昆虫学报 ›› 2016, Vol. 59 ›› Issue (2): 200-208.doi: 10.16380/j.kcxb.2016.02.010

• 研究论文 • 上一篇    下一篇

基于16S rDNA基因序列的泽兰实蝇幼虫肠道细菌多样性分析

张某1, 杨璞2, 朱家颖3, 袁远1, 桂富荣1, 高熹1,*, 吴国星1,*   

  1. （1. 云南农业大学植物保护学院, 昆明 650201; 2. 中国林业科学研究院资源昆虫研究所, 国家林业局资源昆虫培育与利用重点实验室, 昆明 650224; 3. 西南林业大学云南省森林灾害预警与控制重点实验室, 昆明 650224）
  • 出版日期:2016-02-20 发布日期:2016-02-20
  • 作者简介:张某, 男, 1989年8月生, 贵州毕节人, 硕士研究生, 研究方向为有害生物治理, E-mail: zhangmengtao01@outlook.com

Analysis of the bacterial diversity in the intestine of larval Procecidochares utilis (Diptera: Trypetidae) based on 16S rDNA gene sequence

ZHANG Mou1, YANG Pu2, ZHU Jia-Ying3, YUAN Yuan1, GUI Fu-Rong1, GAO Xi1,*, WU Guo-Xing1,*   

  1. (1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China; 3. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China)
  • Online:2016-02-20 Published:2016-02-20

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摘要： 【目的】探究泽兰实蝇 Procecidochares utilis 幼虫肠道细菌的多样性。【方法】利用Illumina HiSeq技术对泽兰实蝇幼虫肠道细菌的16S rDNA-V6变异区序列进行测序，应用USEARCH和QIIME等软件整理和统计样品序列数目和操作分类单元（operational taxonomic unit, OTU）数量，分析物种的丰度和Alpha多样性。【结果】共获得1 593 506 对reads，拼接为1 579 372条tags，经过滤后得到的1 572 860条tags聚类为1 341个OTU。总共注释到13个门，4个纲，6个目，7个科，10个属和4个种。其中变形菌门(Proteobacteria)的细菌为优势菌，占99%；在属分类阶元上，沃尔巴克氏体属 Wolbachia 占45%，是优势属。【结论】泽兰实蝇幼虫肠道细菌多样性丰富。相关的种类和丰度信息为后期研究揭示肠道细菌介导泽兰实蝇寄主植物专化性奠定了基础。

关键词: 泽兰实蝇, 肠道细菌, 多样性, 16S rDNA, Illumina

Abstract: 【Aim】 To clarify the bacterial diversity in the intestine of larval  Procecidochares utilis. 【Methods】 The V6 regions of the 16S rDNA genes of intestinal bacteria in  P. utilis larvae was sequenced by Illumina HiSeq techniques. The numbers of sequences and operational taxonomic units (OTUs), species abundance and alpha diversity in samples were analyzed using USEARCH and QIIME softwares. 【Results】 A total of 1 593 506 reads were obtained, which were combined to 1 579 372 tags. After filtration, 1 572 860 tags were clustered into 1 341 OTUs. They were annotated into 13 phyla, 4 classes, 6 orders, 7 families, 10 genera, and 4 species. The Proteobacteria was the most dominant, accounting for 99%. At the genus level, Wolbachia was the dominant bacteria(45%). 【Conclusion】 The results show that the bacteria in the intestine of larval P. utilis are diverse. The information of species richness will lay a foundation for further research on the association of the intestinal bacteria with host plant to reveal feeding specialization in P. utilis.

Key words: Procecidochares utilis, intestinal bacteria, diversity, 16S rDNA, Illumina

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