四种重要医学媒介蚊虫离子受体基因IR8a和IR25a的特征及分类地位

doi:10.16380/j.kcxb.2017.04.003

摘要/Abstract

摘要： 【目的】鉴定中华按蚊Anopheles sinensis、冈比亚按蚊Anopheles gambiae、埃及伊蚊Aedes aegypti和致倦库蚊Culex quinquefasciatus 4种重要医学媒介蚊虫的离子受体基因IR8a与IR25a以及代表性的iGluRs基因和IRs基因，研究这些基因的特征及其系统发育关系，确定IR8a与IR25a基因在离子谷氨酸受体基因中的分类地位。【方法】以黑腹果蝇Drosophila melanogaster的iGluRs和IRs基因序列作为询问序列，用BLASTP和BLASTN方法分别搜索中华按蚊、冈比亚按蚊、埃及伊蚊和致倦库蚊的基因组数据库，以鉴定这4个种内的iGluRs和IRs基因序列；用生物信息学方法比较分析鉴定获得的IR8a与IR25a及代表性的iGluRs和IRs基因氨基酸序列的特征；用最大似然法、贝叶斯法和最大简约法构建并讨论这些基因氨基酸序列的系统发育关系；用 PAML软件计算这些基因的dN/dS(ω)值并分析其选择压力。【结果】通过对中华按蚊、冈比亚按蚊、埃及伊蚊和致倦库蚊中离子谷氨酸受体基因进行生物信息学鉴定，获得了所有IR8a和IR25a基因，24个代表性的iGluRs基因和30个代表性的IRs基因的序列。特征比较和系统发育关系分析结果表明，在4种蚊虫和黑腹果蝇中IR8a与IR25a基因的氨基酸序列长度与iGluRs的更接近，其平均值远大于其他IRs的；IR25a和IR8a有与传统iGluRs相同的氨基酸末端区域（amino-terminal domain， ATD），虽然IR8a的序列不太保守，而IRs则没有ATD；IR8a与IR25a序列有与传统iGluRs一样保守的配体结合区S1和S2，而IRs没有。所有这些基因的ω值远小于1，意味着这些基因在进化过程中都经历了纯化选择，而IR8a与IR25a的ω值更接近iGluRs。此外，IR8a与IR25a还有与non-NMDA相似的特异性位点，而这些位点在IRs不存在。再者，IR8a和IR25a与 non-NMDA聚在一枝，形成一个姐妹群，群内序列间的遗传距离小于本研究中任何其他序列间的距离。根据这些研究结果，我们将IR8a与IR25a分类进入传统iGluRs中的 non-NMDA类型，并命名为Putative受体。【讨论】本研究构建了蚊虫iGluRs和IRs基因的信息框架，确定了IR8a与IR25a基因的分类地位，对进一步的功能研究具有重要意义。

关键词: 蚊虫, iGluRs, IRs, IR8a, IR25a, 全基因组鉴定, 序列特征, 系统发育

Abstract: 【Aim】 To identify the ionotropic receptor genes IR8a and IR25a ， and the representative iGluRs and IRs genes in four vector mosquito species of medical importance， Anopheles sinensis， Anopheles gambiae， Aedes aegypti and Culex quinquefasciatus， and to investigate the characteristics and phylogenetic relationships of these genes， so as to determine the classification position of the IR8a and IR25a genes in the group of ionotropic glutamate receptor genes. 【Methods】 By using BLASTP and BLASTN searching against the genomes of these four mosquito species with the iGluRs and IRs gene sequences of Drosophila melanogaster as query sequences， the sequences of iGluRs and IRs genes of these four mosquito species were identified. The characteristics of the amino acid sequences of IR8a and IR25a and the representative iGluRs and IRs genes identified were predicted and comparatively analyzed by bioinformatics methods. The phylogenetic relationships of these genes in the five species were deduced and discussed by using maximum likelihood， Bayesian and maximum parsimony methods based on amino acid sequences， and the selection pressures of these genes were analyzed with the dN/dS(ω) values calculated by using PAML software. 【Results】 The sequences of IR8a and IR25a， 24 representative traditional iGluRs genes and 30 representative IRs genes in mosquitoes An. sinensis， An. gambiae， An. aegypti and Cx. quinquefasciatus were identified by bioinformatics methods. The comparative analyses of characteristics and phylogenetic relationships of these genes showed that the amino acid sequence lengths of IR8a and IR25a in the four mosquito species and D. melanogaster are closer to those of iGluRs， and their mean values are significantly longer than those of other IRs. IR25a has the aminoterminal domain (ATD) that is the characteristic of traditional iGluRs， and IR8a also has a region similar to ATD， although it is not conservative， while IRs have no ATD. IR8a and IR25 also have the conserved S1 and S2 ligand-binding domain (LBD)， the characteristics of iGluRs， but IRs do not have it. The dN/dS(ω) values of all these genes were far less than 1， suggesting the purifying selection in evolution， while the dN/dS(ω) values of IR8a and IR25a were closer to those of iGluRs. In addition， like non-NMDA， IR8a and IR25a have specific sites which do not exit in IRs. IR8a and IR25a cluster together with non-NMDA to form a sister group， with the genetic distance between the sequences within the group less than that of any other pairs of sequences in this study. Based on these results， IR8a and IR25a were classified into the non-NMDA receptors in iGluRs， and named as Putative receptors. 【Conclusion】 This study developed the information frame of iGluRs and IRs genes of mosquito species and determined the classification position of IR8a and IR25a genes， which is of significance for further functional study of these genes.

Key words: Culicidae, iGluRs, IRs, IR8a, IR25a, genome-wide identification, sequence characteristics, phylogeny

王婷婷, 郝友进, 何正波, 梅婷, 陈斌. 四种重要医学媒介蚊虫离子受体基因IR8a和IR25a的特征及分类地位[J]. 昆虫学报, 2017, 60(4): 379-399.

WANG Ting-Ting, Hao You-Jin, He Zheng-Bo, Mei-Ting, Chen-Bin. Characteristics and classification position of the ionotropic receptor genes IR8a and IR25a in four vector mosquito species of medical importance[J]. Acta Entomologica Sinica, 2017, 60(4): 379-399.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2017.04.003

http://www.insect.org.cn/CN/Y2017/V60/I4/379

[1]	苗颖, 花保祯. 淡黄蚊蝎蛉染色体特征及其系统发育意义[J]. 昆虫学报, 2019, 62(6): 734-742.
[2]	呼晓庆, 杨兆富. 基于线粒体COI、Cytb和COII基因的中国草地螟不同地理种群遗传分化分析[J]. 昆虫学报, 2019, 62(6): 720-733.
[3]	张方梅, 刘杨, 李祥瑞, 张云慧, 程登发, 郭文超, 吐尔逊·阿合买提. 马铃薯甲虫气味结合蛋白的序列和基因表达谱分析[J]. 昆虫学报, 2019, 62(4): 428-441.
[4]	黄卫东, 梁馨月, 谢秀凤, 王兴民, 陈晓胜. 基于12S, 16S和28S rRNA基因序列的中国小毛瓢虫属系统发育分析[J]. 昆虫学报, 2019, 62(2): 241-254.
[5]	李绍建, 高蒙, 王娜, 崔小伟, 桑素玲, 范腕腕, 王振宇. 河南不同地区棉蚜种群共生菌Wolbachia的检测及系统发育分析 [J]. 昆虫学报, 2019, 62(2): 215-221.
[6]	张锋, 洪波, 王远征, 李英梅, 陈志杰. 枣食芽象甲线粒体基因组全序列测定与系统发育分析[J]. 昆虫学报, 2019, 62(11): 1305-1314.
[7]	毛启萌, 李廷景, 付文博, 闫振天, 陈斌. 林氏按蚊线粒体全基因组序列的测定及基于线粒体基因组的按蚊属系统发育分析(英文)[J]. 昆虫学报, 2019, 62(1): 101-116.
[8]	刘贵芬, 房刚奇, 詹帅, 黄勇平. 亮斑扁角水虻脂肪酸去饱和酶基因的鉴定及其在双翅目中的进化格局[J]. , 2018, 61(8): 905-913.
[9]	李玲, 周渊涛, 谭瑶, 周晓榕, 庞保平. 沙葱萤叶甲化学感受蛋白基因的鉴定及表达谱分析(英文)[J]. , 2018, 61(6): 646-656.
[10]	李菁, 汪洋洲, 张小飞, 刘津, 白明皓, 王振营. 新疆地区亚洲玉米螟中Wolbachia共生菌的检测和鉴定[J]. , 2018, 61(5): 555-564.
[11]	林爱丽, 李欣欣, 赵新成, 宋南. 黑毛皮蠹线粒体基因组分析及皮蠹科系统发育分析[J]. , 2018, 61(4): 477-487.
[12]	马英, 李海龙, 何建, 赵延梅, 杨汉青, 鲁亮, 刘起勇. 基于线粒体COI基因的青海省44种蚤类的分子鉴定及系统发育分析[J]. , 2018, 61(4): 488-497.
[13]	任晓晓, 贺兴江, 龚雪阳, 赵文正, 刘意秋, 董坤 . 基于COⅠ基因序列的蜜蜂属系统发育分析及其舞蹈和筑巢行为进化分析[J]. 昆虫学报, 2018, 61(12): 1453-1461.
[14]	张贺贺, 张琪文, 林嘉, 季清娥, 陈家骅. 南亚实蝇鞣化激素基因的克隆与表达分析[J]. , 2018, 61(10): 1237-1246.
[15]	尹华春, 张莉娟, 陈斌. 中华按蚊血红素过氧化物酶家族基因的全基因组鉴定、特征和进化[J]. , 2018, 61(1): 36-47.

四种重要医学媒介蚊虫离子受体基因IR8a和IR25a的特征及分类地位

Characteristics and classification position of the ionotropic receptor genes IR8a and IR25a in four vector mosquito species of medical importance

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10