Abstract: 【Aim】 The phylogenetic relationships among higher taxonomic groups (family or superfamily) within Cimicomorpha were reconstructed using genome and transcriptome data, with the aim of providing data to further clarify higher-level phylogenetic relationships within Cimicomorpha. 【Methods】 Through high-throughput sequencing technology, we successfully obtained the genome data of Stephanotis nashi and integrated them with publicly available genome and transcriptome data from 38 other species of Heteroptera. Using the software BUSCO, we extracted single-copy orthologous genes from the above species, and constructed six data matrices of varying completeness to study the higher-level phylogenetic relationships within Cimicomorpha. 【Results】 The number of single-copy orthologous genes in Cimicomorpha ranged from 397 to 2 437. In Cimicomorpha, phylogenetic analyses consistently supported the monophyly of Reduvioidea, Naboidea, Cimicoidea, Tingoidea, and Miroidea. The Reduvioidea group formed an independent clade and was sister to a clade consisting of Naboidea, Cimicoidea, Tingoidea, and Miroidea. Additionally, at the family level, the phylogenetic analysis indicated that the families Reduviidae, Nabidae, Anthocoridae, Tingidae and Miridae each represented a monophyletic group, and the families Tingidae and Miridae were sister groups. 【Conclusion】 The results of this study demonstrate the practicality of using genome and transcriptome data in constructing the phylogenetic relationships within Cimicomorpha, and also provide genome and transcriptome data to further understand the higher-level phylogenetic relationships in Cimicomorpha.

Key words: Hemiptera, Cimicomorpha, genome, transcriptome, single-copy orthologous gene, phylogeny