Screening of microsatellite loci and the genetic diversity analysis of <em>Mythimna separata</em> (Lepidoptera: Noctuidae) populations in China
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (6): 712-720    DOI: 10.16380/j.kcxb.2018.06.010
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Screening of microsatellite loci and the genetic diversity analysis of Mythimna separata (Lepidoptera: Noctuidae) populations in China
LI Mei-Mei#, LI Bo-Liao#, WU Jun-Xiang*, XU Xiang-Li* 
(State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China)
 Download: PDF (1032 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to acquire microsatellite loci available for studying the population genetics of the oriental armyworm, Mythimna separata in China and to further uncover the genetic diversity of its geographical populations from a molecular perspective. 【Methods】 Based on microsatellite markers which had been previously reported in public and  the simple sequence repeats (SSRs) from our laboratory transcriptome of M. separata, the stability of amplification and polymorphism of these microsatellite loci were analyzed based on 200 individuals sampled in seven geographical populations from Henan, Shaanxi and Shanxi in China. The PCR products of SSRs were labeled fluorescently and scanned automatically. 【Results】 Seven microsatellite loci were amplified stably in samples of seven geographical populations of M. separata and possessed high polymorphism. The allele richness (Ar) of the seven microsatellite loci was between 4.167 and 12.402, the average observed heterozygosity (Ho) and expected heterozygosity (He) were 0.640 and 0.752, respectively, and the polymorphic information content (PIC) ranged from 0.547 to 0.884. The seven loci had null allele and deviated from Hardy.Weinberg equilibrium. No significant linkage disequilibrium existed in pairwise loci. 【Conclusion】 Seven microsatellite loci were successfully screened from seven geographical populations of M. separata from Henan, Shaanxi and Shanxi, and they show high polymorphism among these geographical populations and can be used in genetic structure research of this insect. In addition, frequent gene flow occurs in these populations, which prevents population differentiation caused by genetic drift. Moreover, fairly low even no pairwise population differentiation occurs in these populations.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
LI Mei-Mei
LI Bo-Liao
WU Jun-Xiang
XU Xiang-Li
Key wordsMythimna separata   microsatellite marker   amplifying stability   genetic diversity   genetic differentiation   gene flow     
Cite this article:   
LI Mei-Mei,LI Bo-Liao,WU Jun-Xiang et al. Screening of microsatellite loci and the genetic diversity analysis of Mythimna separata (Lepidoptera: Noctuidae) populations in China[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 712-720.
URL:      or
No references of article
[1] GUO Pei, MA Bai-Wei, ZHAO Xin-Cheng, WANG Gao-Ping, XIE Gui-Ying. Distribution of 5-hydroxytryptamine in the optic lobes of the oriental armyworm, Mythimna separata (Lepidotpera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 668-675.
[2] ZHANG Ya-Nan, NIU Li-Ming, ZHOU Shi-Hao, GONG Zhi, LI Lei, ZHANG Fang-Ping, HAN Dong-Yin, FU Yue-Guan. Analysis of genetic differentiation of different geographical populations of the melon fly, Bactrocera cucuribitae (Diptera: Tephritidae) in China based on microsatellite molecular markers[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(5): 613-619.
[3] LI Hong-Bo, DAI Chang-Geng, ZHANG Chang-Rong, HE Yong-Fu, HU Yang. Cloning and expression profiling of catalase gene in the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(2): 178-187.
[4] YANG Shun-Yi, ZHOU Xing-Long, SONG Li-Wen, YAN Xiao-Tong, ZHANG Xiao-Yun, ZHANG Xin-Hu, SHEN Hui-Min. Analysis of genetic differentiation among different geographic populations of Tetranychus truncatus (Acari: Tetranychidae) in Gansu province, northwestern China based on mtDNA COI gene  [J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(9): 1083-1092.
[5] LIU Lin, Zhang-欧, Ma-Shu-Jie, Ma-Zhi-Qing, Zhang-Xing. Effects of wilforgine on flight muscular structure in adult Mythimna separata (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 782-789.
[6] ZHU Shi-Yu, Xu-Wei, Gao-Yu, Cui-Juan, OU Shi-Qi, Shi Shu-Sen. Analysis of the genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera: Olethreutidae) in China based on mitochondrial COII gene sequences[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 475-486.
[7] WANG Juan, LI Bo-Liao, WU Jun-Xiang, XU Xiang-Li. Effects of fluctuating temperature on the reproduction and metabolism of primary energy substances in Mythimna separata (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 917-924.
[8] GUO Hui-Ping, ZHOU Shu-Jing, ZHU Xiang-Jie, XU Xin-Jian, YU Ying-Long, YANG Kai-Jie, CHEN Dao-Yin, ZHOU Bing-Feng. Population genetic analysis of Apis cerana cerana from the Qinling-Daba Mountain Areas based on microsatellite DNA[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 337-345.
[9] GU Ying, BIN Xian-Li, LIU Lu, ZHOU Jun, MA Zhen-Gang, TAN Hong-Wei, ZHOU Ze-Yang, XU Jin-Shan. Morphological and genetic diversity of the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae) in Chongqing, southwestern China[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(12): 1386-1394.
[10] ZHANG Peng-Fei, ZHOU Xiao-Rong, PANG Bao-Ping, CHANG Jing, SHAN Yan-Min, ZHANG Zhuo-Ran. Microsatellite marker analysis of the genetic diversity of Galeruca daurica (Coleoptera: Chrysomelidae) populations from Inner Mongolia[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(9): 1005-1011.
[11] ZHOU Lin, LI Jun-Ling, LIU Xiang-Yang, MA Zhi-Qing, FENG Jun-Tao, ZHANG Xing. Effects of total alkaloids from Tripterygium wilfordii (Celastraceae) on the activities of neural enzymes and contents of neurotransmitters in larval Mythimna separata (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(8): 856-863.
[12] LOU Heng, CHEN Ya-Ting, ZHANG Yan-Kai, YANG Xian-Ming, SUN Jing-Tao, HONG Xiao-Yue. Wolbachia infection and its relationship with mtDNA diversity in the flower thrips, Frankliniella intonsa (Thysanoptera: Thripidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(7): 750-760.
[13] HE Qiang, LIU Shao-Lun, MA Zheng-Gang, ZHANG You-Yi, Charles R. VOSSBRIN. Comparative genomics of three geographical strains reveals genetic diversity in Antheraea pernyi nucleopolyhedrovirus (AnpeNPV)(In English[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(5): 535-545.
[14] HE Yue-Qiu, CHI Shu-You, DU Yong-Jun. Identification of female sex pheromones of Diaphania perspectalis (Lepidoptera: Crambidae) and their attractiveness to moths[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(10): 1081-1090.
[15] Arvind SHARMA, Richa SHARMA, Ashwani KUMAR, Madhulika DUBE, Surendra Kumar GAKHAR. Gene flow and population genetic structure of Anopheles stephensi (Diptera: Culicidae) in the areas under Enhanced Malaria Control Project (EMCP) and non-EMCP of Madhya Pradesh, Central India using microsatellite markers(In English)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(1): 74-81.
Supported by:Beijing Magtech