Composition and diversity of microbes in the infrabuccal pocket of <em>Camponotus japonicus</em> (Hymenoptera: Formicidae)
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (6): 686-697    DOI: 10.16380/j.kcxb.2018.06.007
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Composition and diversity of microbes in the infrabuccal pocket of Camponotus japonicus (Hymenoptera: Formicidae)
ZHANG Kai-Xuan1, WEI Cong2, NAN Xiao-Ning1, WANG Yun-Guo1, HE Hong1, *
 (1. Key Laboratory of Comprehensive Forestry of Shaanxi Province, College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China)
 Download: PDF (3909 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to investigate the microbe composition (actinomycetes, bacteria and fungi) and the diversity of bacteria in the infrabuccal pocket of the Japanese carpenter ant, Camponotus japonicus, so as to explore their potential functions in food utilization and population immunity. 【Methods】 The culturedependent method and HiSeq high-througput sequencing technique were combined to analyze the microbes in the infrabuccal pocket, digestive tract and cuticle of C. japonicus workers collected in Yangling, Shaanxi, northwestern China. 【Results】 Ten actinobacterial strains were isolated from the infrabuccal pocket of C. japonicus workers, among which five strains belong to Streptomyce with the average isolation frequencies of 73.3%-96.7%. Seven bacterial strains were isolated and four of them were Bacillus, and the average isolation frequencies of the strains N-B1 and N-B4 were both above 70%. Three fungal strains were isolated, and the isolation frequency of the dominant strain P-F1 (Wickerhamiella) was up to 96.7%. All strains isolated from the crop, midgut and cuticle could be found in the infrabuccal pocket, and the species number of the strains and the number of microbial colonies isolated from the infrabuccal pocket were higher than those isolated from the cuticle, crop and midgut. The results of HiSeq high-throughput sequencing showed that the dominant bacterial groups in the infrabuccal pocket mainly belong to Proteobacteria, Firmicutes and Actinobacteria, but in the crop and midgut the dominant bacterial groups belong to Proteobacteria and Firmicutes. The dominant genera with high abundance were more in the infrabuccal pockets and crops than in the midgut, and the bacterial composition in the midgut was relatively simple. The dominant genera in infrabuccal pocket included Fructobacillus, Bacterium, Pseudomonas, Acinetobacter and Sphingtomonas. The microbial abundance and diversity in the infrabuccal pocket were higher than those in the crop and midgut. 【Conclusion】 Actinomycetes, Bacillus, yeasts and other predominant microbes generally exist in the infrabuccal pocket of C. japonicus, and their abundance and diversity levels are significantly higher than those in the digestive tract (crop and midgut). The potential roles of these microorganisms in food utilization, nutrient digestion and population immunity of ants need to be further studied.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZHANG Kai-Xuan
WEI Cong
南Xiao-Ning
WANG Yun-Guo
HE Hong
Key wordsCamponotus japonicus   infrabuccal pocket   digestive tract   microbiological cultivation   HiSeq high-throughput sequencing     
Cite this article:   
ZHANG Kai-Xuan,WEI Cong,南Xiao-Ning et al. Composition and diversity of microbes in the infrabuccal pocket of Camponotus japonicus (Hymenoptera: Formicidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 686-697.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.06.007      or     http://www.insect.org.cn/EN/Y2018/V61/I6/686
 
No references of article
[1] XU Yang, NAN Xiao-Ning , WEI Cong, HE Hong. Seasonal characteristics of gut bacterial communities associated with carpenter ant Camponotus japonicus (Hymenoptera: Formicidae)(In English[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 632-640.
[2] CHEN Hong-Hong,PAN H Z, ZHANG S Z. Identification and localization of endocrine cells in the digestive tract of Oxya chinensis (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2009, 52(7): 749-754.
[3] LIU Shu-Yu, HUA B Z. Morphology and histology of the alimentary canal in scorpionfly Panorpa obtusa (Mecoptera: Panorpidae)[J]. ACTA ENTOMOLOGICA SINICA, 2009, 52(7): 808-813.
[4] ZHANG Xiao-Min, GUO Ya-Ping, XU Jing, MA En-Bo. Microstructure of the inner alimentary canal in Oxya chinensis (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2006, 49(2): 219-229.
[5] Bassey E. AKPAN1, Tony G.OKORIE2. Microstructural adaptation of Zonocerus variegates (L.) (Orthoptera: Pyrgomorphidae) gut for food storage, digestion and absorption[J]. ACTA ENTOMOLOGICA SINICA, 2005, 48(3): 325-330.
[6] TAN Sheng-jiang1, CHEN Xiao-feng1, WANG Zheng-jun1, LIU Zhi-bin2. Studies on kin recognition of Camponotus japanicus: test of aggressive behavior and RAPDPCR analysis[J]. ACTA ENTOMOLOGICA SINICA, 2001, 44(3): 373-377.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech