of PBP4 homologues and their vicinity with other PBP genes on the chromosome in four non-noctuid species (Manduca sexta, Bombyx mori, Danausplexippus and Melitaea cinxia) reported previously, a PBP4 gene was obtained from S. litura by analyzing S. litura PBP/GOBP genes previously cloned by our laboratory. The tissue expression patterns of this gene in male and female adults were determined by RT-PCR and qPCR. The binding properties of the recombinant PBP4 protein to sex pheromone components and plant odorants were measured by fluorescence competitive binding assay. 【Results】 The first PBP4 gene in Noctuidae was identified from S. litura and named SlitPBP4, which was deposited in GenBank under accession number MG356847. The cDNA sequence of SlitPBP4 encodes 210 amino acids, with the typical sequence features of PBPs, including an N terminal signal peptide, several hydrophobic regions, six conserved cysteines, and two introns inserted at the conserved positions in the genomic DNA. However, SlitPBP4 has a C terminus much longer than that of the other three reported SlitPBPs. SlitPBP4 showed predominantly high expression level in the male abdomen (the reproductive systems) but nearly undetectable in the antennae and other adult tissues. Fluorescence competitive binding assay showed that SlitPBP4 protein had no obvious binding ability to the tested pheromone components and plant odors. 【Conclusion】 The first PBP4 gene of noctuids was reported. SlitPBP4 is likely to be involved primarily in the reproduction-related physiological processes in male adults, rather than the olfactory function of other reported PBP genes in noctuids.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
SUN Jia-Bin
LIU Nai-Yong
LI Shuang-Mei
DONG Shuang-Lin
Key wordsSpodoptera litura   SlitPBP4   tissue expression   prokaryotic expression   fluorescence competitive binding assay     
Cite this article:   
SUN Jia-Bin,LIU Nai-Yong,LI Shuang-Mei et al. Molecular cloning, tissue expression profiling and binding characterization of the pheromone binding protein SlitPBP4 from Spodoptera litura (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 657-667.
URL:      or
No references of article
[1] YUE Wen-Bo, ZHI Jun-Rui, LIU Li, YE Mao, ZHANG Xiang-Qian, ZENG Guang- . Effects of pest insect feeding and mechanical damage on the defensive enzyme activities in leaves at different parts of kidney bean plants[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 860-870.
[2] CHEN Xiu-Lin, SU Li, CHEN Li-Hui, LI Yi-Ping, WU Jun-Xiang, LI Guang-Wei. Molecular cloning, expression profiling and binding characterization of a Minus-C odorant binding protein from the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 771-783.
[3] SI Pin-Fa, ZHOU Qiong, CUI Zhong-Yi. Cloning, prokaryotic expression and tissue expression profiling of an odorant binding protein gene BminOBP25 from Bactrocera minax (Diptera: Tephritidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(5): 537-545.
[4] CHENG Xing-An, LIN Xian-Wei, JIANG Xu-Hong, LIU Zhan-Mei, ZHONG Guo-Hua, Hu-Mei-Ying. cDNA cloning, prokaryotic expression and polyclonal antibody preparation of cathepsin L in Spodoptera frugiperda (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(4): 410-422.
[5] LIU Xiao-Long, ZHANG Bin, TIAN Hao-Kai, NING Jing, WANG Hai-Xiang, ZHAO Li-Lin. Cloning, prokaryotic expression and expression profiling of Mfe2 from the Japanese sawyer beetle, Monochamus alternatus (Coleoptera: Cerambycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(3): 282-291.
[6] SHI Zong-Pan, RAN Yong-Hong, ZHANG Jing-Jing, ZHANG Jing, YAN Zhen-Tian, CHEN Bin, HE Zheng-Bo. Binding characteristics of the odorant binding protein AsinOBP1 of Anopheles sinensis (Diptera: Culicidae) with the mosiquito repellent DEET[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(1): 139-148.
[7] MIAO Ya, ZHANG Tian-Tian, XU Bai-Ying, CHEN Bin. Expression, purification and crystallization of the glutathione-S-transferase E6(AsGSTE6) from Anopheles sinensis (Diptera: Culicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(1): 59-67.
[8] TAN Yong-An, ZHAO Xu-Dong, XIAO Liu-Bin, SUN Yang, ZHAO Jing, BAI Li-Xin, HAO De-Jun. Cloning and characterization of the nuclear receptor gene AlE75D in Apolygus lucorum (Hemiptera: Miridae)  [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(9): 984-993.
[9] ZHAO Yu, LIU Zhong-Yuan. Cloning, expression and functional verification of the serine protease inhibitor gene ApSerpin-FA72 from Anatolica polita borealis (Coleoptera: Tenebrionidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(8): 857-864.
[10] LI Zhao-Qun, ZHANG Shuai, ZHOU Shu-Fen, LUO Jun-Yu, CUI Jin-Jie. Tissue expression profiling and ligandbinding properties of HarmOBP16 of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(8): 891-899.
[11] YANG Ye-Qing, WANG Shan-Ning, PENG Yong, DAN Shuang, ZHENG Yao, LI Rui-Jun, ZHANG Yong-Jun, GUO Yu-Yuan. Expression of the odor binding protein MmedOBP19 in the legs of Microplitis mediator (Hymenoptera: Braconidae) and its ligand binding characteristics[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(6): 613-620.
[12] YANG Ji-Gui, PENG Li-Na, LIU Tai-Xing, WU Yun-Fei, DU Guo-Yu, ZHANG Qian, WANG Wei, PAN Min-Hui. Cloning and expression analysis of the Sox family gene BmDichaete in the silkworm, Bombyx mori[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 513-522.
[13] ZHANG Yuan-Chen, Liu Xiang-Dong. Cloning and expression analysis of lysozyme gene AgLYS in the cotton-melon aphid,Aphis gossypii (Hemiptera: Aphididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 127-135.
[14] TAN Yong-An, ZHAO Xu-Dong, HAO De-Jun, XIAO Liu-Bin, BAI Li-Xin, ZHAO Jing, SUN Yang, JIANG Yi-Ping. Expression, purification and enzymatic characteristics of phosphodiesterase C (AlPLC) of Apolygus lucorum (Hemiptera: Miridae)
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(11): 1247-1254.
[15] LI Guang-Wei, CHEN Xiu-Lin, SHANG Tian-Cui. cDNA cloning, expression and ligand binding properties of the odorant binding protein AglaOBP12 in the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1141-1154.
Supported by:Beijing Magtech