桑天牛卵啮小蜂雌蜂对茉莉酸诱导的桑枝挥发物的触角电位和嗅觉反应

doi:10.16380/j.kcxb.2017.09.005

›› 2017, Vol. 60 ›› Issue (9): 1013-1020.doi: 10.16380/j.kcxb.2017.09.005

桑天牛卵啮小蜂雌蜂对茉莉酸诱导的桑枝挥发物的触角电位和嗅觉反应

王薇¹, 李继泉^1,2,*, 崔建州^1,2, 李增良³，张情¹, 田红雨¹, 唐哲¹

（1. 河北农业大学林学院, 河北保定 071000; 2. 河北省林木种质资源和森林保护重点实验室, 河北保定 071000; 3. 河北省林果桑花质量监督检验管理中心, 石家庄 050081）

出版日期:2017-09-20 发布日期:2017-09-20

Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morus alba branches induced by jasmonic acid

WANG Wei¹, LI Ji-Quan^1,2,*, CUI Jian-Zhou¹,^2, LI Zeng-Liang³, ZHANG Qing¹, TIAN Hong-Yu¹, TANG Zhe¹

(1. College of Forestry, Agricultural University of Hebei, Baoding, Hebei 071000, China; 2. Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei Province, Baoding, Hebei 071000, China; 3. Center for Quality Supervision and Test of Forestry Fruit, Mullbery and Flower of Hebei Province, Shijiazhuang 050081, China)

Online:2017-09-20 Published:2017-09-20

摘要/Abstract

摘要： 【目的】明确桑天牛卵啮小蜂Aprostocetus prolixus雌蜂对茉莉酸诱导的桑Morus alba枝挥发物的触角电位和嗅觉反应，有利于揭示茉莉酸诱导桑枝产生间接抗性的化学机制。【方法】采用动态顶空气体法收集茉莉酸处理后的桑枝挥发物，分别通过气相色谱触角电位联用仪（GC-EAD）和气相色谱/质谱联用仪（GC/MS）筛选和鉴定对桑天牛卵啮小蜂雌蜂有EAD活性的化合物，并比较这些活性组分的释放速率在茉莉酸处理桑枝与蒸馏水处理的对照桑枝间的差异，最后利用Y-型嗅觉测定仪明确对该寄生蜂有引诱活性的组分。【结果】桑枝经茉莉酸处理后，其挥发物中的Z-3-己烯醇、γ-萜品烯、β-水芹烯、紫苏烯、水杨酸甲酯及3种未知化合物使桑天牛卵啮小蜂雌蜂产生明显的EAD反应，并且β-水芹烯、紫苏烯和水杨酸甲酯的释放量明显高于对照，其中β-水芹烯和紫苏烯仅存在于茉莉酸处理后桑枝的挥发物中，而Z-3-己烯醇的释放速率与对照无显著差异。0.1% β-水芹烯、0.1%紫苏烯以及0.01%和0.001%水杨酸甲酯对该蜂雌蜂具有显著的引诱作用。【结论】结果提示，茉莉酸促进桑枝大量释放β-水芹烯、紫苏烯和水杨酸甲酯，是茉莉酸诱导桑树对桑天牛产生间接抗性的重要机制。

关键词: 桑天牛卵啮小蜂, 桑枝, 外源茉莉酸, 挥发物, 触角电位反应, 嗅觉反应

Abstract: 【Aim】 This study aims to clarify the electroantennographic and olfactory responses of female wasps of the eulophid Aprostocetus prolixus to volatiles of Morus alba branches induced by jasmonic acid (JA), so as to reveal the chemical mechanisms of indirect resistance in M. alba branches against Apriona germari. 【Methods】 The volatiles from M. alba branches treated with JA were collected by using the dynamic headspace volatile collection method. The electroantennographically active compounds for female wasps of A. prolixus were screened and indentified by coupled gas chromatography-electroantennographic detector (GC-EAD) and GC/MS, respectively, and the differences in emission rates of the EAD-active components between the JAtreated M. alba branches and the water-treated control branches were also compared. Furthermore, the volatile compounds attractive to female A. prolixus were determined by Y-tube olfactometer. 【Results】 The results demonstrated that Z-3-hexenol, β-terpinene, β-phallandrene, perillene, methyl salicylate and other three unknown chemicals from M. alba branches treated with JA elicited significant GC-EAD responses in female wasps of A. prolixus. The emission rates of β-phallandrene, perillene and methyl salicylate from the JA-treated M. alba branches were significantly higher than those from the control branches. Furthermore, β-phallandrene and perillene were detected only in the volatiles of the JA-treated plants. However, there was no significant difference in the emission rate of Z-3-hexenol between the JA-treated plants and the control plants. And 0.1% β-phallandrene, 0.1% perillene and 0.01% and 0.001% methyl salicylate were significantly attractive to female wasps of A. prolixus. 【Conclusion】 The results suggest that highly elevated emission of β-phallandrene, perillene and methyl salicylate from the JA-treated M. alba branches is one of the important mechanisms of the JA-induced indirect resistance in M. alba tree against A. germari.

Key words: Aprostocetus prolixus, Morus alba, exogenous jasmomic acid, volatiles, electroantennographic response, olfactory response

王薇, 李继泉, 崔建州, 李增良, 张情, 田红雨, 唐哲. 桑天牛卵啮小蜂雌蜂对茉莉酸诱导的桑枝挥发物的触角电位和嗅觉反应[J]. , 2017, 60(9): 1013-1020.

WANG Wei, LI Ji-Quan, CUI Jian-Zhou, LI Zeng-Liang, Zhang-Qing, TIAN Hong-Yu, TANG Zhe. Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morus alba branches induced by jasmonic acid [J]. , 2017, 60(9): 1013-1020.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.09.005

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桑天牛卵啮小蜂雌蜂对茉莉酸诱导的桑枝挥发物的触角电位和嗅觉反应

Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morus alba branches induced by jasmonic acid

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