Abstract: 【Aim】 This study aims to explore the volatiles from the host plant Juglans mandshurica, which affect the behavioral response of the brown mulberry longhorn beetle Apriona germari, and to provide a theoretical basis for screening plant-originated attractants or repellents of this insect. 【Methods】 Volatiles released from J. mandshurica in different damaged states ［healthy (CK), and feeding, oviposition and boring damage by A. germari］ were collected by using dynamic headspace adsorption, and those causing electrophysiological and behavioral responses in A. germari were identified by gas chromatography-mass spectrometry (GC-MS), gas chromatography-electrophysiological antennal detecting system (GC-EAD) and Y-tube olfactometer. 【Results】 In different damaged states, the main volatiles released from J. mandshurica included terpenes and aromatic compounds, and significant differences existed in the relative contents of several components of them. There were one or several unique volatile components in each damaged state. However, A. germari adults only had significant electrophysiological responses to six volatiles, including (1R)-(+)-alpha-pinene, cis-3-hexenyl acetate, nonanal, α-terpineol, 2-ethylhexyl acrylate and hexadecane, but had no responses to the other volatiles. Female adults had the strongest electrophysiological responses to nonanal, while male adults had the strongest electrophysiological responses to cis-3-hexenyl acetate. The olfactory reaction showed that 2-ethylhexyl acrylate had a significant attractivity to the female adults of A. germari (P＜0.05), while cis-3-hexenyl acetate had an extremely significant attractivity to the male adults (P<0.01). And nonanal had a significant attractivity to both female and male adults (P＜0.05), while α-terpineol had a significant repellent activity to them (P＜0.05). 【Conclusion】 These results indicate that cis-3-hexenyl acetate, nonanal and 2-ethylhexyl acrylate show strong attractivity to A. germari adults, while α-terpineol shows good repellent activity.

Key words: Apriona germari; Juglans mandshurica, volatiles, GC-MS, GC-EAD, Y-tube olfactometer