基于线粒体COⅡ基因的中国大豆食心虫不同地理种群遗传分化分析

doi:10.16380/j.kcxb.2017.04.012

摘要/Abstract

摘要： 【目的】大豆食心虫 Leguminivora glycinivorella （Matsumura）是我国大豆Glycina max最重要的蛀荚害虫。本研究旨在探讨中国大豆食心虫不同地理种群的遗传分化情况。【方法】通过测定大豆食心虫19个地理种群208头老熟幼虫的线粒体COⅡ基因序列（723 bp），利用MEGA6.0和DnaSP 5.0等软件分析大豆食心虫不同地理种群的基因序列变异和遗传分化情况.【结果】在19个大豆食心虫地理种群中共获得208条COⅡ基因序列，发现61个变异位点，包含57个单倍型。总群体单倍型多样度Hd=0.8522，各地理种群单倍型多样度范围在0.1818~0.9546间，总群体的固定系数Fst=0.4619，总遗传分化系数Gst=0.1186，总基因流Nm=0.29。总群体Tajima’s D检验结果不显著，表明大豆食心虫在较近的历史时期未出现群体扩张现象，群体大小稳定。单倍型网络图、ML单倍型系统树及采用不同地理种群间遗传距离构建的UPGMA系统发育树均显示，贵阳（GY）和都安（DA)种群与其余地理种群分化明显。AMOVA分子变异分析结果显示，东北组、西北组、黄淮组、华南组及西南组种群组间所占总变异的比例大于组内。【结论】大豆食心虫不同地理种群间基因交流较少，整体的遗传多样性和遗传分化程度较高，遗传分化主要来自地理种群组间，且地理距离是影响不同地理种群间遗传距离的重要因素。

关键词: 大豆食心虫, 线粒体COⅡ基因, 地理种群, 遗传分化, 基因流

Abstract: 【Aim】 Leguminivora glycinivorella (Matsumura) is one of the major pests of soybean Glycina max in China. This study aims to investigate the genetic ifferentiation among different geographic populations of L. glycinivorella in mainland China. 【Methods】 A 723 bp segment of the mitochondrial cytochrome c oxidase subunit II (COII) gene in 208 mature larvae from 19 geographic populations of L. glycinivorella in China was sequenced and analyzed. The genetic differentiation and sequence variation were analyzed using MEGA6.0 and DnaSP 5.0.【Results】Among the alignment of 208 COII sequences from 19 geographic populations of L. glycinivorella， 61 variable sites and 57 haplotypes were identified. The total haplotype diversity index (Hd) was 0.8522， and the range of haplotype diversity of different populations was 0.1818-0.9546.The total fixed coefficient (Fst)， total genetic differentiation coefficient (Gst) and total gene flow (Nm) were 0.4619， 0.1186 and 0.29，respectively. No significant difference among different populations was found by Tajima’s D test, suggesting that there might be no population expansion in recent history of L. glycinivorella and the population size is stable. Analysis of haplotype networks， ML hylogenetic trees of haplotypes and UPGMA phylogenetic trees based on the genetic distance of different geographic populations showed that Guiyang (GY) and Du’an (DA) populations had obvious genetic differentiation with other populations. Molecular variance analysis (AMOVA) of geographic populations demonstrated that the percentage of variation among the population groups of Northeast China， Northwest China, Huang-Huai region, South China， and Southwest China was higher than those within each population group. 【Conclusion】The results revealed that there is less gene flow among different geographic populations of L. glycinivorella， which have high-degree whole genetic diversity and genetic differentiation. And the observed genetic differentiation rimarily occurs among population groups of different geographic regions. The geographic distance has important influence on the genetic distance of different geographic populations of L. glycinivorella.

Key words: Leguminivora glycinivorella, mitochondrial COII gene, geographic population, genetic differentiation, gene flow

朱诗禹, 徐伟, 高宇, 崔娟, 欧师琪, 史树森. 基于线粒体COⅡ基因的中国大豆食心虫不同地理种群遗传分化分析[J]. 昆虫学报, 2017, 60(4): 475-486.

ZHU Shi-Yu, Xu-Wei, Gao-Yu, Cui-Juan, OU Shi-Qi, Shi Shu-Sen. Analysis of the genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera： Olethreutidae) in China based on mitochondrial COII gene sequences[J]. Acta Entomologica Sinica, 2017, 60(4): 475-486.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.04.012

http://www.insect.org.cn/CN/Y2017/V60/I4/475

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基于线粒体COⅡ基因的中国大豆食心虫不同地理种群遗传分化分析

Analysis of the genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera： Olethreutidae) in China based on mitochondrial COII gene sequences

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