›› 2018, Vol. 61 ›› Issue (6): 676-685.doi: 10.16380/j.kcxb.2018.06.006

• 研究论文 • 上一篇    下一篇

类肠膜魏斯氏菌通过调节蜕皮激素和胰岛素通路促进黑腹果蝇生长发育(英文)

李恩惠1, 王晓阳3, 张乐宵3, 白芃3, 赵欣1, 刘威2,*, 张策1,*   

  1.  (1. 山西医科大学生理学系, 太原 030001; 2. 山西医科大学汾阳学院医学检验系, 山西汾阳 032200; 3. 山西医科大学汾阳学院基础医学院, 山西汾阳 032200)
  • 出版日期:2018-06-20 发布日期:2018-06-20

Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)

LI En-Hui1, WANG Xiao-Yang3, ZHANG Yue-Xiao3, BAI Peng3, ZHAO Xin1, LIU Wei2,*, ZHANG Ce1,*   

  1. (1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China; 3. Department of Basic Medical, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China)
  • Online:2018-06-20 Published:2018-06-20

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摘要： 【目的】本研究旨在揭示果蝇乳酸菌类肠膜魏斯氏菌Weisellas paramesenteroides对黑腹果蝇Drosophila melanogaster生长发育的影响。【方法】利用选择性培养基分离黑腹果蝇成虫肠道乳酸菌，通过16S rRNA基因序列比对鉴定菌株。通过统计从卵至蛹化和羽化的时间检测果蝇发育历期,并以幼虫体表面积为指标检测果蝇生长速率。利用qPCR检测产卵后不同时间生长激素信号通路基因（dib, E74B和PTTH）及胰岛素信号通路基因（DILP2, DILP3和InR）的表达。通过葡萄糖氧化酶法检测3龄幼虫血淋巴中的葡萄糖水平。【结果】从黑腹果蝇成虫肠道内分离到类肠膜魏斯氏菌，并可以在果蝇肠道内有效定殖。类肠膜魏斯氏菌通过提高果蝇生长速率缩短果蝇卵至蛹化和羽化的时间。qPCR结果显示，类肠膜魏斯氏菌增加了dib, E74B和PTTH的表达量，同时增加了DILP2和DILP3的表达量，降低了InR表达量和幼虫血淋巴中的葡萄糖水平。【结论】类肠膜魏斯氏菌是黑腹果蝇的一种共生菌，通过激活蜕皮激素和胰岛素信号通路，促进生长发育。

关键词:  类肠膜魏斯氏菌, 黑腹果蝇, 生长发育, 蜕皮激素信号通路, 胰岛素信号通路

Abstract: 【Aim】 To investigate the effects of lactic acid bacteria Weisellas paramesenteroides on the growth and development of Drosophila melanogaster. 【Methods】 Bacterium was isolated from the gut of D.melanogaster adults with selective medium and identified by BLAST analysis of 16S rRNA gene. The developmeantal duration of D. melanogaster was quantified by counting the time to puparium formation and adult eclosion, and the growth rate was calculated as the body surface area of larvae. The expression levels of genes involved in hormoral (dib, E74B and PTTH) and insulin signaling（DILP2, DILP3 and InR）of D. melanogaster at different time after egg deposition were assayed by quantitative real-time PCR. The glucose level in the hemolymph of the 3rd instar larvae was measured by glucose oxidase assay. 【Results】 W. paramesenteroides was isoloated and efficiently colonized in the gut of D. melanogaster adults. W. paramesenteroides shortened the time to puparium formation and adult eclosion of D. melanogaster by enhancing the growth rate of flies. W. paramesenteroides increased the expression levels of dib, E74B and PTTH, as well as the expression of DILP2 and DILP3, while decreased the expression levels of InR and the glucose level in larval hemolymph.【Conclusion】 W. paramesenteroides is the symbiotic bacterium of D. melanogaster, and promotes its development by activting the ecdysone and insulin signaling pathways.

Key words: Weissella paramesenteroides, Drosophila melanogaster, development, ecdysone signaling pathway, insulin signaling pathway

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