Change of carbohydrate, protein and lipid contents in <em>Galeruca daurica</em> (Coleoptera: Chrysomelidae) adults during oversummering
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ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (7): 808-814    DOI: 10.16380/j.kcxb.2018.07.007
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Change of carbohydrate, protein and lipid contents in Galeruca daurica (Coleoptera: Chrysomelidae) adults during oversummering
CHEN Long1, ZHOU Xiao-Rong1, GAO Li-Jun2, TAN Yao1, PANG Bao-Ping1,*
 (1. Research Center for Grassland Entomology, Hohhot 010010, China; 2. Grassland Station of Xilinhaote City, Xilinhaote, Inner Mongolia 026000, China)
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Abstract 【Aim】 This study aims to clarify the characteristics of main physiological and biochemical change in Galeruca daurica adults at different oversummering stages so as to lay a foundation for revealing the oversummering mechanism. 【Methods】 The contents of total sugar, trehalose, glycogen, lipid and water in G. daurica adults at different oversummering stages (pre-oversummering, during oversummering and post-oversummering) were determined by anthrone-sulfuric acid and methanol-chloroform methods, respectively, and the total protein content was detected by Coomassie brilliant blue G-250 method. 【Results】 The contents of total sugar, water and total protein in G. daurica adults were significantly higher at the pre-oversummering stage (26.81 μg/mg, 77.47% and 63.17 μg/mg, respectively) and post-oversummering stage (26.41-26.85 μg/mg, 75.29%-76.65% and 59.53-64.93 μg/mg, respectively) than during oversummering (18.77-21.14 μg/mg, 61.50%-67.20% and 39.82-52.54 μg/mg, respectively), whereas the change trend of glycogen contents was the opposite, being 8.43 μg/mg at the pre-oversummering stage, 5.91-6.14 μg/mg at the post-oversummering stage and 10.18-11.58 μg/mg during oversummering, respectively, and the content of lipid was significantly less at the post-summering stage (11.48%-11.65%) than at the pre-oversummering stage (42.48%) and during oversummering (36.05%-64.79%). The contents of lipid and total protein decreased gradually in G. daurica adults during oversummering, while the water, total sugar and glycogen contents showed no obvious change. The trehalose content in G. daurica adults at different oversummering stages fluctuated greatly from 2.66 μg/mg to 25.31 μg/mg. 【Conclusion】 Lipid and glycogen are the main energy materials of G. daurica adults during oversummering.
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CHEN Long
ZHOU Xiao-Rong
GAO Li-Jun
TAN Yao
PANG Bao-Ping
Key wordsGaleruca daurica; oversummering   diapause   carbohydrate   glycogen   trehalose   protein   lipid     
Cite this article:   
CHEN Long,ZHOU Xiao-Rong,GAO Li-Jun et al. Change of carbohydrate, protein and lipid contents in Galeruca daurica (Coleoptera: Chrysomelidae) adults during oversummering[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 808-814.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.07.007      or     http://www.insect.org.cn/EN/Y2018/V61/I7/808
 
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