Weissella paramesenteroides facilitates the systemic growth of <em>Drosophila melanogaster</em> by modulating ecdysone and insulin signaling pathways (<em>In English</em>)
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ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (6): 676-685    DOI: 10.16380/j.kcxb.2018.06.006
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Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)
LI En-Hui1, WANG Xiao-Yang3, ZHANG Yue-Xiao3, BAI Peng3, ZHAO Xin1, LIU Wei2,*, ZHANG Ce1,*
(1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China; 3. Department of Basic Medical, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China)
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Abstract 【Aim】 To investigate the effects of lactic acid bacteria Weisellas paramesenteroides on the growth and development of Drosophila melanogaster. 【Methods】 Bacterium was isolated from the gut of D.melanogaster adults with selective medium and identified by BLAST analysis of 16S rRNA gene. The developmeantal duration of D. melanogaster was quantified by counting the time to puparium formation and adult eclosion, and the growth rate was calculated as the body surface area of larvae. The expression levels of genes involved in hormoral (dib, E74B and PTTH) and insulin signaling(DILP2, DILP3 and InR)of D. melanogaster at different time after egg deposition were assayed by quantitative real-time PCR. The glucose level in the hemolymph of the 3rd instar larvae was measured by glucose oxidase assay. 【Results】 W. paramesenteroides was isoloated and efficiently colonized in the gut of D. melanogaster adults. W. paramesenteroides shortened the time to puparium formation and adult eclosion of D. melanogaster by enhancing the growth rate of flies. W. paramesenteroides increased the expression levels of dib, E74B and PTTH, as well as the expression of DILP2 and DILP3, while decreased the expression levels of InR and the glucose level in larval hemolymph.【Conclusion】 W. paramesenteroides is the symbiotic bacterium of D. melanogaster, and promotes its development by activting the ecdysone and insulin signaling pathways.
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LI 恩Hui
WANG Xiao-Yang
ZHANG Le-Xiao
BAI Peng
ZHAO Xin
LIU Wei
ZHANG Ce
Key wordsWeissella paramesenteroides   Drosophila melanogaster   development   ecdysone signaling pathway   insulin signaling pathway     
Cite this article:   
LI 恩Hui,WANG Xiao-Yang,ZHANG Le-Xiao et al. Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 676-685.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.06.006      or     http://www.insect.org.cn/EN/Y2018/V61/I6/676
 
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