昆虫学报 ›› 2017, Vol. 60 ›› Issue (3): 247-253.doi: 10.16380/j.kcxb.2017.03.002

• 研究论文 • 上一篇    下一篇

家蚕末龄幼虫、蛹和成虫期载脂蛋白组成的变化 (英文)

Thangaraj PRATHEEP1, Neelamegam RAMESHKUMAR1,*, Nagarajan KAYALVIZHI2, Thangaiyan SUGANYA1, Sivashanmugam KARTHIKEYAN3, Muthukalingan KRISHNAN1   

  1. (1. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli-620024, India; 2. Department of Zoology, Periyar University, Salem-636011, India; 3. School of Bio Sciences & Technology, VIT University, Vellore-632014, India)
  • 出版日期:2017-03-20 发布日期:2017-03-20

Changes in lipophorin composition during late larval, pupal and adult stages of Bombyx mori (In English)#br#

Thangaraj PRATHEEP1, Neelamegam RAMESHKUMAR1,*, Nagarajan KAYALVIZHI2, Thangaiyan SUGANYA1, Sivashanmugam KARTHIKEYAN3, Muthukalingan KRISHNAN1   

  1. (1. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli-620024, India; 2. Department of Zoology, Periyar University, Salem-636011, India; 3. School of Bio Sciences & Technology, VIT University, Vellore-632014, India)
  • Online:2017-03-20 Published:2017-03-20

摘要: 【目的】载脂蛋白是一种重要的血淋巴脂蛋白,它将90%的脂类输送到内部器官。本研究旨在调查家蚕Bombyx mori各个发育期血淋巴载脂蛋白组成变化。【方法】采用溴化钾密度梯度超离心、肝素钠沉淀(heparin sodium precipitation)和连接蒸发光散射检测器的高效液相色谱 (high performance liquid chromatography coupled with evaporative light scattering detector, HPLC-ELSD)等方法,检测了家蚕各个发育期血淋巴脂蛋白组成变化。【结果】从5龄幼虫到成虫的转变过程中,从幼虫期和吐丝期家蚕中分离的载脂蛋白密度分别为1.130 和1.139 g/mL,但蛹期载脂蛋白密度降低(为1.083 g/mL)。应用肝素钠沉淀法对载脂蛋白组成的分析表明,家蚕吐丝期载脂蛋白主要是高密度载脂蛋白(9.8 μg/μL) ;从吐丝期开始,低密度载脂蛋白含量增加,并在蛹期持续存在(4.2 μg/μL)。在所有发育期,载脂蛋白Ⅲ含量低,在2日龄蛹中含量最高(4.0 μg/μL)。另外,从吐丝期至成虫期,总脂浓度逐渐增加,从1.4 μg/μL增加至3.8 μg/μL。通过HPLC-ELSD对中性脂组成的分析表明,在所有发育期只含有微量的单酰甘油(<0.04 μg/μL),在5龄幼虫中期至蛹期末观察到二酰甘油(0.11~0.61 μg/μL),在蛹期发现三酰甘油(0.18~1.02 μg/μL)。【结论】总体结果说明,在幼虫期和吐丝期,高密度载脂蛋白主要运输二酰甘油;化蛹后,高密度载脂蛋白更多地与三酰甘油有关,而与二酰甘油相关性小,并与载脂蛋白Ⅲ形成低密度载脂蛋白有关。这些结果提示载脂蛋白的转变是一个精确的时间控制过程。

关键词: 家蚕, 载脂蛋白, 高密度载脂蛋白, 低密度载脂蛋白, 脂类

Abstract: 【Aim】 Lipophorin is a vital hemolymph lipoprotein, which delivers 90% of lipids to internal organs. This study aims to investigate the lipophorin composition during late larval, pupal and adult stages of the silkworm, Bombyx mori. 【Methods】 The compositional changes of hemolymph lipoprotein during various metamorphic stages were tested using potassium bromide density gradient ultracentrifugation, heparin sodium precipitation, and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) methods. 【Results】 During the transition from the 5th instar larva to adult, the isolated lipophorin density was 1.130 g/mL in larval stage and 1.139 g/mL in spinning stage, but decreased in pupal stage (1.083 g/mL). The lipophorin subspecies estimated using a heparin sodium precipitation method showed that high density lipophorin was predominant (9.8 μg/μL) in the spinning stage. Low density lipophorin was increased from spinning stage and constantly present in pupal stage (4.2 μg/μL). Meantime the apolipophorin III content was low in all metamorphic stages and reached the highest in 2-day-old pupa (4 μg/μL). On the other hand, total lipid concentration gradually increased from the spinning stage to pupal stage (from 0.5 to 3.8 μg/μL). Neutral lipid composition estimated by HPLC-ELSD showed that monoacylglycerol was trace amount in all stages (<0.04 μg/μL). Diacylglycerol was noted in mid-5th instar larval to end of pupal stage (0.11-0.61 μg/μL), and triacylglycerol was found in pupal stage (0.18-1.02 μg/μL). 【Conclusion】 Overall results indicated that high density lipophorin mainly transported diacylglycerol during larval and spinning stages. After pupation, high density lipophorin was associated with more triacylglycerol than diacylglycerol, and with apolipophorin III to form the low density lipophorin. These observations suggest that lipophorin conversions are precisely timed processes.

Key words:  Bombyx mori, lipophorin, high density lipophorin (HDLp), low density lipophorin (LDLp), lipid