Abstract: 【Aim】 To clarify the effects of adding glucose to foods on the development and gut bacterial community structure of Drosophila suzukii, and select the gut bacteria related to glycometabolism of D. suzulii. 【Methods】 After being fed with the artificial diets added with different concentrations of glucose (0, 5% and 10%), the growth and development of axenic (gut microbiota-free) and conventionally reared strains of D. suzukii, and the changes in the glucose content were observed, and the diversity of gut bacteria of conventionally reared D. suzukii adults was analyzed by PacBio sequencing platform. 【Results】 In the 5% glucose treatment group, the larval survival rate and adult eclosion rate of conventionally reared D. suzukii increased by 60.44% and 123.79%, respectively, and in the 10% glucose treatment group, the larval survival rate of conventionally reared D. suzukii increased by 87.87%, as compared to those in the control group. However, the development of axenic D. suzukii was not significantly affected by the addition of glucose. Axenic D. suzukii adults could not utilize glucose, leading to their higher glucose content than conventionally reared adults. The diversity analysis of gut bacteria revealed that Gluconobacter and Acetobacter were the dominant genera of gut bacteria in conventionally reared D. suzukii adults. Glucose added in foods significantly increased the diversity indexes of gut bacteria and the abundance of Acetobacter, Providencia and Morganella. The dominant species in the gut of conventionally reared D. suzukii adults were L. lactis, G. frateurii, A. thailandicus and P. alcalifaciens, and their relative abundance in D. suzukii adults exposed to different concentrations of glucose was significantly different. 【Conclusion】 Axenic D. suzukii can not utilize glucose directly. Gut bacteria (mainly Acetobacter) can promote the utilization of glucose in D. suzukii. These results provide an important basis for studying the mechanism of gut bacteria involved in nutritional metabolism in D. suzukii.

Key words: Drosophila suzukii, glucose, yeast, growth and development, gut bacteria, glycometabolism