关键词: 绿豆象, 触角转录组, 高通量测序, 基因注释, 嗅觉相关基因

Abstract: 【Aim】 This study aims to establish the antennal transcriptome database of the azuki bean weevil, Callosobruchus chinensis, and to obtain the genetic data. 【Methods】The antennal transcriptome of C. chinensis adults was sequenced using an Illumina HiSeq 4000 platform and subjected to bioinformatics analysis. 【Results】 A total of 51.10 Gb clean data (NCBI SRA accession no.: SRP119884) were obtained, and the clean reads were assembled into 83 535 unigenes with an N50 length of 1 492 bp and 15 075 unigenes over 1 kb in length. A total of 22 148 unigenes in the antennal transcriptome of C. chinensis adults were annotated using BLASTX search against the public databases. Most unigenes (18 744 unigenes) were annotated to NR database, and the unigenes of C. chinensis had the highest similarity (39.57%) with those of Tribolium castaneum. All unigenes were divided into 54 branches of three categories (cellular components, molecular function and biological processes) using Gene Ontology (GO), and a lot of unigenes were annotated to be related to metabolic processes, binding activities and cellular processes. In KEGG database, 9 084 unigenes were assigned to 289 known metabolic pathways. Among these, most unigenes (516) are involved in metabolic pathways of ribosome. By further screening and identification, 140 olfaction-related genes of C. chinensis including 12 odorant binding protein (OBP) genes, 4 chemosensory protein (CSP) genes, 116 odorant receptor (OR) genes, 1 ionotropic receptor (IR) gene and 7 sensory neuron membrane protein (SNMP) genes were identified, and their expression levels were evaluated based on FPKM value. 【Conclusion】 This study obtained the antennal transcriptome data of C. chinensis. The results provide a foundation for the further study of gene functions and molecular mechanisms of olfactory reception of C. chinensis.

Key words: Callosobruchus chinensis, antennal transcriptome, HiSeq, gene annotation, olfaction-related genes