›› 2017, Vol. 60 ›› Issue (10): 1120-1128.doi: 10.16380/j.kcxb.2017.10.003

• 研究论文 • 上一篇    下一篇


宋月芹, 董钧锋, 陈庆霄, 胡振杰, 孙会忠*   

  1. (河南科技大学林学院, 河南洛阳 471000)
  • 出版日期:2017-10-20 发布日期:2017-10-20

Analysis of the antennal transcriptome and chemoreception-related genes of the bean bug, Riptortus pedestris (Hemiptera: Alydidae)

SONG Yue-Qin, DONG Jun-Feng, CHEN Qing-Xiao, HU Zhen-Jie, SUN Hui-Zhong*   

  1.  (Forestry College, Henan University of Science and Technology, Luoyang, Henan 471000, China)
  • Online:2017-10-20 Published:2017-10-20

摘要: 【目的】点蜂缘蝽Riptortus pedestris主要为害豆科作物,成虫和若虫刺吸汁液,造成严重损失,目前对该虫的研究较少,基因信息缺乏。本研究旨在获得点蜂缘蝽的触角转录组数据,开发基于嗅觉防治害虫的新方法。【方法】利用Illumina HiSeqTM 4000高通量测序技术测定了点蜂缘蝽成虫触角转录组,并进行了生物学信息分析。【结果】共获得45 802 812条clean reads,包括6.87 Gb(GenBank登录号: SRR4429103)。拼接92 259条unigenes,平均长度为618 bp,N50为1 013 bp。在7大数据库中注释到21 365条unigenes。通过进一步转录组数据分析,我们鉴定出219个点蜂缘蝽化学感受相关基因,包括188个嗅觉受体、6个味觉受体、2个离子型受体、4个感觉神经元膜蛋白、8个气味结合蛋白和11个化学感受蛋白。氨基酸序列分析发现,RpedOBP1和RpedOBP2在第6个保守的半胱氨酸后又多了3个保守的半胱氨酸位点,属于加Plus-C气味结合蛋白家族。【结论】本研究获得了点蜂缘蝽触角转录组数据,并鉴定出嗅觉相关基因,结果为今后利用嗅觉基因靶标防治点蜂缘蝽提供了重要的分子生物学信息数据。

关键词: 点蜂缘蝽, 触角转录组, 高通量测序, 基因注释, 化学感受相关基因

Abstract: 【Aim】 Riptortus pedestris is a major soybean pest which feeds juice in the stages of adult and nymph, causing serious damage to production. However, R. pedestris has been rarely researched, that leads to the lack of genomic resources. The objective of this study is to obtain the antennal transcriptome data of R. pedestris and to seek new methods for pest control through olfaction. 【Methods】 The adult antennal transcriptome of R. pedestris is sequenced using Illumina HiSeqTM 4000 platform and analyzed bioinformatically. 【Results】 In total, 45 802 812 clean reads with 6.87 Gb were obtained (GenBank accession no.: SRR4429103). There were 92 259 unigenes with the mean length of 618 bp and an N50 of 1 013 bp. A total of 21 365 unigenes were annotated based on seven databases. By further analyzing the transcriptome data, we identified 219 chemoreception-related genes including 188 for olfactory receptors (ORs), 6 for gustatory receptors (GRs), 2 for ionotropic receptors (IRs), 4 for sensory neuron membrane proteins (SNMPs), 8 for odorant-binding proteins (OBPs) and 11 for chemosensory proteins (CSPs). The analysis of amino acid sequence indicated that RpedOBP1 and RpedOBP2 have three additional conserved cysteine residues immediately after the sixth cysteine and belong to the plus-C OBP family. 【Conclusion】 This study acquired the antennal transcriptom data of R. pedestris and identified olfaction-related genes. The results provide the important information data of molecular biology for the control of R. pedestris using olfaction-related gene targets.

Key words: Riptortus pedestris, antennal transcriptome, high-throughput sequencing, gene annotation, chemoreception-related genes