›› 2010, Vol. 53 ›› Issue (12): 1360-1366.

• 研究论文 • 上一篇    下一篇

Cry1Ac毒蛋白对粘虫生长发育、繁殖及飞行能力的影响

蒋善军, 罗礼智, 胡毅, 张蕾   

  • 出版日期:2011-01-17 发布日期:2010-12-20
  • 通讯作者: 罗礼智

Effects of Cry1Ac protein on growth and development, reproduction and flight potential of the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae)

JIANG Shan-Jun, LUO Li-Zhi, HU Yi, ZHANG Lei   

  • Online:2011-01-17 Published:2010-12-20
  • Contact: LUO Li-Zhi

摘要:

为了更全面地评价转基因作物对粘虫Mythimna separata (Walker)(鳞翅目: 夜蛾科)的影响, 在室内用生测法研究了粘虫初孵幼虫连续取食含不同浓度的Cry1Ac毒蛋白的人工饲料后, 其生长发育、繁殖及飞行能力的变化。结果表明: 取食0(对照), 3, 6, 12, 24, 4896 μg/g Cry1Ac毒蛋白人工饲料6 d后的幼虫头宽、 体长、 18 d后的体重随着毒蛋白浓度的升高显著降低, 幼虫发育历期显著延长, 死亡率显著升高。取食含毒蛋白浓度>12 μg/g饲料的幼虫不能正常化蛹; 取食含0, 36 μg/g毒蛋白饲料的幼虫能正常化蛹、 羽化, 化蛹率随着毒蛋白浓度的升高而显著降低、 蛹历期显著延长; 取食含0, 36 μg/g毒蛋白饲料的幼虫羽化的成虫繁殖能力有显著差异, 产卵前期随毒蛋白浓度的升高显著延长, 产卵量显著降低; 1日龄成虫飞行能力(飞行距离、 时间和速度)则随毒蛋白浓度的升高显著降低。这些结果表明, Cry1Ac毒蛋白对粘虫的生长发育、 繁殖及飞行具有显著影响, 从而为完善转Bt作物的生态安全性风险评估提供了重要的实验依据。

关键词: Cry1Ac毒蛋白, 粘虫, 生长, 发育, 繁殖, 飞行能力

Abstract:

To obtain a fullscale assessment of the influences of transgenic Bt crops on the oriental armyworm, Mythimna separata (Walker), larval growth and development, adult reproduction, and flight potential of M. separata were examined when newly hatched larvae continuously fed on artificial diet containing different concentrations of Cry1Ac toxin. The results showed that the mean head capsule width, and body length of the larvae fed with diets containing 0 (CK), 3, 6, 12, 24, 48 and 96 μg/g of Cry1Ac toxin for 6 d were significantly decreased as the concentration of toxin increased. Similarly, body weight, mortality, and the developmental period of the larvae treated for 18 d were negatively and significantly affected as the Cry1Ac dosage increased. The larvae fed with diets containing more than 12 μg/g of Cry1Ac toxin could not pupate, while the larvae fed with diets containing 0, 3 and 6 μg/g of Cry1Ac toxin could pupate and emerge normally. Furthermore, the pupal duration was extended, and the pupation rate decreased significantly as Cry1Ac concentration increased from 0 (CK) to 3, and 6 μg/g. Reproductive potential of the moths resulting from the larvae fed with diets containing 0, 3, and 6 μg/g of Cry1Ac toxin was also significantly different. Preoviposition period of adults was significantly prolonged, and the number of eggs laid per female was significantly decreased as the dosage of Cry1Ac toxin increased. Flight potential (including flight duration, distance, and velocity) of the 1-day-old moths was significantly decreased as the dosage of Cry1Ac toxin increased by tethered-flight test. All these results showed that the Cry1Ac had a significant impact on growth and development, reproduction and flight potential of M. separata, and this will help to improve the evaluation of ecological safety of Bt-crops.

Key words: Cry1Ac toxin, Mythimna separata, growth, development, reproduction, flight potential