›› 2010, Vol. 53 ›› Issue (12): 1339-1344.

• 研究论文 • 上一篇    下一篇


苏宏华, 王桂荣, 张永军, 梁革梅, 吴孔明, 郭予元   

  • 出版日期:2011-01-29 发布日期:2010-12-20
  • 通讯作者: 郭予元

Screening of proteins interacting with Gqα by yeast two-hybrid technique in the antenna of Helicoverpa armigera (Lepidoptera: Noctuidae)(In English)

SU Hong-Hua, WANG Gui-Rong, ZHANG Yong-Jun, LIANG Ge-Mei, WU Kong-Ming, GUO Yu-Yuan   

  • Online:2011-01-29 Published:2010-12-20
  • Contact: GUO Yu-Yuan


G蛋白在信号转导中起着重要的作用, 许多研究表明Gq蛋白还具有其他功能。为了明确Gqα 在信号转导中的具体作用, 本研究以Gqα为诱饵, 利用酵母双杂交技术对棉铃虫Helicoverpa armigera Hübner)触角cDNA文库进行筛选。结果表明: 信息素结合蛋白(PBP)、 3-磷酸甘油醛脱氢酶 (GAPDH) 核糖体蛋白S20、细胞色素氧化酶亚基 促咽侧体神经肽前体 (AT2) 翻译控制的肿瘤蛋白 (TCTP)、体液凝集素前多肽原和糖基化天冬酰胺酶等10多种蛋白与Gqα发生结合。据此推断: Gqα可能在信号转导过程中发挥多种作用; 在多种功能中, Gqα最可能参与嗅觉信号传导中的信息素识别。这些研究结果可能为将来建立棉铃虫完整的信号网络提供基础。

关键词: 棉铃虫, Gqα, 触角cDNA文库, 酵母双杂交技术, 信号转导, 信息素结合蛋白


G proteins are important signalling components. Many findings indicated that members of Gq family were functionally diverse than previously thought. In order to determine the exact functions of the α subunit of Gq family of G proteins (Gqα) in signal transduction pathway, Gqα was used as the bait to screen the antennacDNA library of the cotton bollworm Helicoverpa armigera by the two-hybrid system. More than ten proteins including pheromone binding protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein S20, cytochrome oxidase subunit , allatotropin neuropeptide precursor (AT2), translationally controlled tumor protein (TCTP), humoral lectin prepropeptide and glycosylasparaginase were found to interact with Gqα, suggesting that Gqα plays diverse roles in signal transduction. Among the diverse functions, Gqα is most likely to be involved in the pheromone detection among olfactory signalling. The findings may provide a primary basis for future construction of an integrated signalling network in the moth.

Key words: Helicoverpa armigera, Gqα, antenna cDNA library, yeast two-hybrid technique, signal transduction, pheromone binding protein