【Aim】 To develop a genetically modified Anopheles sinensis line capable of transcribing Cas9 specifically in the integument during pupal to adult stages, using the promoter of the highly expressed cuticular protein gene AsCPR75 during the late pupal to adult stages as a regulatory element. 【Methods】 Based on transcriptomic datasets derived from multiple tissues of An. sinensis, including midgut, Malpighian tubules, ovary, fat body, antennae, salivary gland, testes and integument, cuticular protein genes with high expression levels in the integument were systematically screened. qPCR was used to validate the expression levels of candidate genes across different developmental stages (egg, 1st－4th instar larvae, pupa and adult) as well as in the adult head-thorax and abdomen. A 4 000 bp sequence upstream of the translational start site of target gene was selected as a putative promoter region to construct a transgenic plasmid capable of conditionally driving Cas9 transcription, and the plasmid was introduced into the female adult of An. sinensis via embryo microinjection. Transgenic progeny was screened based on fluorescent markers, and the genomic insertion sites of the transgene cassette in positive individuals were identified using inverse PCR. qPCR was employed to examine the expression levels of Cas9 in the transgenic lines at different developmental stages (pupa and adult) and in the adult integument at 0 h after emergence. 【Results】 A cuticular protein gene AsCPR75 highly expressed in the integument from late pupal to adult stages of An. sinensis was screened. qPCR analysis confirmed that the AsCPR75 expression was upregulated from 24 h during the pupal stage and reached its highest level at 0 h in adult stage. AsCPR75 was highly expressed in the adult head-thorax and abdomen, with significantly higher expression level in the abdominal integument than that in the other abdominal parts. The transgenic plasmid pBac-［3XP3-EGFP-SV40-AsCPR75-promoter-Cas9-SV40］ was constructed. In the G₁ generation, transgenic individuals exhibiting green fluorescence in the larval ocelli and adult compound eyes were identified. Inverse PCR analysis confirmed that the exogenous expression cassette was inserted at position 487 522 bp on scaffold25 in one transgenic line and at position 29 949 095 bp on scaffold14. Further analyses demonstrated that Cas9 transcripts were detectable in transgene-positive individuals, and the temporal expression trend of Cas9 was largely consistent with that of the endogenous AsCPR75.【Conclusion】 This study successfully established transgenic An. sinensis lines capable of stably expressing Cas9 in the integument. These lines provide valuable genetic tools for future research involving integument-conditional genome editing in An. sinensis, and for developing mosquito genetic control strategies targeting the integument.