关键词: 棉铃虫, Gqα, 触角cDNA文库, 酵母双杂交技术, 信号转导, 信息素结合蛋白

Abstract:

G proteins are important signalling components. Many findings indicated that members of Gq family were functionally diverse than previously thought. In order to determine the exact functions of the α subunit of Gq family of G proteins (Gqα) in signal transduction pathway, Gqα was used as the bait to screen the antennacDNA library of the cotton bollworm Helicoverpa armigera by the two-hybrid system. More than ten proteins including pheromone binding protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein S20, cytochrome oxidase subunit Ⅰ, allatotropin neuropeptide precursor (AT2), translationally controlled tumor protein (TCTP), humoral lectin prepropeptide and glycosylasparaginase were found to interact with Gqα, suggesting that Gqα plays diverse roles in signal transduction. Among the diverse functions, Gqα is most likely to be involved in the pheromone detection among olfactory signalling. The findings may provide a primary basis for future construction of an integrated signalling network in the moth.

Key words: Helicoverpa armigera, Gqα, antenna cDNA library, yeast two-hybrid technique, signal transduction, pheromone binding protein