关键词: 红颈常室茧蜂, 滞育蛹, 冷藏, 温度, 相对湿度, 储存历期

Abstract: 【Aim】Peristenus spretus is an important parasitoid of mirid bugs, with a strong parasitic ability against the nymphs of Apolygus lucorum. This study aims to determine the suitable temperature and humidity conditions for storage of the diapause pupae of P. spretus, and to identify the optimal storage duration. 【Methods】The emergence time, sex ratio, emergence rate, longevity and number of eggs laid of P. spretus adults were observed under different temperatures (5 and 10 ℃), relative humidity (RH)(40%, 60% and 80%) and storage duration (90, 120, 150 and 180 d) to clarify the optimal storage environmental conditions and storage duration of the diapause pupae of P. spretus. 【Results】Under the constant 60% RH, at 5 ℃, the emergence rate of P. spretus was 60.56%, the male and female adult longevity was 12.73 and 12.67 d, respectively, and the number of eggs laid per female was 199.86, which were higher than the results observed at 10 ℃ (47.22%, 7.23 d, 8.38 d and 62.14, respectively). Under the storage conditions of the temperature 5 ℃ and different RH, the highest adult emergence rate (52.77%) was observed at 60% RH, where female adults exhibited longer longevity (12.40 d) and higher fecundity (133.86 eggs/female). Under the storage condition of 5 ℃ and 60% RH, the diapause pupae stored for 120 or 150 d maintained an adult emergence rate above 60%. After the diapause pupae were stored under the condition of 5 ℃ and 60% RH for 120 and 150 d, the male adult longevity was 12.73 and 8.90 d, respectively, and the female adult longevity was 12.47 and 14.43 d, respectively, while the numbers of eggs laid per female were 199.86 and 169.71, respectively. After 180-d storage of diapause pupae under the condition of 5 ℃ and 60% RH, the adult emergence rate declined to 48.33%, significantly lower than that of the control group (non-diapause pupae)(68.89%).【Conclusion】In conclusion, the optimal storage duration for the diapause pupae of P. spretus was 120 d or 150 d under the storage condition of 5 ℃ and 60% RH. These findings provide crucial parameters for the long-term storage, and lay the foundation for the standardized and large-scale production of P. spretus.

Key words: Peristenus spretus, diapause pupae, cold storage, temperature, relative humidity, storage duration