Bioefficacy of the combined application of entomopathogenic nematodes and thiamethoxam and its effects on the protective and detoxification enzyme activities in Bradysia odoriphaga (Diptera: Sciaridae) larvae
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (7): 851-859    DOI: 10.16380/j.kcxb.2018.07.012
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Bioefficacy of the combined application of entomopathogenic nematodes and thiamethoxam and its effects on the protective and detoxification enzyme activities in Bradysia odoriphaga (Diptera: Sciaridae) larvae
WU Hai-Bin1, GONG Qing-Tao1, CHEN Zhen-Zhen2, JIANG Li-Li1, GONG Yi3, XU Yong-Yu2, SUN Rui-Hong1,* 
(1. Shandong Institute of Pomology, Tai′an, Shandong 271000, China; 2. College of Plant Protection, Shandong Agricultural University, Tai′an, Shandong 271018, China; 3. Shandong Plant Protection Station, Jinan 250100, China)
 Download: PDF (3990 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 Pathogenic organisms or chemical pesticides can induce the defense responses of insects, and the physiological adaptation of insects might differ under the combined application of pathogenic organisms and chemical pesticides. This study aims to investigate the effects of combined application of entomopathogenic nematodes and thiamethoxam on Bradysia odoriphaga. 【Methods】 The LT50 value of the combined application of Steinernema feltiae SF-SN strain (Sf) (60 IJs/larva) and thiamethoxam (15 mg/L) against the 3rd instar larvae of B. odoriphaga was determined with filter paper culture method in the laboratory, and its effects on the protein content of enzyme solution and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferases (GSTs) and acetylcholinesterase (AChE) in B. odoriphaga larvae at different time after treatment were assayed by biochemical analysis. 【Results】 The LT50 value (41.05 h) of the combined application of Sf and thiamethoxam against the 3rd instar larvae of B. odoriphaga decreased by about 126.88 h and 31.77 h, respectively, as compared with those in treatments with the respective single agents with (Sf LT50: 167.93 h) and thiamethoxam (LT50: 72.82 h). During 24-72 h after treatment, the corrected mortality rates of B. odoriphaga larvae in the combined treatment group were significantly higher than those in treatments with the respective single agent, and reached the peak (96.61%) at 72 h. The protein contents of enzyme solution in B. odoriphaga larvae in the combined treatment group at 12, 24 and 36 h after treatment increased by 13.88%, 46.87% and 57.99%, respectively, as compared with the control group, and were significantly higher than those in the control group, Sf treatment group and thiamethoxam treatment group. The activities of SOD, CAT, AChE and GTSs in larvae in the combined treatment group decreased by 47.48%, 28.73%, 71.04% and 29.97% at 24 h after treatment, and by 46.34%, 4222%, 58.37% and 11.87% at 36 h, respectively, as compared with the control. The activities of the four enzymes in the combined treatment group were significantly lower than those in the control group, Sf treatment group and thiamethoxam treatment group at 24 h and 36 h after treatment. 【Conclusion】 The LT50 value of the combined application of Sf and thiamethoxam against the 3rd instar larvae of B. odoriphaga is significantly lower than those in treatments with the respective single agent, and the combined treatment can significantly restrain the activities of SOD, CAT, AChE and GTSs in larvae.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WU Hai-Bin
GONG Qing-Tao
CHEN Zhen-Zhen
JIANG Li-Li
GONG Yi
XU Yong-Yu
SUN Rui-Hong
Key wordsBradysia odoriphaga   entomopathogenic nematode   Steinernema feltiae SF-SN   thiamethoxam   enzyme activity     
Cite this article:   
WU Hai-Bin,GONG Qing-Tao,CHEN Zhen-Zhen et al. Bioefficacy of the combined application of entomopathogenic nematodes and thiamethoxam and its effects on the protective and detoxification enzyme activities in Bradysia odoriphaga (Diptera: Sciaridae) larvae[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 851-859.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.07.012      or     http://www.insect.org.cn/EN/Y2018/V61/I7/851
 
No references of article
[1] YUE Wen-Bo, ZHI Jun-Rui, LIU Li, YE Mao, ZHANG Xiang-Qian, ZENG Guang- . Effects of pest insect feeding and mechanical damage on the defensive enzyme activities in leaves at different parts of kidney bean plants[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 860-870.
[2] QIAN Xue, WANG Yue-Ying, XIE Huan-Huan, DOU Jie, LI Zhan-Wu, Roman JASHENKO, JI Rong. Effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of Gomphocerus sibiricus (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 499-504.
[3] JIANG Li-Na, Qian-Lei, Xi-Chao, Wang Xue-Li, Yang-Lu, Gui Fu-Rong. Effects of elevated CO2 on the digestive enzyme activities in the adults of Frankliniella occidentalis and F. intonsa (Thysanoptera: Thripidae) on different host plants[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 237-246.
[4] TAN Yong-An, ZHAO Xu-Dong, HAO De-Jun, XIAO Liu-Bin, BAI Li-Xin, ZHAO Jing, SUN Yang, JIANG Yi-Ping. Expression, purification and enzymatic characteristics of phosphodiesterase C (AlPLC) of Apolygus lucorum (Hemiptera: Miridae)
 
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(11): 1247-1254.
[5] XU Lei, ZHAO Tong-Hua, LIU Pei-Bin, XU Guo-Qing, WANG Zhe, ZHONG Tao. Optimization of fumigation conditions of dichlorvos smoke agent in controlling Bradysia odoriphaga (Diptera: Sciaridae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1198-1207.
[6] ZHI Jun-Rui, TIAN Tian, WEN Juan, LIU Yong. Effects of kidney bean damaged by Frankliniella occidentalis (Thysanoptera: Thripidae) or Tetranychus urticae (Acari: Tetranychidae) on the activities of protective and detoxification enzymes in the other subsequent herbivore of both[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(7): 707-715.
[7] LI Na, FAN Fan, HAN Hui, PEI Xiao-Ya, LIU Shun, WEI Guo-Shu. Effects of LED lights at different wavelengths on the reproductive behaviors of Bradysia odoriphaga (Diptera: Sciaridae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(5): 546-551.
[8] FU Ying-Ying, MA Yu-Kun, REN Shu-Wen, YANG Li-Jun, LIU Chun-Guang, FU Hai-Yan, HOU Yue-Ying, YANG Feng-Shan. Changes in physiological indices of Antheraea pernyi (Lepidoptera: Saturniidae) pupae induced by gram-positive bacteria  Bacillus thuringeinsis and gramnegative bacteria Escherichia coli[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 192-199.
[9] SHI Cai-Hua, HU Jing-Rong, XU Yue-Qiang, YANG Yu-Ting, CHENG Jia-Xu, ZHANG You-Jun. Control efficacy of ozone water against the chive gnat, Bradysia odoriphaga (Diptera: Sciaridae) and its influences on the growth and seed germination of Chinese chives[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(12): 1354-1362.
[10] YANG Ni-Na, ZHANG You-Jun, YANG Xin, HUANG Da-Ye, LONG Tong, WAN Peng. Differential expression of the detoxification enzyme genes in different developmental stages of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(11): 1166-1173.
[11] HAO Ai-Ping, SUN Cong, ZHAN En-Ling, WU Jun-Xiang, LI Yi-Ping. Effects of protease inhibitors on the activities of midgut proteases of larval Grapholitha molesta (Lepidoptera: Tortricidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(10): 1069-1078.
[12] WU Wen-Dan, SUN Hao-Yu, XI Jin-Hui,YIN Jiao, ZHANG Shuai, CAO Ya-Zhong, LI Ke-Bin, XIAO Chun. Observations of the ultrastructure of the fat body and midgut tissues of two white grub species, Holotrichia parallela and H. oblita (Coleoptera: Melolonthidae), infected by entomopathogenic nematode Heterorhabditis bacteriophora[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(8): 836-845.
[13] ZHAO Lü-Quan, WU Hong-Jun, ZHU Dao-Hong. Comparison of digestive capability and digestive enzyme activities in male adults of the wing-dimorphic cricket Velarifictorus ornatus (Orthoptera: Gryllidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(7): 731-738.
[14] TANG Wei-Yuan, XING Cong-Cong, DONG Yong-Gang, WANG Xiao, ZHANG Yi-Ming. Changes in the activities of antioxidant enzymes in Spodoptera litura (Lepidoptera: Noctuidae) larvae infected by the fungus Nomuraea rileyi[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(5): 526-534.
[15] YUAN Rui-Ling,WANG Xiao-Wei,YANG Shan,CHEN Peng. Changes in the activities of enzymes related to energy metabolism in flight muscles of adult Bactrocera dorsalis (Diptera: Tephritidae) at different ages and during tethered flight[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(5): 471-478.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech