Histopathological observation of the integument and fat body of <em>Antheraea pernyi</em> (Lepidoptera: Saturniidae) pupae infected by <em>Beauveria bassiana</em>
       Home | About Journal | Editorial Board | Subscription | Instruction | Advertisement | Contact Us | Chinese
ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (8): 923-931    DOI: 10.16380/j.kcxb.2018.08.005
RESEARCH PAPERS Current Issue | Next Issue | Archive | Adv Search Previous Articles  |  Next Articles  
Histopathological observation of the integument and fat body of Antheraea pernyi (Lepidoptera: Saturniidae) pupae infected by Beauveria bassiana
SHEN Yu1, AN Li-Jia1, LI Wen-Li2,*  
(1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China; 2. School of Life and Medicine, Dalian University of Technology, Panjin, Liaoning 124221, China)
 Download: PDF (6055 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 【Aim】 The purpose of this study is to investigate the infection process by Beauveria bassiana in the integument and fat body of Antheraea pernyi pupae so as to further clarify the pathogenesis in A. pernyi pupae infected by B. bassiana. 【Methods】 The morphological changes in the integument and fat body of A. pernyi pupae infected by B. bassiana were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. 【Results】 SEM observation indicated that the infection of B. bassiana in A. pernyi pupae mainly occurred at valves, bristles root and the intersegmental membrane between head and thorax. Besides, conidia penetrated into the body directly from the thick and hard wall of the body. TEM observation indicated that in the fat body, organelles except mitochondria were first infected, and then the fat droplets were eroded. The infected fat droplets became shallow, their surface had many holes and became deformed, and the fat droplets were divided into small fat droplets, indicating that they were completely eroded. When pupae were rigid, fat cells were completely destroyed, leaving only the blastospores and hyphae. 【Conclusion】 The integument and fat body of A. pernyi pupae infected by B. bassiana have obvious histopathological changes.
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
AN Li-Jia
LI Wen-Li
Key wordsBeauveria bassiana   Antheraea pernyi   pupae   scanning electron microscopy (SEM)   transmission electron microscopy (TEM)   infection process     
Cite this article:   
SHEN Yu,AN Li-Jia,LI Wen-Li. Histopathological observation of the integument and fat body of Antheraea pernyi (Lepidoptera: Saturniidae) pupae infected by Beauveria bassiana[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(8): 923-931.
http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.08.005      or     http://www.insect.org.cn/EN/Y2018/V61/I8/923
No references of article
[1] WANG De-Yi, RU Yu-Tao, WANG Yong, MA Yue-Yue, NA Shuang, SUN Liang-Zhen, JIANG Yi-Ren, QIN Li. Gene expression patterns and activities of trehalases in Antheraea pernyi (Lepidoptera: Saturniidae) pupae during diapause and diapause termination[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(7): 784-794.
[2] MA Yue-Yue, LIU Wei, WANG Yong, WANG De-Yi, LEI Piao, RU Yu-Tao, JIANG Yi-Ren, YANG Rui-Sheng, QIN Li. Expression profiling and functional analysis of RR-1 cuticular protein genes ApCP12 and ApCP23 in Antheraea pernyi (Lepidoptera: Saturniidae)[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(2): 188-199.
[3] ZHONG Jian, LIU Zeng-Hu, YANG Wei-Ke, ZHU Feng, DONG Zhan-Peng. Sequencing and analysis of the complete mitochondrial genome of Antheraea assama (Lepidoptera: Saturniidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(8): 936-949.
[4] ZHONG Hai-Ying, ZHANG Ya-Lin, WEI Cong. Comparative morphology and ultrastructure of Malpighian tubules of the mute cicada Karenia caelatata and the coleopterous spittlebug Lepyronia coleoptrata
(Hemiptera: Cicadomorpha) (In English)
[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(7): 825-834.
[5] HUANG Wei-Ya, Li-Li. Maternal care improves offspring developmental performance in Scleroderma guani (Hymenoptera: Bethylidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(4): 441-449.
[6] WANG He, Wang-Hui, Wang Wei-Jie, Li-Jia, Zhao Wen-Ai, Qi Li-Li. Pupal morphogenesis of Lucilia cuprina (Diptera: Calliphoridae) under natural conditions and its significance in forensic medicine[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(2): 222-228.
[7] YANG Qing-Peng, Li Li. Negative influence of Beauveria bassiana infection on female adults of a cryptoparasitoid Scleroderma guani (Hymenoptera: Bethylidae)[J]. ACTA ENTOMOLOGICA SINICA, 2017, 60(1): 53-59.
[8] YU Qin, WANG Fang, ZHANG Run-Xiang, GUO Gui-Ming, FAN Ren-Jun, HAO Chi. Effects of pupal weight on the fecundity and longevity of adults and the larval development of the next generation in Grapholitha molesta (Lepidoptera: Tortricidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 985-990.
[9] JIANG Lu, HUA Bao-Zhen. Morphology of the immature stages of Panorpa macrostyla  Hua (Mecoptera: Panorpidae) with notes on its biology[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 1004-1012.
[10] HUANG Ling, SUN Liang-Zhen, WANG Yong, RU Yu-Tao, Muhammad IRFAN, JIANG Yi-Ren, Shi-Sheng-Lin , YANG Rui-Sheng, LI Xi-Sheng, QIN Li. Changes in the expression of trehalose-6-phosphate synthase gene in Antheraea pernyi (Lepidoptera: Saturniidae) during pupal diapause termination[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(9): 938-947.
[11] FU Ying-Ying, MA Yu-Kun, REN Shu-Wen, YANG Li-Jun, LIU Chun-Guang, FU Hai-Yan, HOU Yue-Ying, YANG Feng-Shan. Changes in physiological indices of Antheraea pernyi (Lepidoptera: Saturniidae) pupae induced by gram-positive bacteria  Bacillus thuringeinsis and gramnegative bacteria Escherichia coli[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(2): 192-199.
[12] XIANG Yu-Yong, KONG Dan-Dan, LIU Tong-Xian, ZHANG Shi-Ze. Observation of the sensilla on female adult antenna and larval head of Agrotis ypsilon (Lepidoptera: Noctuidae) with scanning electron microscope[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(12): 1340-1347.
[13] ZHANG Long-Wa, KANG Ke, LIU Yu-Jun, ZHANG Jing, SUN Long, ZHAN Cheng, HUANG Chang-Chun, JIANG Li-Ya, YE Kai-Yun, DING De-Gui. Evaluation of Beauveria bassiana isolates as potential agents for control of Hyphantria cunea (Lepidoptera: Arctiidae)[J]. ACTA ENTOMOLOGICA SINICA, 2016, 59(1): 111-118.
[14] LI Pei-Guang, QIU Shi-Qi, YE Bao-Hua, WANG Ning-Xin, HUANG Da-Wei. Influence of removing Wolbachia on the reproductive fitness and adult longevity of Nasonia vitripennis (Hymenoptera: Pteromalidae)[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(9): 966-972.
[15] HE Qiang, LIU Shao-Lun, MA Zheng-Gang, ZHANG You-Yi, Charles R. VOSSBRIN. Comparative genomics of three geographical strains reveals genetic diversity in Antheraea pernyi nucleopolyhedrovirus (AnpeNPV)(In English[J]. ACTA ENTOMOLOGICA SINICA, 2015, 58(5): 535-545.
Supported by:Beijing Magtech