›› 2013, Vol. 56 ›› Issue (10): 1229-1234.

• 简报 • 上一篇    

伊朗棉铃虫核型多角体病毒三个分离株在规模化生产中的产量参数 (英文)

Ali MEHRVAR   

  1. (Department of Plant Protection, Faculty of Agriculture, University of Maragheh, Maragheh, EastAzarbaijan, Iran)
  • 出版日期:2013-10-20 发布日期:2013-10-20

Virus yield parameters in mass production of three Iranian geographic isolates of Helicoverpa armigera nucleopolyhedrovirus (In English)

Ali MEHRVAR   

  1. (Department of Plant Protection, Faculty of Agriculture, University of Maragheh, Maragheh, EastAzarbaijan, Iran)
  • Online:2013-10-20 Published:2013-10-20

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摘要： 对采自伊朗阿塞拜疆东部西红柿大田的棉铃虫Helicoverpa armigera核型多角体病毒3个分离株［Maragheh (MRG), Nebrin (NBN)和 Marand (MRD)］繁殖的3个主要参数（幼虫期、 接种剂量和培养温度）进行了研究， 以在室内条件下筛选很有前景的分离株。在测试的各龄幼虫中， 5龄初期幼虫的病毒产量最高。最适接种剂量和接种温度分别为1 965.87 OB/mm2 和25℃。而在所有测试中， 采自Nebrin 的NBN分离株在规模化生产中表现最佳。

关键词:  , 棉铃虫, 核型多角体病毒, 规模化生产, 产量参数, 接种剂量, 培养温度

Abstract:  Three main parameters of virus propagation, viz., larval stage, inoculation dose and incubation temperature were studied in three nucleopolyhedrovirus isolates of Helicoverpa armigera, i. e., Maragheh (MRG), Nebrin (NBN), and Marand (MRD), collected from tomato fields of East-Azarbaijan, Iran, to select the highly promising isolate under laboratory conditions. Among the larval stages evaluated, the early 5th instar larvae recorded the maximum virus yield. The optimum inoculation dose as well as incubation temperature have been determined as 1 965.87 OB/mm2 and 25℃, respectively. However, the NBN isolate collected from Nebrin recorded the optimum mass production behavior in all the assays.

Key words: Helicoverpa armigera, nucleopolyhedrovirus, mass production, yield parameters, inoculation dose, inoculation temperature

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