青蜂总科三种寄生蜂的气味结合蛋白和化学感受蛋白基因家族的全基因组鉴定及进化分析

doi:10.16380/j.kcxb.2025.12.011

昆虫学报 ›› 2025, Vol. 68 ›› Issue (12): 1736-1749.doi: 10.16380/j.kcxb.2025.12.011

青蜂总科三种寄生蜂的气味结合蛋白和化学感受蛋白基因家族的全基因组鉴定及进化分析

朱登辉^1,#, 吕卉^3,#, 吴晋渝¹, 靳雯婷¹, 潘雨飞¹, 吴奉员¹, 李景浩⁴, 何树林^1,2,*, 李飞^1,2,*

(1. 重庆师范大学生命科学学院, 重庆 401331; 2. 重庆师范大学, 重庆媒介生物控制和利用重点实验室, 重庆 401331; 3. 重庆中医药学院中药学院, 重庆 402760; 4. 国家林业和草原局生物灾害防控中心, 沈阳 110034)

出版日期:2025-12-20 发布日期:2026-01-29

Genome-wide identification and evolutionary analysis of odorant-binding protein and chemosensory protein gene families in three parasitoid species of Chrysidoidea (Insecta: Hymenoptera)

ZHU Deng-Hui^1,#, LÜ Hui^3,#, WU Jin-Yu¹, JIN Wen-Ting¹, PAN Yu-Fei¹, WU Feng-Yuan¹, LI Jing-Hao⁴, HE Shu-Lin^1,2,*, LI Fei^1,2,*

(1. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China; 2. Chongqing Key Laboratory of Vector Control and Utilization, Chongqing Normal University, Chongqing 401331, China; 3. School of Traditional Chinese Pharmacy, Chongqing University of Chinese Medicine, Chongqing 402760, China; 4. Center for Biological Disaster Prevention and Control, National Forestry and Grassland Administration, Shenyang 110034, China)

Online:2025-12-20 Published:2026-01-29

摘要/Abstract

摘要： 【目的】在全基因组水平上对青蜂总科(Chrysidoidea) 3种寄生蜂的气味结合蛋白(odorant-binding proteins, OBPs)和化学感受蛋白(chemosensory proteins, CSPs)家族基因进行鉴定及进化分析，预测其家族成员可能的生物学功能，以阐明其在化学感知机制及潜在社会行为演化中的可能关联。【方法】从公开数据库中筛选青蜂总科3种寄生蜂(勒氏棱角肿腿蜂Goniozus legneri、松褐天牛肿腿蜂Sclerodermus alternatusi和黄腿双距螯蜂Gonatopus flavifemur)以及2个参考物种意大利蜜蜂Apis mellifera ligustica和实蝇茧蜂Diachasma alloeum的基因组数据。通过blastp和interproscan方法鉴定OBP和CSP家族基因，使用生物信息学方法(Expert Protein Analysis System, SignalP, DeepTMHMM, WoLF PSORT和MEME)预测OBPs和CSPs的理化性质和保守基序及其基因的染色体定位；采用IQ-TREE程序运用最大似然法构建OBPs和CSPs的系统发育树；利用MCScanX推测基因共线性和复制模式。【结果】勒氏棱角肿腿蜂、松褐天牛肿腿蜂和黄腿双距螯蜂基因组中共鉴定到9~13个OBP基因和5~11个CSP基因，其中黄腿双距螯蜂基因组中的OBP和CSP基因最少，为9个OBP基因和5个CSP基因；绝大多数OBPs均含有基序1和基序2，并且基序的顺序为(3)-1-(4)/(5)-2；所有的CSPs都含有基序1和基序2，基序的顺序为1-2-(3)/(4)。这5种膜翅目(Hymenoptera)昆虫的OBPs和CSPs均划分为8个分支。且这5种膜翅目昆虫的OBP和CSP基因处于共线性区块的较少，仅有6对OBP基因处于共线性区块，有8对CSP基因处于共线性区块。基因复制分析结果显示物种特异性复制模式：黄腿双距螯蜂和勒氏棱角肿腿蜂中OBP基因主要以非重复为主，松褐天牛肿腿蜂、意大利蜜蜂和实蝇茧蜂中OBP基因以串联重复为主；而CSP基因以分散重复和串联重复为主。【结论】本研究为青蜂总科3种寄生蜂OBP和CSP家族基因提供了信息框架，丰富了青蜂总科基因组数据库，为后续青蜂总科社会行为演化的研究提供了科学参考。

关键词: 青蜂总科, 气味结合蛋白, 化学感受蛋白, 全基因组鉴定, 复制模式, 系统发育, 社会行为

Abstract: 【Aim】 To perform genome-wide identification and evolutionary analysis of genes of the odorant-binding protein (OBP) and chemosensory protein (CSP) families in three parasitoid species of the superfamily Chrysidoidea, and to predict the potential biological functions of the members of these families, so as to explore their possible roles in chemosensory mechanisms and the evolution of social behavior. 【Methods】 The genomic data of three Chrysidoidea parasitoid species Goniozus legneri, Sclerodermus alternatusi and Gonatopus flavifemur, as well as two reference species (Apis mellifera ligustica and Diachasma alloeum) were retrieved from public databases. The OBP and CSP family genes were identified using blastp and interproscan. The physicochemical properties and conserved motifs of OBPs and CSPs and their gene chromosomal locations were predicted using bioinformatic tools (Expert Protein Analysis System, SignalP, DeepTMHMM, WoLF PSORT and MEME). The phylogenetic trees of OBPs and CSPs were constructed using the maximum likelihood method with IQ-TREE. Gene synteny and duplication patterns were analyzed using MCScanX. 【Results】 A total of 9－13 OBP genes and 5－11 CSP genes were identified in the genomes of Goniozus legneri, Sclerodermus alternatusi and Gonatopus flavifemur. Gonatopus flavifemur had the fewest OBP and CSP genes (9 and 5, respectively). Most OBPs contained motif 1 and motif 2, typically in the order of (3)-1-(4)/(5)-2. All CSPs had motif 1 and motif 2 arranged in the order 1-2-(3)/(4). The OBP and CSP genes of the five hymenopteran species were clustered into eight phylogenetic clades. Few OBP and CSP genes (only 6 OBP and 8 CSP gene pairs) of the five hymenopteran species were located in collinear blocks. Gene duplication analysis result showed species-specific duplication modes: Singleton dominated in OBP genes of Gonatopus flavifemur and Goniozus legneri, while tandem repeats were predominant in OBP genes of the other species. For CSP genes, dispersed and tandem repeats were the most common. 【Conclusion】 The study provides an information frame of the OBP and CSP gene families in three parasitoid species of the superfamily Chrysidoidea, enriching the genomic database of this superfamily and providing a scientific reference for future research into the evolution of social behavior of Chrysidoidea.

Key words: Chrysidoidea, odorant-binding proteins, chemosensory proteins, genome-wide identification, duplication modes, phylogeny, social behavior

朱登辉, 吕卉, 吴晋渝, 靳雯婷, 潘雨飞, 吴奉员, 李景浩, 何树林, 李飞. 青蜂总科三种寄生蜂的气味结合蛋白和化学感受蛋白基因家族的全基因组鉴定及进化分析[J]. 昆虫学报, 2025, 68(12): 1736-1749.

ZHU Deng-Hui, LÜ Hui, WU Jin-Yu, JIN Wen-Ting, PAN Yu-Fei, WU Feng-Yuan, LI Jing-Hao, HE Shu-Lin, LI Fei. Genome-wide identification and evolutionary analysis of odorant-binding protein and chemosensory protein gene families in three parasitoid species of Chrysidoidea (Insecta: Hymenoptera)[J]. Acta Entomologica Sinica, 2025, 68(12): 1736-1749.

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青蜂总科三种寄生蜂的气味结合蛋白和化学感受蛋白基因家族的全基因组鉴定及进化分析

Genome-wide identification and evolutionary analysis of odorant-binding protein and chemosensory protein gene families in three parasitoid species of Chrysidoidea (Insecta: Hymenoptera)

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