关键词: 小黑杨, 基因转化, 杀虫毒蛋白基因, 害虫防治, 舞毒蛾, 蜘蛛毒蛋白

Abstract: In recent years, the pest insects on hybrid poplar Populus simonii×P. nigra broke out heavily, which caused great losses in forestry. In order to improve insect resistance of P. simonii×P. nigra and avoid pollution due to insecticides, the fused BGT gene consisting of the insecticidal toxin gene from the spider, Atrax robustus, and the C terminal of CryⅠA（b）gene from Bacillus thuringiensis was transferred into P. simonii×P. nigra by Agrobacteriumm-mediated transformation system. The results of  PCR and Southern blotting analyses showed that the insecticidal gene of BGT was integrated into the genome of  P. simonii×P. nigra. The corrected mortality of the second instar of Lymantria dispar in 6 days and 9 days after they were fed with the transgenic poplars was 37.0% and 92.6%, respectively. Analysis of variance showed that there was significant difference in body weight between L. dispar larvae fed with the transformed poplars and those fed with untransformed poplars. The results indicated that the growth rate of L. dispar fed with the transgenic poplars was affected negatively.

Key words: Hybrid poplar, gene transformation, insecticidal neurotoxin gene, insect control, Lymantria dispar, spider neurotoxin