Effects of pest insect feeding and mechanical damage on the defensive enzyme activities in leaves at different parts of kidney bean plants
昆虫学报
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (7): 860-870    DOI: 10.16380/j.kcxb.2018.07.013
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Effects of pest insect feeding and mechanical damage on the defensive enzyme activities in leaves at different parts of kidney bean plants
YUE Wen-Bo, ZHI Jun-Rui*, LIU Li, YE Mao, ZHANG Xiang-Qian, ZENG Guang
 (Guizhou Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China)
 Download: PDF (1059 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 This study aims to explore the systematic defense mechanism of bean (Phaseolus vulgaris) plant against different insect pests. 【Methods】 Central leaves of each plant of P. vulgaris were fed by Spodoptera litura, Tetranychus urticae, Frankliniella occidentalis, or pricked manually with an insect needle for 6, 24, 48, 72 and 96 h, respectively, while the upper and lower leaves of each plant were left untreated. The changes in the activities of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) in the upper, central and lower leaves were determined. 【Results】 In the central leaves fed by the three insect species, the activities of POD, CAT and SOD changed significantly, and their changing trends varied with insect species. Compared with the control, the POD activity in central leaves fed by the three insect species increased firstly and then decreased to the level of the control after insect infestation. The POD activity reached the maximum at 24 h after T. urticae infestation, which was 4.6 times as high as the control. However, the POD activity in the central leaves increased obviously and reached the peak at 48 h after both mechanical damage and F. occidentalis infestation. The POD activity in the central leaves ingested by S. litura displayed a slowly increasing trend, and reached the maximum at 72 h after infestation. The changing trends of the CAT activity were different among mechanical damage and feeding of the three insect species. Mechanical damage induced the CAT activity obviously at 24 h after treatment, while all three insect pests caused changes of the CAT activity at earlier time, which rose significantly at 6 h and then changed in different modes with feeding time. In the central leaves, the SOD activity increased significantly only at 96 h after mechanical damage, while T. urticae infestation obviously induced the SOD activity after 24 h. Moreover, the SOD activities were significantly inhibited at 72 h in the leaves fed by S. litura or F. occidentalis. In the undamaged upper and lower leaves, the activities of POD, CAT and SOD also changed significantly after insect infestation or mechanical damage. The POD activities in undamaged upper leaves were inhibited at 96 h. However, the POD activities in lower leaves were induced to different levels. In both upper and lower undamaged leaves, the CAT activities were activated obviously at 24, 6, 6, and 24 h after mechanical damage, the infestation of S. litura, T. urticae and F. occidentalis, respectively. Mechanical damage, and S. litura and T. urticae infestation resulted in prominent increase in the SOD activity in undamaged upper and lower leaves at 96, 72 and 72 h, but F. occidentalis infestation led to the SOD activity being inhibited obviously at 48 and 72 h. 【Conclusion】 Infestation of S. litura, T. urticae and F. occidentalis can induce the activities of defensive enzymes (POD, CAT and SOD) significantly in both damaged central leaves and undamaged upper and lower leaves, which leads to systematic defensive reactions in bean plant, but the changing trends of the enzyme activities vary among treatments. These results indicate that the temporal and spatial effects of the defense are related to the species of insect pests.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
YUE Wen-Bo
ZHI Jun-Rui
LIU Li
YE Mao
ZHANG Xiang-Qian
ZENG Guang-
Key wordsSpodoptera litura   Tetranychus urticae   Frankliniella occidentalis   defensive reaction   temporal and spatial effect   enzyme activity     
Cite this article:   
YUE Wen-Bo,ZHI Jun-Rui,LIU Li et al. Effects of pest insect feeding and mechanical damage on the defensive enzyme activities in leaves at different parts of kidney bean plants[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 860-870.
URL:  
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.07.013      or     http://www.insect.org.cn/EN/Y2018/V61/I7/860
 
No references of article
[1] WU Hai-Bin, GONG Qing-Tao, CHEN Zhen-Zhen, JIANG Li-Li, GONG Yi, XU Yong-Yu, SUN Rui-Hong. Bioefficacy of the combined application of entomopathogenic nematodes and thiamethoxam and its effects on the protective and detoxification enzyme activities in Bradysia odoriphaga (Diptera: Sciaridae) larvae[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 851-859.
[2] SUN Jia-Bin, LIU Nai-Yong, LI Shuang-Mei, YAN Qi, DONG Shuang-Lin. Molecular cloning, tissue expression profiling and binding characterization of the pheromone binding protein SlitPBP4 from Spodoptera litura (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(6): 657-667.
[3] QIAN Xue, WANG Yue-Ying, XIE Huan-Huan, DOU Jie, LI Zhan-Wu, Roman JASHENKO, JI Rong. Effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of Gomphocerus sibiricus (Orthoptera: Acrididae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(5): 499-504.
[4] JIANG Li-Na, Qian-Lei, Xi-Chao, Wang Xue-Li, Yang-Lu, Gui Fu-Rong. Effects of elevated CO2 on the digestive enzyme activities in the adults of Frankliniella occidentalis and F. intonsa (Thysanoptera: Thripidae) on different host plants[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(3): 237-246.
[5] LI Xiao-Wei, SUN Ran-Ran, ZHANG Jin-Ming, ZHANG Zhi-Jun, 吕Yao-Bin . Mating behavior and aggregation pheromone release rhythm of Frankliniella occidentalis (Thysanoptera: Thripidae) adults during photophase[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(12): 1439-1446.
[6] GENG Shuang-Shuang, LI Xiao-Wei, ZHANG Jin-Ming, ZHANG Zhi-Jun, 吕Yao-Bin . Field testing of aggregation pheromones of Frankliniella occidentalis and F. intonsa (Thysanoptera: Thripidae) and their roles in interspecific interaction[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(12): 1447-1456.
[7] TAN Yong-An, ZHAO Xu-Dong, HAO De-Jun, XIAO Liu-Bin, BAI Li-Xin, ZHAO Jing, SUN Yang, JIANG Yi-Ping. Expression, purification and enzymatic characteristics of phosphodiesterase C (AlPLC) of Apolygus lucorum (Hemiptera: Miridae)
 
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(11): 1247-1254.
[8] LIANG Wei-Bo, XIANG Xing, WANG Xue-Gui, YANG Qun-Fang, LUO Jia-Dong. Effects of sublethal doses of thiacloprid on the detoxifying enzyme activities and the growth and reproduction of Spodoptera litura (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(10): 1187-1197.
[9] ZHANG Yu-Yu, Zhi Jun-Rui, Liu Yong, Ye Mao. Frankliniella occidentalis (Thysanoptera: Thripidae) infestation on the expression of defensive genes in leaves at different parts of kidney bean plant[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(1): 1-8.
[10] FAN Lin-Fang, HE Wen-Jing, SU Zhi-Ren, ZHU Jia-Ming, LUO Wei, ZHANG Chun, HUANG Li-Hua. Construction of yeast two-hybrid system and its application in screening the interacting proteins of ecdysone receptors in Spodoptera litura (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(8): 801-811.
[11] GUO Cheng-Lin, QIN Liu-Yan, WAN Shu-Qing, MA Yue-Feng, QIN Jian-Lin, MA Yong-Lin. Insecticidal activity and identification of the active ingredients of Clausena lansium seed extracts against larval Spodoptera litura (Lepidoptera: Noctuidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(8): 839-845.
[12] ZHI Jun-Rui, TIAN Tian, WEN Juan, LIU Yong. Effects of kidney bean damaged by Frankliniella occidentalis (Thysanoptera: Thripidae) or Tetranychus urticae (Acari: Tetranychidae) on the activities of protective and detoxification enzymes in the other subsequent herbivore of both[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(7): 707-715.
[13] HAO Qiang, HUANG Qian, LIANG Wei-Bo, GONG Chang-Wei, WANG Xue-Gui. Age-stage two-sex life tables of Spodoptera litura (Lepidoptera: Noctuidae) at different temperatures[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(6): 654-662.
[14] JIN Gui-Hua, GONG Ya-Jun, QIAN Zong-Wei, ZHU Liang, WANG Ze-Hua, CHEN Jin-Cui, WEI Shu-Jun. Selectivity and fitness of the two-spotted spider mite, Tetranychus urticae (Acarina: Tetranychidae) to different varieties of eggplant[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(3): 328-336.
[15] MA Ya-Bin, SUN Li-Juan, LI Hong-Gang, WANG Si-Fang, ZHANG Bin, ZHENG Chang-Ying, WAN Fang-Hao1. Effects of high temperature on ovary development and yolk protein content of Frankliniella occidentalis (Thysanoptera: Thripidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 127-137.
Copyright © 2011 ACTA ENTOMOLOGICA SINICA
Supported by:Beijing Magtech