Histopathological observation of the integument and fat body of <em>Antheraea pernyi</em> (Lepidoptera: Saturniidae) pupae infected by <em>Beauveria bassiana</em>
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ACTA ENTOMOLOGICA SINICA  2018, Vol. 61 Issue (8): 923-931    DOI: 10.16380/j.kcxb.2018.08.005
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Histopathological observation of the integument and fat body of Antheraea pernyi (Lepidoptera: Saturniidae) pupae infected by Beauveria bassiana
SHEN Yu1, AN Li-Jia1, LI Wen-Li2,*  
(1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China; 2. School of Life and Medicine, Dalian University of Technology, Panjin, Liaoning 124221, China)
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Abstract 【Aim】 The purpose of this study is to investigate the infection process by Beauveria bassiana in the integument and fat body of Antheraea pernyi pupae so as to further clarify the pathogenesis in A. pernyi pupae infected by B. bassiana. 【Methods】 The morphological changes in the integument and fat body of A. pernyi pupae infected by B. bassiana were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. 【Results】 SEM observation indicated that the infection of B. bassiana in A. pernyi pupae mainly occurred at valves, bristles root and the intersegmental membrane between head and thorax. Besides, conidia penetrated into the body directly from the thick and hard wall of the body. TEM observation indicated that in the fat body, organelles except mitochondria were first infected, and then the fat droplets were eroded. The infected fat droplets became shallow, their surface had many holes and became deformed, and the fat droplets were divided into small fat droplets, indicating that they were completely eroded. When pupae were rigid, fat cells were completely destroyed, leaving only the blastospores and hyphae. 【Conclusion】 The integument and fat body of A. pernyi pupae infected by B. bassiana have obvious histopathological changes.
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SHEN Yu
AN Li-Jia
LI Wen-Li
Key wordsBeauveria bassiana   Antheraea pernyi   pupae   scanning electron microscopy (SEM)   transmission electron microscopy (TEM)   infection process     
Cite this article:   
SHEN Yu,AN Li-Jia,LI Wen-Li. Histopathological observation of the integument and fat body of Antheraea pernyi (Lepidoptera: Saturniidae) pupae infected by Beauveria bassiana[J]. ACTA ENTOMOLOGICA SINICA, 2018, 61(8): 923-931.
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http://www.insect.org.cn/EN/10.16380/j.kcxb.2018.08.005      or     http://www.insect.org.cn/EN/Y2018/V61/I8/923
 
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