溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响

摘要/Abstract

摘要： 【目的】探讨溴氰虫酰胺对甜菜夜蛾 Spodoptera exigua (Hübner)的亚致死效应，为甜菜夜蛾的综合防治和溴氰虫酰胺的合理使用提供理论依据。【方法】采用饲料混毒法，测定溴氰虫酰胺对甜菜夜蛾3龄幼虫的毒力，确定其亚致死剂量（LC₁₀和LC₂₅），并分别用该亚致死剂量处理甜菜夜蛾3龄幼虫，研究其对甜菜夜蛾的生长发育和体内3种解毒酶活性的影响。【结果】以LC₁₀ 和LC₂₅剂量处理甜菜夜蛾3龄幼虫后，幼虫生长受到显著抑制，虫重抑制率分别为11.55%和27.68%；LC₁₀和LC₂₅处理组3龄幼虫到化蛹天数分别比对照组延长0.07和0.20 d；化蛹率显著低于对照组（80.93%），分别为63.89%和49.43%；成虫寿命显著缩短1.11 d和2.08 d；单雌产卵量和卵孵化率也低于对照组。亚致死剂量溴氰虫酰胺处理甜菜夜蛾幼虫后24-96 h，羧酸酯酶活性和谷胱甘肽-S转移酶活性表现出相似的变化趋势，呈先激活升高、后被抑制降低；而多功能氧化酶活性在药剂处理48 h和72 h均受到不同程度的抑制作用，这种抑制作用与浓度成正比。【结论】亚致死剂量的溴氰虫酰胺对甜菜夜蛾的生长发育有显著抑制作用，对其幼虫体内解毒酶活性也有不同程度的抑制作用。

关键词: 甜菜夜蛾, 溴氰虫酰胺, 亚致死效应, 生长发育, 解毒酶

Abstract: 【Aim】 This study aims to explore the sublethal effects of cyantraniliprole on Spodoptera exigua (Hübner)so as to provide a scientific basis for the integrated management program for S. exigua and the rational use of cyantraniliprole. 【Methods】 The toxicity of cyantraniliprole on the 3rd instar larvae of S. exigua was assayed with diet-incorporation bioassays, and the effects of cyantraniliprole at two sublethal doses (LC₁₀and LC₂₅) on the growth and development of S. exigua and the activities of three detoxifying enzymes were assayed. 【Results】 When the S. exigua larvae were treated with two sublethal doses of cyantraniliprole (LC₁₀and LC₂₅), their growth was significantly restrained compared to that in the control group, the average body weight was inhibited by 11.55% and 27.68% and the developmental durations from the 3rd instar larva to pupa were elongated by 0.07 and 0.20 d, respectively. The pupation rates in the LC₁₀and LC₂₅treatment groups were 63.89% and 49.43% and were significantly lower than that of the control (80.93%). The adult longevity in the LC₁₀and LC₂₅ treatment groups was obviously shortened by 1.11 d and 2.08 d, respectively, and the number of eggs laid per female and the egg hatching rates in the two treatment groups were significantly lower than those of the control. The activities of carboxylesterase (CarE) and glutathione- S-transferases (GSTs) in the 3rd instar larvae treated with the two sublethal doses of cyantraniliprole were promoted first and then decreased from 24 h to 96 h aftertreatment. The activities of mixed function oxidase (MFO) were also inhibited at 48 h and 72 h after treatment, and the inhibition effect was in proportion to the cyantraniliprole dose. 【Conclusion】 These results demonstrate that sublethal dose of cyantraniliprole can significantly restrain the growth and development of S. exigua and the activities of three detoxifying enzymes in its larvae.

Key words: Spodoptera exigua, cyantraniliprole, sublethal effect, growth and development, detoxifying enzyme

余慧灵, 向兴, 袁贵鑫, 陈羿渠, 王学贵. 溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J]. , 2015, 58(6): 634-641.

YU Hui-Ling, XIANG Xin, YUAN Gui-Xin, CHEN Yi-Qu, WANG Xue-Gui. Effects of sublethal doses of cyantraniliprole on the growth and development and the activities of detoxifying enzymes in Spodoptera exigua (Lepidoptera: Noctuidae)[J]. , 2015, 58(6): 634-641.

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溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响

Effects of sublethal doses of cyantraniliprole on the growth and development and the activities of detoxifying enzymes in Spodoptera exigua (Lepidoptera: Noctuidae)

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