基于mtDNA COI基因的甘肃截形叶螨不同地理种群遗传分化分析

doi:10.16380/j.kcxb.2017.09.012

摘要/Abstract

摘要： 【目的】探讨甘肃截形叶螨Tetranychus truncatus种群遗传多样性、遗传分化及种群系统发育关系。【方法】本研究以2016年6-9月采自甘肃省8个不同生境的34个截形叶螨种群的518头螨样品进行mtDNA COI基因PCR扩增、测序，并利用MEGA7.0和Dna SP5.0等软件分析截形叶螨不同种群的mtDNA COI基因序列变异和遗传分化情况。【结果】在34个截形叶螨地理种群中共获得 518条mtDNA COI基因片段，测定片段长度为402 bp， T, C, A和G碱基平均含量分别为43.2%, 10.5%, 32.2%和14.0%，A+T含量（75.4%）明显高于C+G含量（24.6%），有明显的A/T偏倚性；核酸多样性性指数为0.0328。共检出12个单倍型，单倍型指数为0.8004，其中，H2出现了14次，分布于5个生态区域，为主体单倍型，占个体样本的41%。单倍型网络中介图表明各单倍型明显聚为5个聚类簇；12个单倍型的NJ系统发育树明显地分为4支，该结果与单倍型中介网络图的结果基本一致。各种群间的遗传距离在0.001~0.08955之间。UPGMA聚类图结果表明JQ（酒泉）种群和HS（合水）种群与其他种群相比分化明显。Mantel检测结果表明，种群间的遗传距离与地理距离无显著的相关性（r=0.043， P>0.05）。34个种群间的遗传分化指数Fst为0.11995，种群间变异比例为1.20％。【结论】甘肃省8个生态区的34个截形叶螨种群间的遗传距离与地理距离无显著相关性；截形叶螨的遗传变异主要来自于种群内部，种群间还未发生明显的遗传分化现象。

关键词: 截形叶螨, mtDNA COI, 种群遗传, 地理种群, 遗传分化, 甘肃

Abstract: 【Aim】 This study aims to explore the genetic diversity, genetic differentiation and phylogenesis of Tetranychus truncatus populations in Gansu province, northwestern China. 【Methods】 The mtDNA COI gene in 518 individual samples of T.truncatus, which were collected from 34 populations in 8 different habitats of Gansu province from June to September in 2016, was amplified, sequenced and analyzed. And its sequence variation and genetic differentiation were analyzed using MEGA 7.0, Dna SP 5.0 and other software. 【Results】 The sequenced fragment is 402 bp in length. The average contents of A, T, C and G are 32.2%, 43.2%, 10.5%, 14.0%, respectively. The A+T content (75.4%) was apparently higher than the G+C content (24.6%), with an obvious A/T bias, and the nucleic acid diversity was 0.0328. There were 12 haplotypes in 34 populations, and the haploid type index was 08004. In 12 haplotypes, H2 occurred in the highest number of individuals, which was distributed in 5 ecoregions, appeared 14 times, and occupied 41% in the number of individuals. Five cluster groups were clustered together in the haplotype mediation network diagram. NJ phylogenetic tree showed that 12 haplotypes were divided into four branches, and this result was the same as that of the mediation network diagram. Genetic distance of all the populations was among 0.001-0.08955. UPGMA dendrogram showed that JQ (Jiuquan) population and HS (Heshui) population had higber genetic differentiation with other populations. Mantel test result showed no significant correlation between genetic distance and geographical distance (r=0.043, P>0.05). The genetic differentiation index (Fst) among 34 populations was 0.11995, and the variation proportion among populations was 1.20%. 【Conclusion】 There is no significant correlation between genetic distance and geographical distance among 34 populations of T. truncatus in eight ecoregions of Gansu province. The genetic variation of T. truncatus is mainly within populations, and there is no obvious genetic differentiation among populations.

Key words: Tetranychus truncatus, mtDNA COI, population genetic, geographical population, genetic differentiation, Gansu

杨顺义, 周兴隆, 宋丽雯, 严晓彤, 张小云, 张新虎, 沈慧敏. 基于mtDNA COI基因的甘肃截形叶螨不同地理种群遗传分化分析[J]. , 2017, 60(9): 1083-1092.

YANG Shun-Yi, ZHOU Xing-Long, SONG Li-Wen, YAN Xiao-Tong, ZHANG Xiao-Yun, ZHANG Xin-Hu, SHEN Hui-Min. Analysis of genetic differentiation among different geographic populations of Tetranychus truncatus (Acari: Tetranychidae) in Gansu province, northwestern China based on mtDNA COI gene [J]. , 2017, 60(9): 1083-1092.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.09.012

http://www.insect.org.cn/CN/Y2017/V60/I9/1083

[1]	呼晓庆, 杨兆富. 基于线粒体COI、Cytb和COII基因的中国草地螟不同地理种群遗传分化分析[J]. 昆虫学报, 2019, 62(6): 720-733.
[2]	王运良, 潘忠琪, 陈可可, 陶瑞松, 苏成勇, 郝家胜, 杨群. 基于线粒体基因组控制区序列的中国冰清绢蝶遗传分化和谱系生物地理研究[J]. 昆虫学报, 2019, 62(4): 475-488.
[3]	谢艳兰, 张宏瑞, 李正跃. 基于线粒体COI基因的中国西南地区木领针蓟马地理种群的遗传分化分析[J]. 昆虫学报, 2019, 62(3): 370-380.
[4]	王克勤, 刘兴龙, 邵天玉. 中国东北地区亚洲玉米螟遗传多样性及寄主专化性分析[J]. , 2018, 61(9): 1054-1066.
[5]	李梅梅, 李伯辽, 仵均祥, 许向利. 我国粘虫种群的微卫星位点筛选及遗传多样性分析[J]. , 2018, 61(6): 712-720.
[6]	李菁, 汪洋洲, 张小飞, 刘津, 白明皓, 王振营. 新疆地区亚洲玉米螟中Wolbachia共生菌的检测和鉴定[J]. , 2018, 61(5): 555-564.
[7]	张亚楠, 牛黎明, 周世豪, 龚治, 李磊, 张方平, 韩冬银, 符悦冠. 基于微卫星分子标记的中国瓜实蝇不同地理种群遗传分化分析[J]. , 2018, 61(5): 613-619.
[8]	朱诗禹, 徐伟, 高宇, 崔娟, 欧师琪, 史树森. 基于线粒体COⅡ基因的中国大豆食心虫不同地理种群遗传分化分析[J]. 昆虫学报, 2017, 60(4): 475-486.
[9]	魏书军, 李冰艳, 曹利军, 朱家颖. RAD-seq技术及其在昆虫学研究中的应用[J]. , 2016, 59(7): 767-774.
[10]	郭慧萍, 周姝婧, 朱翔杰, 徐新建, 于瀛龙, 杨凯杰, 陈道印, 周冰峰. 秦巴山区中华蜜蜂种群微卫星DNA遗传分析[J]. , 2016, 59(3): 337-345.
[11]	谷瑛, 宾先丽, 刘璐, 周俊, 马振刚, 谭宏伟, 周泽扬, 许金山. 重庆中华蜜蜂的形态和遗传多样性[J]. , 2016, 59(12): 1386-1394.
[12]	张鹏飞, 周晓榕, 庞保平, 常静, 单艳敏, 张卓然. 蒙古沙葱萤叶甲种群遗传多样性的微卫星分析[J]. , 2015, 58(9): 1005-1011.
[13]	娄恒, 陈雅婷, 张艳凯, 杨现明, 孙荆涛, 洪晓月. Wolbachia 在花蓟马中的感染及其与寄主线粒体DNA多样性的关系[J]. , 2015, 58(7): 750-760.
[14]	黄振, 陈韶萍, 谢婧, 郭琼霞. 应用种特异性PCR技术快速鉴定辣椒实蝇[J]. , 2015, 58(4): 460-466.
[15]	Arvind SHARMA, Richa SHARMA, Ashwani KUMAR, Madhulika DUBE, Surendra Kumar GAKHA. 基于微卫星标记的印度中部 Madhya Pradesh 地区疟疾强化控制和非强化控制区的斯氏按蚊基因流和种群遗传结构分析（英文）[J]. , 2015, 58(1): 74-81.

基于mtDNA COI基因的甘肃截形叶螨不同地理种群遗传分化分析

Analysis of genetic differentiation among different geographic populations of Tetranychus truncatus (Acari: Tetranychidae) in Gansu province, northwestern China based on mtDNA COI gene

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10